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Molecular marker method for simultaneously predicting abdominal fat characters, leg skeleton characters and reproductive performance of broiler chickens and application

A technology of molecular markers and reproductive performance, applied in the field of animal molecular genetics, can solve problems such as prediction and selection of multiple traits that have not yet been discovered, and achieve the effects of balanced breeding of many traits, good reproductive performance, and good economic benefits

Active Publication Date: 2020-08-21
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] So far, the use of molecular markers for prediction and selection is almost always aimed at one trait, and there is no report on the use of one marker to predict and select multiple traits at the same time

Method used

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  • Molecular marker method for simultaneously predicting abdominal fat characters, leg skeleton characters and reproductive performance of broiler chickens and application
  • Molecular marker method for simultaneously predicting abdominal fat characters, leg skeleton characters and reproductive performance of broiler chickens and application
  • Molecular marker method for simultaneously predicting abdominal fat characters, leg skeleton characters and reproductive performance of broiler chickens and application

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Embodiment 1

[0038] Example 1. A molecular marker method for simultaneously predicting abdominal fat traits, leg bone traits and reproductive performance of broilers.

[0039] 1. Design and synthesis of primers.

[0040] Primers were designed according to the SNP site g.3656C>T upstream 208bp and downstream 72bp sequences of the third exon of the chicken SREBP1 gene, and were synthesized by Jinweizhi Biotechnology Co., Ltd. The primer sequences are as follows:

[0041] SREBP1-F:5'-CGGCGTAATGCTGACC-3'

[0042] SREBP1-R:5'-GTGCCACACTCTGCCC-3'

[0043] 2. Using the amplification primers obtained in step 1 to perform PCR amplification of chicken genomic DNA to obtain amplification products.

[0044] DNA extraction, product amplification:

[0045] 1) Extraction of chicken DNA, chicken DNA small sample extraction can adopt the following two methods:

[0046] Method 1: (1) Take 20 μl of anticoagulated blood, add 500 μl of poultry lysate, add proteinase K to a final concentration of 100-200 μg...

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Abstract

The invention discloses a molecular marking method for simultaneously predicting abdominal fat characters, leg skeleton characters and reproductive performance of broilers and an application, and belongs to the technical field of animal molecular genetics. The invention discloses a method for identifying low-fat broilers more accurately and conveniently and accelerating broiler breeding process. According to the invention, primers are designed according to the sequences from the 208 bp at the upstream to the 72 bp at the downstream of the SNP locus g.3656C > T of the chicken SREBP1 gene exon 3to obtain an amplification primer, then carrying out PCR amplification on the chicken genomic DNA according to the obtained primer, and carrying out enzyme digestion on the amplification product by using restriction endonuclease to obtain the chicken marker genotype. The method is simple to operate, low in cost and high in accuracy, can be used for automatic detection, is an effective molecular marker breeding means, and can be used for accelerating the breeding process of chickens and cultivating low-fat broilers with healthy leg bones and good reproductive performance, which meet market requirements, so that good economic benefits are obtained.

Description

technical field [0001] The invention belongs to the technical field of animal molecular genetics, and in particular relates to a molecular marker method and application for simultaneously predicting abdominal fat traits, leg bone traits and reproductive performance of broilers. Background technique [0002] After long-term selective breeding, the growth rate and meat yield of broilers have been significantly improved, but broiler breeding is facing new challenges. Accompanied by rapid growth, physiological discomforts and related diseases of broilers have increased significantly, such as excessive body fat accumulation, decreased reproductive performance, and occurrence of leg diseases. These problems have caused huge economic losses to broiler producers. [0003] Excessive fat deposition (especially abdominal fat) in broilers has many disadvantages: (1) significantly reduces feed conversion efficiency, because fat deposition per unit weight consumes three times more energy ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 王守志李辉王宁张影李玉茂曹志平栾鹏
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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