Molecular marking method for peanut flowering habit related gene locus and application of molecular marking method

A technology of flowering habits and molecular markers, applied in the fields of crop molecular genetics and molecular breeding

Active Publication Date: 2020-07-31
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing studies have not found a unified understanding, and there is no more accurate positioning of the genetic loci related to peanut flowering habit and the development of molecular marker-assisted selection markers

Method used

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  • Molecular marking method for peanut flowering habit related gene locus and application of molecular marking method
  • Molecular marking method for peanut flowering habit related gene locus and application of molecular marking method
  • Molecular marking method for peanut flowering habit related gene locus and application of molecular marking method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Genetic Mapping of Genetic Loci Related to Flowering Habits in Hybrid Populations of Monogenic Inheritance Models

[0047] (1) Materials and methods:

[0048] (1) Construction of plant materials and genetic populations: The continuous flowering cultivated peanut Pingdu 9616 and the alternate flowering cultivated peanut Florunner preserved in the Peanut Center Laboratory of Qingdao Agricultural University were used as test materials, and the continuous flowering cultivated peanut Pingdu 9616 and alternately flowering cultivated peanut were used as test materials. Flowering cultivated peanut Florunner hybrid, planting hybrid F 1 , selfing to obtain F 2 Separation of populations, continuous self-breeding of multiple generations of strains, and finally the formation of F 7 Recombinant inbred line RIL-PF-F 7 .

[0049] (2) The population genomic DNA was extracted by the improved CTAB method (for details, refer to the "Molecular Cloning Experiment Guide").

[...

Embodiment 2

[0063] Example 2: Development and application of locus-assisted selection markers related to flowering habit in monogenic genetic model populations

[0064] With flatness 9616, Florunner and RIL-PF-F thereof in embodiment 1 7 The genomic DNA of the group individual is used as a template, and a pair of primers or two pairs of primers in the pair of primers labeled PID12-21 and PID12-29 are used to perform PCR amplification on the above template on a PCR machine, and 1-1.5 μL of amplified The amplified product was detected by electrophoresis on an 8% non-denaturing polyacrylamide gel with a voltage of 300V and a current of 280mA for 1-1.5 hours. The length of electrophoresis can be appropriately adjusted according to the size of the target fragment. The gel was fixed, infiltrated, rinsed and developed to develop the gel silver stain.

[0065] The physical position of the marker PID12-21 is located at the end of chromosome 12 of the peanut reference genome Tifrunner, and its cor...

Embodiment 3

[0076] Example 3: Genetic Mapping of Genetic Loci Related to Flowering Habits in Hybrid Populations of Digene Inheritance Model

[0077] (1) Materials and methods:

[0078] (1) The alternate flowering cultivated peanut Xiaohongmao and the continuous flowering cultivated peanut Henan Nanyang preserved in the Peanut Center Laboratory of Qingdao Agricultural University were used as parents, and hybridization was carried out to plant the hybrid F 1 , selfing to obtain F 2 Separation of populations, continuous self-breeding of multiple generations of strains, and finally the formation of F 8 Recombinant inbred line RIL-HN-F 8 .

[0079] (2) The population genomic DNA was extracted by the improved CTAB method (for details, refer to the "Molecular Cloning Experiment Guide").

[0080] (3) Development of polymorphic InDel markers: Based on the resequencing data of Xiaohongmao and Henan Nanyang peanut genomes and the reference genomes of Tifrunner and Shitouqi downloaded from the NC...

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Abstract

The invention belongs to the technical fields of molecular genetics and molecular breeding of crops and provides a molecular marking method for a peanut flowering habit related locus and application of the molecular marking method. A molecular marker comprises InDel markers PID12-21 and PID12-29 which are located on a twelfth chromosome and an InDel marker PID2-9 located on a 02-th chromosome. Formonogenic-inheritance hybrid colonies, flowering habits only need to consider a genotype of flowering habit related loci of the twelfth chromosome; for digenic-inheritance hybrid colonies, the flowering habits need to comprehensively consider genotypes of the flowering habit related loci of the 02-th and the twelfth chromosomes; and regardless of the hybrid colonies of monogenic-/digenic-inheritance modes, by only identifying a genotype of an amplified target strap, a perpetual blooming type and an alternate blooming type of peanuts can be distinguished, types of flowering habits of filial generations of the peanuts are screened rapidly, and flowering habit related plant type breeding, yield breeding and the like are assisted.

Description

Technical field: [0001] The invention belongs to the technical field of crop molecular genetics and molecular breeding, and relates to a molecular marker-assisted selection method for peanut flowering habit-related gene loci, which is used for molecular marker-assisted improvement of peanut flowering habit-related traits, and further realizes the number of peanut branches, Molecular marker-assisted improvement of plant type and yield. Background technique: [0002] Peanut (Arachis hypogaea L.) is one of the important oil crops in my country. It is rich in various nutrients needed by the human body, such as unsaturated fatty acids, protein and trace elements. Cultivated peanut (allotetraploid AABB, 2n=4x=40) is domesticated after natural outcross doubling of two diploid wild species with different genome types. Its genome structure is relatively complex, and genetic research has been carried out relatively late. . With the publication of the whole genome sequencing results ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156Y02A40/146
Inventor 张晓军李季华郭蕊于晓娜司彤邹晓霞王月福王铭伦
Owner QINGDAO AGRI UNIV
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