Molecular markers and screening methods of ppaiv2 gene locus in pear

A molecular marker and gene locus technology, applied in the field of molecular genetics, can solve the problems of long cycle and difficulty, and achieve the effect of high cost, cost saving and clear variation.

Active Publication Date: 2020-11-27
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fruit sugar content belongs to fruit traits, and the traditional hybrid breeding method has a long cycle and great difficulty

Method used

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  • Molecular markers and screening methods of ppaiv2 gene locus in pear

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The screening method of the molecular marker of pear PpAIV2 gene locus comprises the following methods,

[0028] A. Crossbreeding pear PpAIV2 allelic variants as parents to obtain hybrid F1;

[0029] B. Using the CTAB method to extract the DNA of each individual plant of the F1 population, referring to the resequencing results of the parents, using the SSR markers of simple sequence repeats next to PpAIV2 to screen the polymorphic primers of the two parents, and amplify them by polymerase chain reaction. The amplification products were separated and analyzed by electrophoresis on a polyacrylamide gel with a density of 0.06g / ml. According to the molecular marker screening results, the polymorphic primers between the parents were screened out, and the polymorphic primers between the parents were tested in the F1 population. Analysis, polymerase chain reaction amplification procedure is the same as above to obtain population genotype data;

[0030] C. Analyze the genetic ...

Embodiment 2

[0035] The screening method of the molecular marker of pear PpAIV2 gene locus comprises the following methods,

[0036] A. Crossbreeding pear PpAIV2 allelic variants as parents to obtain hybrid F1;

[0037] B. Using the CTAB method to extract the DNA of each individual plant of the F1 population, referring to the resequencing results of the parents, using the SSR markers of simple sequence repeats next to PpAIV2 to screen the polymorphic primers of the two parents, and amplify them by polymerase chain reaction. The amplified products were separated and analyzed by electrophoresis on a polyacrylamide gel with a density of 0.08g / ml. According to the molecular marker screening results, the polymorphic primers between the parents were screened out, and the polymorphic primers between the parents were tested in the F1 population. Analysis, polymerase chain reaction amplification procedure is the same as above to obtain population genotype data;

[0038]C. Analyze the genetic segre...

Embodiment 3

[0041] The screening method of the molecular marker of pear PpAIV2 gene locus comprises the following methods,

[0042] A. Crossbreeding pear PpAIV2 allelic variants as parents to obtain hybrid F1;

[0043] B. Using the CTAB method to extract the DNA of each individual plant of the F1 population, referring to the resequencing results of the parents, using the SSR markers of simple sequence repeats next to PpAIV2 to screen the polymorphic primers of the two parents, and amplify them by polymerase chain reaction. The amplified products were separated and analyzed by electrophoresis on a polyacrylamide gel with a density of 0.07g / ml. According to the molecular marker screening results, the polymorphic primers between the parents were screened out, and the polymorphic primers between the parents were tested in the F1 population. Analysis, polymerase chain reaction amplification procedure is the same as above to obtain population genotype data;

[0044] C. Analyze the genetic segr...

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Abstract

The invention relates to the field of molecular genetics, discloses a molecular marker of a pear PpAIV2 gene locus and further discloses a screening method of the molecular marker of the pear PpAIV2 gene locus. A genome simple sequence repeat (SSR) locus adjacent to pear soluble acid invertase gene PpAIV2 is predicated and a locus specific primer is designed; one SSR allelic variation locus which is tightly interlocked with the PpAIV2 is obtained through amplification in germplasms with different genetic backgrounds and F1-generation population genomes; the SSR allelic variation locus is named as P.zaas-1. The pear soluble acid invertase gene PpAIV2 is a key gene for accumulating pear fruit sucrose; the P.zaas-1 has potential identification and screening effects on materials with remarkable differences on accumulation of the fruit sucrose.

Description

technical field [0001] The invention relates to the field of molecular genetics, relates to a molecular marker of a pear PpAIV2 gene locus, in particular to a screening method for the molecular marker of a pear PpAIV2 gene locus. Background technique [0002] Pear is an important fruit tree in my country, which is a perennial woody plant with a long childhood. Fruit sugar content is one of the most important quality traits, and high sugar content and moderate sugar content are important indicators of high-quality commercial pears. Fruit sugar content is a fruit trait, and the traditional hybrid breeding method has a long cycle and great difficulty. Generally, it takes 3 to 5 years for the results to carry out preliminary identification. Assisted breeding can effectively solve this problem by locating important gene loci for fruit sugar content traits. [0003] Genetic studies have shown that sucrose is one of the main sugars in pear fruit, and the sucrose content is contr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 王月志戴美松蔡丹英施泽彬
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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