A kind of rflp method and its application of quick detection yellow cattle flii gene SNP

A scalper and gene technology, applied in the field of molecular genetics, can solve problems such as no related reports, and achieve the effect of low cost and accurate detection

Active Publication Date: 2020-01-21
NORTHWEST A & F UNIV
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Problems solved by technology

[0006] At present, there are no relevant reports at home and abroad on the research on the genetic variation of FLII gene in cattle

Method used

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  • A kind of rflp method and its application of quick detection yellow cattle flii gene SNP
  • A kind of rflp method and its application of quick detection yellow cattle flii gene SNP
  • A kind of rflp method and its application of quick detection yellow cattle flii gene SNP

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Embodiment Construction

[0023] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0024] The present invention uses the PCR-RFLP method to detect the single nucleotide polymorphism at position 9461 of the cattle FLII gene, and performs correlation analysis with growth traits to verify whether it can be used as a molecular marker for auxiliary selection in cattle molecular breeding, thereby accelerating The speed of breeding of improved varieties. The present invention detects and analyzes the gene frequency of the SNP genotypes of four cattle breeds, and conducts correlation analysis between the above SNP site and the important growth traits of the cattle, and the results show that the site can be used as a molecular marker for improving the growth traits of the cattle.

[0025] 1. Cattle sample collection

[0026] The present invention specifically takes the population of 4 local yellow cattle breeds in China as the detection obj...

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Abstract

The invention discloses an RFLP method for fast detecting FLII genes of cattle and application thereof. The method includes the steps that with cattle genome DNA as a template and a primer pair P as primers, PCR amplification is performed on an intron 22 area of the FLII genes of the cattle, PCR amplification products are digested through restriction enzyme Sac II, then agarose gel electrophoresis is performed on the enzyme-digested segments, the single-nucleotide polymorphism of the intron 22 area of the FLII genes of the cattle is identified according to an agarose gel electrophoresis result, an SNP locus is remarkably relevant with the growth and development character of the cattle and can serve as a molecular genetics marker closely relevant to the growth character of the cattle in aspects of DNA level screening and detection, the RFLP method is applied to auxiliary selection of marking of the meat type growth character of the cattle, and therefore a cattle population good in genetic resource can be fast established.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and in particular relates to a method for detecting the single nucleotide polymorphism at position 9461 of the cattle FLII gene. Background technique [0002] Single nucleotide polymorphism (single nucleotide polymorphism, SNP) refers to the polymorphism caused by the change of a single nucleotide (A / T / C / G) in the genomic DNA sequence. Its variant forms include: transversion, conversion, insertion and deletion, etc., which are mainly caused by the conversion or transversion of a single base. SNP belongs to the third-generation molecular marker, which has the advantages of high density, biallelic genes, and easy automatic detection. Because SNP has the incomparable advantages of other markers, it has been widely used as a research tool in various fields of life science. [0003] At present, several different routes are mainly used to discover SNPs, namely DNA sequence determination method, PCR-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6858C12Q1/6888
CPCC12Q1/6858C12Q1/6888C12Q2600/124C12Q2600/156C12Q2521/313C12Q2531/113
Inventor 陈宏刘梅刘敏蓝贤勇黄永震白跃宇
Owner NORTHWEST A & F UNIV
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