Group-of-soybean quantitative trait QTL loci and screening method thereof

A technology of quantitative traits and screening methods, applied in the field of molecular genetics, can solve problems such as phenotypic differences and gene loci that cannot be directly analyzed

Pending Publication Date: 2020-06-26
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The BSA method requires significant phenotypic differences in the segregated population, and is more inclined to perform preliminary positioning of single gene...

Method used

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  • Group-of-soybean quantitative trait QTL loci and screening method thereof
  • Group-of-soybean quantitative trait QTL loci and screening method thereof
  • Group-of-soybean quantitative trait QTL loci and screening method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 QTL mapping analysis of soybean protein content: BC1 population constructed by Heihe 50 and Zhongyin 1106

[0038] 1) Screening of parents

[0039] Heihe 50 from the Heihe Branch of Heilongjiang Academy of Agricultural Sciences and Zhongyin 1106, which were bred from Heilongjiang Academy of Agricultural Sciences, and Zhongyin 1106 were selected. Heihe 50 was used as the female parent. The male parent is mainly characterized by late maturity and lodging resistance, with a protein content of 45.88%.

[0040] 2) Genetic population construction

[0041] In 2011, both Heihe 50 and Zhongyin 1106 were planted in the experimental base of Heihe City, Heilongjiang Province, artificially pollinated and crossed, and obtained F 1 , planted F in the experimental field of Heihe Branch in 2012 1 , planted F in 2013 2 , from which a single plant with a protein content of 45.79% was selected and crossed with Heihe 50 to construct a backcross population, and the BC 1 f 1 ,...

Embodiment 2

[0077] Example 2 QTL mapping analysis of soybean protein content: RIL population constructed by Zhonghuang 35 and Tokachi Changye

[0078] 1) Screening of parents

[0079] The high-oil and high-yield Zhonghuang 35 was selected as the female parent, and the protein content was 40.31% and 39.25% respectively for two consecutive years; the high-protein variety Tokachi Changye introduced from Japan was used as the male parent, and the protein content was 46.21% for two consecutive years. % and 44.47%.

[0080] 2) Genetic population construction

[0081] F 2 group. Using the method of single-grain transmission, we carried out additional generation breeding in Beijing and Hainan, and formed a stable recombinant inbred line population consisting of 199 families (F 15 and F 16 ).

[0082] 3) Determination and distribution of protein content

[0083] The protein content assay method is the same as in Example 1.

[0084] Using SPSS19.0 to F 15 and F 16 The protein content of t...

Embodiment 3

[0104] Example 3 QTL mapping analysis of soybean branch number

[0105] 1) Screening of parents

[0106] The multi-branched variety KN24 was selected as the female parent and the less-branched variety KF19 was used as the male parent to prepare a hybrid combination. In 2015, the number of branches of KN24 was 6.0, and that of KF19 was 0.6; in 2016, the number of branches of KN24 was 7.9 , and the number of branches of KF19 is 1.1.

[0107] 2) Genetic population construction

[0108] F 1 , selfing produces F 2 , F 2 by 3 strains of F 1 produced by selfing, F 2 A total of 549 individual plants were planted in Keshan County, Heilongjiang Province, under conventional field management. After the soybeans matured, the side plants were removed, and the agronomic traits of each individual plant, such as plant height, number of main stem nodes, and number of branches, were measured.

[0109] 3) Identification and distribution of branch numbers

[0110] KN24×KF19 population F 2...

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Abstract

The invention discloses group-of-soybean quantitative trait QTL loci and a screening method thereof, and belongs to the field of molecular genetics. The method comprises the following steps: determining a target character; screening parents and constructing a genetic population; obtaining phenotypic data of the target character; extracting genome DNA of the parent and the genetic population; constructing a mixing pool, further screening molecular markers linked with target traits between parents and the mixing pool, and selecting the molecular markers with p less than 0.0001 to further identify genotypes in the genetic population; performing qTL localization analysis. By utilizing the method, two QTL loci for controlling the content of soybean protein and QTL loci for controlling the branching number of soybeans are screened out. The soybean quantitative trait QTL locus screening method is established, a foundation is laid for gene initial positioning, fine positioning, cloning and newvariety breeding of soybean related traits, and meanwhile available markers are provided for soybean molecular marker-assisted selection.

Description

technical field [0001] The invention relates to the technical field of molecular genetics, in particular to a group of soybean quantitative trait QTL loci and a screening method thereof. Background technique [0002] The traits of animals and plants are generally divided into qualitative traits and quantitative traits. Quantitative traits are controlled by major genes, and the effect is relatively large, while quantitative traits are controlled by multiple genes, and the effect of each gene is small. Bulked Segregant Analysis (BSA) is a conventional method for locating single genes of qualitative traits or major genes of quantitative traits, but this method is powerless for locating minor genes. [0003] The BSA method is evolved from the near-isogenic lineage analysis method, and it can also quickly and efficiently perform gene mapping when there is no genetic linkage map or the map saturation is low. The BSA method requires significant phenotypic differences in segregate...

Claims

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Application Information

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IPC IPC(8): G16B20/30
CPCG16B20/30
Inventor 邱丽娟王婉武阳春张勇索比苏伯鸿
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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