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Single nucleotide polymorphism of GHRHR genes in dairy goat and detection method thereof

A single nucleotide polymorphism and detection method technology, applied in the field of single nucleotide polymorphism and its detection of dairy goat GHRHR gene, to achieve accurate estimation, speed up breeding progress, and improve selection efficiency

Inactive Publication Date: 2012-06-27
NORTHWEST A & F UNIV
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Problems solved by technology

At present, the research on the function of this gene locus and the association between its genetic variation and economic traits (such as: milk production in different lactation periods) is still blank

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  • Single nucleotide polymorphism of GHRHR genes in dairy goat and detection method thereof
  • Single nucleotide polymorphism of GHRHR genes in dairy goat and detection method thereof
  • Single nucleotide polymorphism of GHRHR genes in dairy goat and detection method thereof

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Embodiment Construction

[0031]The present invention designs primers according to the conserved sequence of the GHRHR gene of the homologous animal cow of the dairy goat, uses the genomic DNA pools of two dairy goat varieties as templates, performs PCR amplification, purifies the PCR products, and searches for the sequence after sequencing. The single nucleotide polymorphism of the amplified fragment; the trait association analysis is carried out for the found single nucleotide polymorphism, and its detection method is provided, making the nucleotide polymorphism of the GHRHR gene a fast and convenient detection method. Molecular genetic markers to provide a basis for accelerating the establishment of dairy goat populations with high-quality economic traits.

[0032] a. Cloning of partial DNA sequence of dairy goat GHRHR gene and detection of its polymorphism

[0033] 1. Collection and processing of dairy goat blood samples

[0034] Take 10 mL of goat blood, add 500 μL of 0.5 mol / L EDTA to anticoagul...

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Abstract

The invention discloses single nucleotide polymorphism of GHRHR genes in dairy goat and a detection method thereof. The single nucleotide polymorphism of the genes comprises C / G base polymorphism which is the 231st in the sequence table of the GHRHR gene in the dairy goat shown in SEQ ID NO.1. The detection method comprises the following steps: taking the DNA of the whole genome of the dairy goat to be detected containing the GHRHR genes as a template and a primer pair P as a primer to carry out PCR amplification on the GHRHR genes in the dairy goat; after using restriction enzyme MspI to digest the PCR amplified product, carrying out agarose gel electrophoresis on amplified fragments after restriction enzyme; and identifying the polymorphism of the genes according to the electrophoresis results. The method screens and detects the molecular genetic markers closely related to the milk production traits of the dairy goat on the DNA level to be used for assisted selection and molecular breeding of the dairy goat and speed up find breeding of the dairy goat.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and relates to using the single nucleotide polymorphism (SNP) of the functional gene of the dairy goat as a molecular genetic marker, in particular to the single nucleotide polymorphism of the GHRHR gene of the dairy goat and a detection method thereof. Background technique [0002] Goat milk has high nutritional value, does not contain allergens, has balanced nutritional components, is easy to digest and absorb, and is similar to human milk in protein composition; the volume of fat particles in goat milk is one-third that of milk, and the protein, minerals The content of substances, especially calcium, phosphorus, vitamins and trace elements is slightly higher than that of milk, and it is more conducive to human body absorption. It is known as the "king of milk" in the international nutrition circle. Fewer people drink goat's milk than goat's milk because of its smell and taste. With the appli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 陈宏刘艳丽蓝贤勇屈玉娇李转见陈忠琦雷初朝胡沈荣
Owner NORTHWEST A & F UNIV
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