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A molecular marker method and application of peanut flowering habit-related gene loci

A technology of flowering habit and gene locus, which is applied in the field of molecular genetics and molecular breeding of crops to save costs, improve the efficiency of breeding, and speed up the breeding speed.

Active Publication Date: 2022-05-31
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Existing studies have not found a unified understanding, and there is no more accurate positioning of the genetic loci related to peanut flowering habit and the development of molecular marker-assisted selection markers

Method used

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  • A molecular marker method and application of peanut flowering habit-related gene loci
  • A molecular marker method and application of peanut flowering habit-related gene loci
  • A molecular marker method and application of peanut flowering habit-related gene loci

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] (2) using the improved CTAB method to extract the population genomic DNA (specifically refer to "Molecular Cloning Experiment Guide").

[0052]

Embodiment 2

[0071] The PCR reaction system is 10 μl, of which 12.5 μl of 2×Taq Master Mix for PAGE, 1 μl of template, 10 μM

Embodiment 3

[0079] (2) Extract the population genomic DNA with the improved CTAB method (specifically refer to the "Molecular Cloning Experiment Guide").

[0084]

[0085]

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PUM

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Abstract

The invention belongs to the technical field of crop molecular genetics and molecular breeding, and provides a molecular marker method and application of peanut flowering habit-related sites. The molecular markers include InDel markers PID12‑21 and PID12‑29 located on the 12th The InDel marker PID2‑9 of chromosome 02; the flowering habit of the single-gene inheritance hybrid population only needs to consider the genotype of the locus related to the flowering habit of chromosome 12; The genotype of the locus related to the flowering habit of the chromosome; whether it is a hybrid population with a single or double gene inheritance model, only the genotype of the amplified target band can be identified to distinguish the continuous flowering type and the alternate flowering type of peanuts, Quickly screen the types of flowering habits of peanut hybrid offspring, and assist in plant type breeding and yield breeding related to flowering habits.

Description

A molecular marker method for peanut flowering habit related gene loci and its application Technical field: The invention belongs to crop molecular genetics and molecular breeding technical field, relate to peanut flowering habit related gene locus Point-based molecular marker-assisted selection method for molecular marker-assisted improvement of peanut flowering habit-related traits, further To achieve molecular marker-assisted improvement of peanut branch number, plant type and yield. Background technique: Peanut (Arachis hypogaea L.) is one of the important oil crops in my country, and it is rich in many Nutrients such as unsaturated fatty acids, protein and trace elements. Cultivated peanut (allotetraploid AABB, 2n=4x= 40) was domesticated by natural outcross doubling of two diploid wild species with different genome types, and their genome structures are similar. For complex, genetic studies were carried out relatively late. With the current whole genome seq...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156Y02A40/146
Inventor 张晓军李季华郭蕊于晓娜司彤邹晓霞王月福王铭伦
Owner QINGDAO AGRI UNIV
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