Single nucleotide polymorphism of ox TAS1R2 gene and detecting method thereof

A single nucleotide polymorphism and detection method technology, applied in the field of single nucleotide polymorphism and its detection of cattle TAS1R2 gene, can solve problems such as lack of research, improve selection efficiency, accurately estimate, and accelerate breeding progress Effect
CN101962683BInactive Publication Date: 2013-01-23XUZHOU NORMAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
XUZHOU NORMAL UNIVERSITY
Publication Date
2013-01-23
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a single nucleotide polymorphism of an ox TAS1R2 gene and a detecting method thereof. The detecting method of the single nucleotide polymorphism of the gene comprises the following steps of: with an ox total genome DNA to be detected, which comprises a TAS1R2 gene, as a template and with a primer pair P as a primer, carrying out PCR (Polymerase Chain Reaction) amplification on the ox TAS1R2 gene; after denaturing the product, carrying out polyacrylamide gel electrophoresis on a denatured amplification fragment; and identifying the 288bp base polymorphism of the ox TAS1R2 gene according to an electrophoresis result. The method is used for screening and detecting molecular genetic markers which are closely relevant to ox meat producing characters at a DNA level to beused for the assisted selection and molecular breeding of oxen and improving the breeding speed of fine breeds of the oxen.
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Description

technical field

[0001] The invention belongs to the field of molecular genetics, and relates to using the single nucleotide polymorphism (SNP) of the functional gene of cattle as a molecular genetic marker, in particular to a single nucleotide polymorphism of the TAS1R2 gene of cattle and a detection method thereof. Background technique

[0002] In recent years, my country's beef cattle production has developed rapidly, but the production level is low, and the average meat production rate is not high. One of the important reasons is that my country has not yet cultivated a specialized beef cattle breed. In order to vigorously develop beef cattle production in our country, we must cultivate our own beef cattle breeds in addition to using foreign excellent beef cattle breeds to improve local cattle in our country.

[0003] As an important part of the treasure house of cattle breed resources in the world, my country's yellow cattle breed resources are very rich, with strong ada...

Claims

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