SNP (Single Nucleotide Polymorphism) molecular marker for influencing wool length character of alpine merino and application of SNP molecular marker
A molecular marker and Merino sheep technology, applied in the field of molecular genetics, can solve the problems that the molecular marker system of fine-haired wool traits has not been established, and important genetic resources have not been effectively excavated and utilized, so as to achieve easy interpretation of results and improve The effect of breeding accuracy and low cost
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[0035] Example 1 Correlation Between Different Genotypes and Hair Length of Alpine Merino Sheep
[0036] 1. Sample Collection
[0037] The samples came from the Sheep Reproduction Technology Extension Station of Gansu Province. Blood samples were collected from 117 Alpine Merino sheep with production records. 5 mL of blood was collected from the veins of each sheep and put into blood collection tubes added with EDTA-K2 anticoagulant. After the blood samples were collected, they were mixed quickly , placed in a sampling box containing ice packs for temporary storage, and then transported back to the laboratory and stored in a -20°C refrigerator for DNA extraction. The record of each wool length is provided by Gansu Sheep Breeding Technology Extension Station.
[0038] 2. Main reagents and instruments
[0039] EDTA-K2 vacuum blood collection tubes were purchased from Jiangsu Yuli Medical Instrument Co., Ltd.; blood genome extraction kit was purchased from Tiangen Biochemical T...
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