Reagent and kit for detecting 13 CODIS STR gene loci of trace mixed human source DNA sample and application thereof

A locus and kit technology, which is applied in the field of amplification and detection of human short tandem repeat sequences, can solve the problems of inability to distinguish genotypes, high requirements for detection of DNA sample abundance, etc., and achieve the effect of improving the detection limit.
CN107557481AInactive Publication Date: 2018-01-09SUZHOU GENO TRUTH BIOTECHNOLOGY CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SUZHOU GENO TRUTH BIOTECHNOLOGY CO LTD
Publication Date
2018-01-09
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a reagent and a kit for detecting 13 CODIS STR gene loci of a trace mixed human source DNA sample and an application thereof. The reagent comprises amplification primers for 13CODIS STR gene loci and a sex gene locus, wherein the 13 CODIS STR gene loci are separately TPOX, DS31358, FGA, D5S818, CSF1PO, D8S1179, D7S820, TH01, vWA, D13S17, D16S539, D18S51 and D21S11, and thesex gene locus is Amelogenin. The sequences of the amplification primers are separately shown as SEQ ID NO: 1-SEQ ID NO: 28. The 13 CODIS STR gene loci are independent of one another without a chaineffect, and can be matched with the gene loci of an existing commercial kit and a database of the Ministry of Public Security, so that the adaptability of an analytical result is achieved; meanwhile,a single DNA molecule can be amplified and detected; on the premise of ensuring the efficiency of the system, the detection rate of degraded / broken DNA templates can be increased; the reagent and kitcan be widely applied to the fields of individual recognition by legal medical experts, paternity test and other group genetics analysis.
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Description

Technical field

[0001] The invention relates to the amplification and detection of human short tandem repeats (STR), in particular to a reagent, kit and application for detecting 13 CODIS STR loci in a trace mixed human DNA sample, and belongs to the field of biotechnology. Background technique

[0002] Since the application of DNA atlas in forensic DNA analysis technology in 1985, DNA analysis technology has been widely used in forensic material evidence identification and has provided important evidence in many major criminal cases and civil disputes. At present, the multi-fluorescence-labeled composite amplification system based on capillary electrophoresis technology is the most widely used technology.

[0003] Short tandem repeats (STRs) are microsatellites and are widely distributed in the human genome. STR sites have high genetic diversity in the population, so they are often used as genetic markers. STR fragments are generally less than 500 bp and are Del’s law is codomina...

Claims

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