Primer group, kit and method for DNA file library building

Abstract

The invention discloses a primer group, a kit and a method for DNA file library building. The primer group aims at 25 autosomal STR gene loci, 1 X and Y chromosome homologous gene locus, AMEL, 27 Y-STR gene loci, 25 X-STR gene loci, 33 global population common autosomal SNP loci, 28 East Asia population common Y chromosome SNP loci and 3 mitochondrial hypervariable regions. Through advanced screening, the loci can meet various judicial or forensic identification purposes, the cumulative individual recognition rate reaches 0.99999999999999, and the individual recognition application of ChineseHan nationality and most people in the world can be met. Primers are as shown in SEQ ID NO.:1-284. The primer group can be directly used for multiplex PCR, and the genetic marker information of a detected material can be obtained to the maximum extent only by one round of experiment in combination of the multiplex PCR and next-generation sequencing technology. The primer group is suitable for application scenes of newborn birth medicine proving DNA file library building, paternity test, DNA comparison, population genetics research and the like, is also suitable for severely degraded and decayed sample detection, and has important value.

Description

technical field [0001] The invention relates to a primer set, a kit and a method for building a DNA archive. Background technique [0002] As early as 1989, at the Appraisal Meeting of my country's Fingerprint Technology Achievements, forensic experts, leaders of the Ministry of Public Security and the Ministry of Science and Technology proposed the idea of ​​establishing a "DNA fingerprint database". In 2005, the DNA database of my country's public security system was put into operation. After more than ten years of development, the data capacity of the DNA database has increased dramatically. As of May 2016, the total number of DNA databases of public security organs across the country reached 44 million. In 2015, more than 170,000 cases of various types were solved using DNA detection technology, and 3,555 children who were abducted many years ago were successfully helped to find their biological parents. DNA database has become an important tool for public security orga...

AI Technical Summary

Problems solved by technology

However, these technologies also have too many detection sites, the selection of some detection sites lacks specificity or the individual identification ability is not high, resulting in high detection costs, and at the same time, the amount of data is too large, which is difficult for subsequent data analysis and the establishment of forensic standards. It also brings troubles; after PCR captures the target locus or region, additional library construction is required, the experimental process is cumbersome, and the detection cost is also high

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