SNP (Single Nucleotide Polymorphism) marker for influencing wool length of alpine merino sheep and application of SNP marker

A Merino sheep and Alpine technology, applied in the field of molecular genetics, can solve the problems that important genetic resources have not been effectively excavated and utilized, and the molecular marker system for fine-haired wool traits has not been established, so as to achieve easy result interpretation and improve selection. The effect of high accuracy and high accuracy

Active Publication Date: 2022-07-15
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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Problems solved by technology

In recent years, fine-wool sheep genetics and breeding experts at home and abroad have been devoting themselves to the research of candidate genes or molecular markers controlling wool traits, but no molec...

Method used

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  • SNP (Single Nucleotide Polymorphism) marker for influencing wool length of alpine merino sheep and application of SNP marker
  • SNP (Single Nucleotide Polymorphism) marker for influencing wool length of alpine merino sheep and application of SNP marker
  • SNP (Single Nucleotide Polymorphism) marker for influencing wool length of alpine merino sheep and application of SNP marker

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Embodiment 1

[0036] Example 1 Correlation between different genotypes and wool length of Alpine Merino sheep

[0037] 1. Sample Collection

[0038] The samples were from the sheep breeding technology extension station in Gansu Province. Blood samples were collected from 116 Alpine Merino sheep with production records. 5 mL of venous blood was collected from each sheep in a blood collection tube with EDTA-K2 anticoagulant. After the blood samples were collected, they were quickly mixed. , put it in a sampling box containing an ice pack for temporary storage, and then transport it back to the laboratory and store it in a -20°C refrigerator for DNA extraction. The wool length record of each sheep was provided by the Gansu Provincial Sheep Breeding Technology Extension Station.

[0039] 2. Main reagents and instruments

[0040] EDTA-K2 vacuum blood collection tube was purchased from Jiangsu Yuli Medical Instrument Co., Ltd.; blood genome extraction kit was purchased from Tiangen Biochemical ...

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Abstract

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP (Single Nucleotide Polymorphism) marker related to influence on the wool length of alpine merino and application of the SNP marker, and the SNP molecular marker is located at the 6296384 nucleotide site T/C mutation on the No.3 chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the wool length of the high-mountain merino sheep, shortening the breeding period and accelerating the breeding process, a technology for selecting the wool length of the high-mountain merino sheep in the early stage is established, the breeding time of excellent characters of the wool length of the high-mountain merino sheep is shortened, the breeding cost is reduced, and the application value is very high.

Description

technical field [0001] The invention belongs to the technical field of molecular genetics, and in particular relates to SNP markers affecting the wool growth of Alpine Merino sheep and applications thereof. Background technique [0002] Wool refers to a layer of textile-worthy fibers covering the surface of the sheep's body and is a derivative of the skin. Wool is the main raw material of wool spinning industry, accounting for about 97% of wool spinning raw materials. In wool production and breeding practice, wool traits are important economic traits, mainly including hair length, wool yield, fiber diameter, bending, breaking strength, elongation, net wool rate, shearing amount and other indicators, which are closely related to weaving. Products and economic benefits are closely related. Due to population growth, demand for wool has exceeded supply. As market demands change, producing high-quality wool has become the main goal of goat breeding. [0003] Alpine Merino she...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/156C12Q2600/124C12Q2531/113C12Q2565/125Y02P60/87
Inventor 袁超卢曾奎郭婷婷杨博辉李建烨刘建斌岳耀敬牛春娥孙晓萍
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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