The invention relates to a quality detection method of a Jiuhua hemorrhoid suppository. The detection method disclosed by the invention comprises a method for identifying and determining rheum officinale, borneol, mangnolia officinalis and lithospermum, a method for determining the high performance liquid chromatography content of rheum officinale and a method for determining the gas chromatography content of borneol and isoborneol. The thin-layer identification method for rheum officinale, borneol, magnolia officinalis and lithospermum erythrorhizon comprises the following steps: taking jiuhua hemorrhoid suppository, grinding and adding methanol, dipping, filtering, evaporating filtrate to dryness, adding water into residues to dissolve, adding hydrochloric acid, heating, refluxing, immediately cooling, adding diethyl ether, shaking and extracting, merging diethyl ether liquid, recovering a solvent to dryness, adding methanol into residues to dissolve, and then taking the solution asa testing sample solution; taking a rhubarb reference medicinal material, preparing a reference medicinal material solution by the same method, taking a chrysophanol reference substance, and adding methanol to prepare a reference substance solution; and identifying according to the general rule 0502 in Chinese Pharmacopoeia 2015 edition by thin-layer chromatography.