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Breeding method of winter wheat by molecular marker-assisted selection generation-adding technology

A molecular marker-assisted, winter wheat technology, applied in botany equipment and methods, plant gene improvement, application, etc., can solve problems that are difficult to put into practical production and application

Pending Publication Date: 2022-07-01
CROP RES INST SHANDONG ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But it is difficult to put into practical production application

Method used

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  • Breeding method of winter wheat by molecular marker-assisted selection generation-adding technology
  • Breeding method of winter wheat by molecular marker-assisted selection generation-adding technology
  • Breeding method of winter wheat by molecular marker-assisted selection generation-adding technology

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Embodiment

[0040] 1. On April 25th, a high-quality combination was prepared at the Jinan base: Daimai 4493 / Jimai 44, making 5 ears, and each ear had about 30 dominant florets.

[0041] 2. The wheat in the hybrid combination is harvested at the wax maturity stage, and sun-dried (the grain moisture content is less than 13%), which is recorded as the F1 generation.

[0042] 3. Select about 30 full germination hybrid seeds, soak them in 1.5% hydrogen peroxide (hydrogen peroxide) for 24 hours, rinse with clean water for 5 times, then wrap the hybrid seeds with moist germination paper and put them at a constant temperature of 2 °C Perform artificial low temperature vernalization in a vernalization room at ~3°C or in a refrigerator freezer.

[0043] 4. Take out after 40 days of artificial low-temperature vernalization, and transplant the F1 generation plants that have undergone artificial low-temperature vernalization into plastic pots (plastic pot size: 40*28*25cm).

[0044] 5. Move the plast...

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Abstract

The invention belongs to the field of crop breeding, and particularly relates to a breeding method of winter wheat by a molecular marker-assisted selection generation-adding technology. The invention provides an efficient winter wheat breeding technology combining a modern biotechnology and a north-south generation adding technology, the breeding accuracy is improved by screening target genes, determining breeding targets and adopting molecular marker-assisted selection, and the breeding process is accelerated by north-south generation adding.

Description

technical field [0001] The invention belongs to the field of crop breeding, in particular to a breeding method of winter wheat molecular marker-assisted selection plus generation technology. Background technique [0002] Wheat is one of the main food crops, and the production of wheat is the key to ensuring food security. The growth period of winter wheat planted in some areas is about 8 months, and it must go through low temperature vernalization before jointing and heading, flowering and fruiting. According to the conventional method, winter wheat can only be reproduced for one generation a year. If you want to cultivate a wheat variety, combined with the breeding cycle of winter wheat, it takes more than 10 years. There have been some technical explorations on how to shorten the breeding period, such as addition generation technology, wheat haploid technology and so on. But it is difficult to put into practical production application. SUMMARY OF THE INVENTION [0003...

Claims

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Application Information

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IPC IPC(8): A01H1/04A01H1/02A01G22/20
CPCA01H1/045A01H1/04A01H1/02A01G22/20
Inventor 李豪圣程敦公郭军曹新有李法计钟文毛瑞喜韩冉翟胜男訾妍刘成刘爱峰宋健民刘建军
Owner CROP RES INST SHANDONG ACAD OF AGRI SCI
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