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Primer group and kit for simultaneously amplifying 37 Y-STR gene loci of human and application thereof

A technology of Y-STR and gene loci, applied in the field of molecular genetics, can solve problems such as insufficiency

Pending Publication Date: 2021-05-28
百特元生物科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, STR typing is applicable to DNA purified by various DNA purification methods, and the amount of DNA obtained by these purification methods is often insufficient for Southern blot analysis

Method used

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  • Primer group and kit for simultaneously amplifying 37 Y-STR gene loci of human and application thereof
  • Primer group and kit for simultaneously amplifying 37 Y-STR gene loci of human and application thereof
  • Primer group and kit for simultaneously amplifying 37 Y-STR gene loci of human and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0121] Direct multiplex amplification of two hairs from suspected father and son detected 37 loci. The amplification method adopts the method of direct amplification: take a section of hair with hair follicles and put it directly into the amplification system so that the hair follicles are completely immersed in the liquid. The amplification procedure adopts the standard amplification procedure of the present invention, and the amplification instrument adopts ABI Proflex PCR instrument, genetic analyzer adopts 3500xl genetic analyzer, analysis software adopts GeneMapper-ID-X analysis software. The operation steps of this embodiment are as follows:

[0122] ①Use scissors to cut the hair short at 0.5cm above the hair follicle, and put the short hair with the hair follicle into a 200μl PCR tube.

[0123] ②A 10 μl amplification system was prepared according to the standard reaction system of the present invention: 2 μl of primer mixture, 4 μl of reaction premix, and 4 μl of deion...

Embodiment 2

[0131] Embodiment 2: the application of kit of the present invention in forensic medicine

[0132] The invention is mainly used in the field of forensic medicine for the construction of the DNA database of criminals and identification of the identity of criminal suspects.

[0133] The national forensic DNA database is a national DNA database of criminals established by the Ministry of Public Security of my country. Now the database has exceeded 50 million data, and it is the main tool for the public security system to detect cases. The database is usually built by the method of direct amplification and detection of blood samples. The identification of illegal and criminal suspects is usually complicated, and the method of amplification and detection after DNA extraction is used for detection. The invention can take into account two different inspection materials, and facilitates the construction of a national DNA database and identification of the identity of criminal suspects...

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Abstract

The invention provides a primer group and a kit for simultaneously amplifying 37 Y-STR gene loci of human and application thereof, and belongs to the technical field of molecular genetics. In the invention, the 37 Y-STR gene loci comprise 36 Y chromosome STR gene loci and 1 Y-indel gene locus; 37 gene loci can be amplified in one reaction at the same time, the compatibility of current public security DNA database comparison is fully met, the amplification time is shortened, the identification efficiency and the detection material adaptability of the kit are improved, and the multifunctional STR identity identification requirements of current forensic identity identification, forensic DNA database construction and judicial genetic relationship identification can be met.

Description

technical field [0001] The invention relates to the technical field of molecular genetics, in particular to a primer set, a kit and an application thereof for simultaneously amplifying 37 human Y-STR loci. Background technique [0002] STR (short tandem repeats, short tandem repeats), also known as microsatellite sequence, is a short tandem repeat sequence widely present in human genome DNA, and the repeat unit is 2 to 6 nucleotides. Due to its high polymorphism and stability, and compared with AMP-FLP and VNTR genotyping methods, the amplified product length of STR genotyping method is much smaller (less than 500bp), so the requirement for template quality is lower , even degraded DNA templates can be analyzed. In addition, STR typing is applicable to DNA purified by various DNA purification methods, but the amount of DNA obtained by these purification methods is often not enough for Southern blot analysis. In view of the above characteristics, STR typing technology has b...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156
Inventor 冉凌飞蒿杰刘甲乾
Owner 百特元生物科技(北京)有限公司
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