Gene locus closely associated with level of milk polyunsaturated fatty acid and application thereof

A technique for unsaturated fatty acids and gene loci, applied in the field of detecting said gene loci

Inactive Publication Date: 2013-08-07
JILIN UNIV
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Problems solved by technology

The current research mainly focuses on the Caucasian population. Whether the PUFA levels of Chinese pregnant women and lactating mothers are also affected by FADS gene polymorphisms has not been reported yet.

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  • Gene locus closely associated with level of milk polyunsaturated fatty acid and application thereof
  • Gene locus closely associated with level of milk polyunsaturated fatty acid and application thereof
  • Gene locus closely associated with level of milk polyunsaturated fatty acid and application thereof

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[0030] The following examples are further explanations of the present invention.

[0031] 5ml of anticoagulant blood was drawn from the peripheral vein of the subjects, and the wizard was used to ? Genomic DNA Purification Kit was used to extract genomic DNA from whole blood. The steps are as follows: Add 900 μl of cell lysate to a 1.5ml Eppendorf centrifuge tube, add 300 μl of melted whole blood, blow and mix, react at room temperature for 20 minutes, centrifuge at 13,000 rpm for 3 minutes, discard the supernatant, add 300 μl of nuclear lysate, fully shake and repeatedly pipette Until the clumps are dissolved, add 1.5 μl RNase in a water bath at 37°C for 30 minutes and mix thoroughly, add 100 μl protein precipitation solution in a 37°C water bath for 15 minutes, mix well, react at room temperature for 20 minutes, centrifuge at 13,000 rpm for 3 minutes, transfer the supernatant to 300 μl In a centrifuge tube of isopropanol, invert and mix repeatedly, react at room tempe...

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Abstract

The invention relates to a gene locus closely associated with the level of milk polyunsaturated fatty acid and application thereof, belonging to the technical field of genetics. The invention provides a gene locus closely associated with the level of milk polyunsaturated fatty acid, namely a fatty acid desaturase 1 gene (FADS1) locus, i.e. an FADS1 locus having rs174556 mononucleotide polymorphism. The mononucleotide polymorphism rs174556 base C of the locus is mutated into T, thus being closely associated with the level of milk polyunsaturated fatty acid. The levels of arachidonic acid and eicosapentaenoic acid in milk of a wet nurse carrying FADS1 gene rs174556T / T minor allele homozygote are obviously degraded, and the locus has mononucleotide polymorphism determining the level of milk polyunsaturated fatty acid. The FADS1 locus and a coding protein thereof are of great importance in elucidating a polyunsaturated fatty acid metabolic mechanism, establishing individual nutrition intervention and establishing appropriate dietary recommended intakes of polyunsaturated fatty acid based on different genetic backgrounds.

Description

technical field [0001] The invention belongs to the technical field of genetics, and relates to a gene locus closely related to milk polyunsaturated fatty acid level, a method for detecting the gene locus, and an application of the gene locus. Background technique [0002] The nutrition of pregnant women and breast milk is the key to ensure the health of fetus and baby. Fatty acids in breast milk are not only an important source of energy for infants, but also long-chain polyunsaturated fatty acids (LCPUFA) are closely related to infant visual function and brain development. The n-3 series LCPUFAs are mainly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and the n-6 series LCPUFAs are mainly linoleic acid and arachidonic acid (AA). AA, EPA and DHA are the three most important LCPUFAs, which are essential fatty acids for early human development. At the end of pregnancy and the first few months of the newborn's birth, the number of brain cells increases rapidly,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68
Inventor 谢林刘国良甘振威杨镜玉邓娟何纯川姜珊
Owner JILIN UNIV
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