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Method for identifying chicken abdominal fat mass by using chicken CEBP zeta gene and application of method

A technology for identifying chicken belly fat, which is applied in the field of animal molecular genetics, can solve problems that have not yet been discovered, achieve high accuracy, low cost, and accelerate the breeding process

Active Publication Date: 2021-02-19
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no report on the selection of chicken belly fat content by CEBPζ gene molecular markers

Method used

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  • Method for identifying chicken abdominal fat mass by using chicken CEBP zeta gene and application of method
  • Method for identifying chicken abdominal fat mass by using chicken CEBP zeta gene and application of method
  • Method for identifying chicken abdominal fat mass by using chicken CEBP zeta gene and application of method

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Experimental program
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Embodiment 1

[0028] Example 1. Molecular marker method for identifying chicken belly fat mass

[0029] 1. Design and synthesis of primers:

[0030] Primers were designed according to the SNP site g.764T>G upstream 123bp downstream 302bp sequence of the chicken CEBPζ gene, and were synthesized by Handsome Biotechnology Co., Ltd. The primer sequence is as follows:

[0031] The sequence of CEBPZ-F is shown in SEQ ID NO.1, that is, 5'-CGTGCTAGGGAGGTGATA-3';

[0032] The sequence of CEBPZ-R is shown in SEQ ID NO.2, that is, 5'-TGGACGCTGTGAGAAAGA-3'.

[0033] 2. Utilize the amplification primers gained in step 1 to carry out the PCR amplification of chicken genomic DNA to obtain the amplified product:

[0034] DNA extraction, product amplification:

[0035] 1) Extraction of chicken DNA, chicken DNA small sample extraction can adopt the following two methods:

[0036] Method 1: (1) Take 20 μl of anticoagulated blood, add 500 μl of poultry lysate, add proteinase K to a final concentration of 1...

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Abstract

The invention discloses a method for identifying chicken abdominal fat mass by using a chicken CEBP zeta gene and application of the method, belongs to the technical field of animal molecular genetics, and aims to more accurately and conveniently identify low-fat broiler chickens and accelerate the breeding process of the broiler chickens. Primers are designed according to upstream 123bp and downstream 302bp sequences of the chicken CEBP zeta gene SNP (single nucleotide polymorphism) locus g.764T>G, the obtained primers are used for performing PCR (polymerase chain reaction) amplification on chicken genome DNA, an amplification product is subjected to enzyme digestion by restriction endonuclease, an enzyme digestion product is subjected to agarose gel electrophoresis separation, and a marker genotype of chicken abdominal fat content is obtained. The method is simple to operate, low in cost and high in accuracy, can be used for automatic detection, is an effective molecular marker breeding means, and provides a scientific basis for marker-assisted selection of chickens, and with adoption of the method, acceleration of the broiler chicken breeding process and cultivation of low-fat broiler chickens meeting market requirements are facilitated, so that relatively good economic benefits are obtained.

Description

technical field [0001] The invention belongs to the technical field of animal molecular genetics, and in particular relates to a method for identifying chicken abdominal fat mass by using the chicken CEBPζ gene and an application thereof. Background technique [0002] After long-term selective breeding, the growth rate and meat yield of broilers have been significantly improved, but broiler breeding is facing new challenges. Accompanied by rapid growth, physiological discomforts and related diseases of broiler chickens have increased significantly, such as excessive body fat accumulation, ascites syndrome, sudden death syndrome, leg diseases, and decreased immune function of the body. These problems have caused huge harm to broiler chicken producers. Economic losses. Excessive accumulation of body fat (especially abdominal fat) in broilers has become a prominent problem. Excessive fat deposition in broilers has many disadvantages: (1) significantly reduces feed conversion ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6858C12Q1/6888
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172C12Q2521/313C12Q2531/113C12Q2565/125
Inventor 王守志李紫薇李玉冬刘鑫王伟佳李辉王宁郭怀顺肖凡高海河
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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