SNP (Single Nucleotide Polymorphism) marker for influencing wool length of alpine merino sheep and application of SNP marker
A Merino sheep and Alpine technology, applied in the field of molecular genetics, can solve the problems that important genetic resources have not been effectively excavated and utilized, and the molecular marker system for fine-haired wool traits has not been established, so as to achieve easy result interpretation and improve selection. The effect of high accuracy and high accuracy
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[0036] Example 1 Correlation between different genotypes and wool length of Alpine Merino sheep
[0037] 1. Sample Collection
[0038] The samples were from the sheep breeding technology extension station in Gansu Province. Blood samples were collected from 116 Alpine Merino sheep with production records. 5 mL of venous blood was collected from each sheep in a blood collection tube with EDTA-K2 anticoagulant. After the blood samples were collected, they were quickly mixed. , put it in a sampling box containing an ice pack for temporary storage, and then transport it back to the laboratory and store it in a -20°C refrigerator for DNA extraction. The wool length record of each sheep was provided by the Gansu Provincial Sheep Breeding Technology Extension Station.
[0039] 2. Main reagents and instruments
[0040] EDTA-K2 vacuum blood collection tube was purchased from Jiangsu Yuli Medical Instrument Co., Ltd.; blood genome extraction kit was purchased from Tiangen Biochemical ...
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