SNP (Single Nucleotide Polymorphism) molecular marker for influencing wool length character of alpine merino and application of SNP molecular marker

A molecular marker and Merino sheep technology, applied in the field of molecular genetics, can solve the problems that the molecular marker system of fine-haired wool traits has not been established, and important genetic resources have not been effectively excavated and utilized, so as to achieve easy interpretation of results and improve The effect of breeding accuracy and low cost

Active Publication Date: 2022-03-22
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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Problems solved by technology

In recent years, fine-wool sheep genetics and breeding experts at home and abroad have been devoting themselves to the research of candidate genes or molecular markers controlling wool traits, but no molecula

Method used

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  • SNP (Single Nucleotide Polymorphism) molecular marker for influencing wool length character of alpine merino and application of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker for influencing wool length character of alpine merino and application of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker for influencing wool length character of alpine merino and application of SNP molecular marker

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Embodiment 1

[0035] Example 1 Correlation Between Different Genotypes and Hair Length of Alpine Merino Sheep

[0036] 1. Sample Collection

[0037] The samples came from the Sheep Reproduction Technology Extension Station of Gansu Province. Blood samples were collected from 117 Alpine Merino sheep with production records. 5 mL of blood was collected from the veins of each sheep and put into blood collection tubes added with EDTA-K2 anticoagulant. After the blood samples were collected, they were mixed quickly , placed in a sampling box containing ice packs for temporary storage, and then transported back to the laboratory and stored in a -20°C refrigerator for DNA extraction. The record of each wool length is provided by Gansu Sheep Breeding Technology Extension Station.

[0038] 2. Main reagents and instruments

[0039] EDTA-K2 vacuum blood collection tubes were purchased from Jiangsu Yuli Medical Instrument Co., Ltd.; blood genome extraction kit was purchased from Tiangen Biochemical T...

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Abstract

The invention belongs to the technical field of molecular genetics, and particularly relates to an SNP molecular marker influencing the wool length character of alpine merino and application. The SNP molecular marker is located at the 24178878th nucleotide site T/C mutation on the 22nd chromosome of the international sheep reference genome Oarv4.0 version. The invention further relates to a specific primer pair for detecting the SNP molecular marker by using a PCR technology, a kit containing the primer pair and a nucleotide polymorphism detection method, and compared with a traditional detection method, the technology has the advantages of high accuracy, high detection speed, low cost, easiness in result judgment and the like. SNP locus detection is used for carrying out early selection of the high-mountain merino wool length character, shortening the cultivation period and accelerating the cultivation process, an early selection technology of the high-mountain merino wool length character is established, the breeding time of the high-mountain merino wool length character is shortened, the breeding cost is reduced, and the application value is very high.

Description

technical field [0001] The invention belongs to the technical field of molecular genetics, and in particular relates to a SNP molecular marker affecting the long traits of alpine merino wool and its application. Background technique [0002] Wool refers to a layer of fibers with textile value covering the surface of sheep, which is a derivative of skin. Wool is the main raw material of the wool spinning industry, accounting for about 97% of wool spinning raw materials, and is mainly used to process clothing fabrics, wool, blankets and other products. In wool production and breeding practice, wool traits are important economic traits, mainly including multiple indicators such as wool length, wool yield, average fiber diameter, curvature, breaking strength, elongation, net wool rate, etc., which are related to weaving products and Economic benefits are closely related. Demand for wool has outstripped supply due to population growth. As market demands change, producing high-...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 袁超卢曾奎郭婷婷杨博辉李建烨刘建斌岳耀敬牛春娥孙晓萍
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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