Extraction method for hermetia illucens antibacterial peptide and application of hermetia illucens antibacterial peptide in preparation of fertilizer used for resisting tobacco diseases

A technology of soldier fly antibacterial peptide and extraction method, which is applied to the extraction of black soldier fly antibacterial peptide and the application field of fertilizers, can solve the problems of few black soldier fly antibacterial peptides and not being used to prepare tobacco disease-resistant fertilizers, and achieve improvement Agronomic traits and quality, good control effect, effect of improving economic value

Active Publication Date: 2020-06-26
松颉环保科技(深圳)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are few reports on the extraction and application of antimicrobial peptides from black soldier flies, and there is no report on the use of antimicrobial peptides from black soldier flies in the preparation of tobacco disease-resistant fertilizers

Method used

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  • Extraction method for hermetia illucens antibacterial peptide and application of hermetia illucens antibacterial peptide in preparation of fertilizer used for resisting tobacco diseases
  • Extraction method for hermetia illucens antibacterial peptide and application of hermetia illucens antibacterial peptide in preparation of fertilizer used for resisting tobacco diseases
  • Extraction method for hermetia illucens antibacterial peptide and application of hermetia illucens antibacterial peptide in preparation of fertilizer used for resisting tobacco diseases

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Embodiment 1

[0037] This embodiment provides a method for extracting black soldier fly antimicrobial peptides, comprising the following steps:

[0038] S1, preparation of R. solanacearum bacterium liquid: inoculate R. solanacearum bacteria in NA liquid medium (5g of peptone, 3g of beef extract, 1.5g of sodium chloride, 1L of deionized water), place at 30°C, 185r Cultivate in the dark for 54 hours under the condition of 1 / min, filter and dilute with sterilized water until the number of effective viable bacteria is 3.5×10 10 Bacterial solution of each / mL;

[0039] Preparation of tobacco wildfire bacteria liquid: Inoculate tobacco wildfire bacteria in NA liquid medium, culture in the dark at 28°C and 215r / min for 30h, filter and dilute with sterilized water until the number of effective viable bacteria is 3.5× 10 10 Bacterial solution of each / mL;

[0040] Preparation of Xanthomonas tabacum bacterial liquid: Inoculate Xanthomonas nicotiana into NA liquid medium, culture in the dark at 26°C ...

Embodiment 2

[0046] This embodiment provides a method for extracting black soldier fly antimicrobial peptides, comprising the following steps:

[0047] S1, preparation of R. solanacearum bacterium liquid: Inoculate R. solanacearum bacteria in NA liquid medium (5g of peptone, 3g of beef extract, 1.5g of sodium chloride, 1L of deionized water), place at 30°C, 170r Cultivate in the dark for 48 hours under the condition of 1 / min, and dilute with sterilized water after filtering until the number of effective viable bacteria is 2×10 10 Bacterial solution of each / mL;

[0048] Preparation of tobacco wildfire bacteria liquid: inoculate tobacco wildfire bacteria in NA liquid medium, culture in the dark at 28°C and 200r / min for 24 hours, filter and dilute with sterilized water until the number of effective viable bacteria is 2× 10 10 Bacterial solution of each / mL;

[0049] Preparation of Xanthomonas tabacum bacterial liquid: inoculate Xanthomonas nicotiana into NA liquid medium, culture at 26°C an...

Embodiment 3

[0055] This embodiment provides a method for extracting black soldier fly antimicrobial peptides, comprising the following steps:

[0056] S1, preparation of R. solanacearum bacterium liquid: Inoculate R. solanacearum bacteria in NA liquid medium (5g of peptone, 3g of beef extract, 1.5g of sodium chloride, 1L of deionized water), place at 30°C, 200r Cultivate in the dark for 60 hours under the condition of / min, and dilute with sterilized water after filtration until the number of effective viable bacteria is 5×10 10 Bacterial solution of each / mL;

[0057] Preparation of tobacco wildfire bacteria liquid: inoculate tobacco wildfire bacteria in NA liquid medium, culture at 28°C and 230r / min in the dark for 36h, filter and dilute with sterilized water until the number of effective viable bacteria is 5× 10 10 Bacterial solution of each / mL;

[0058] Preparation of Xanthomonas tabacum bacterial liquid: inoculate Xanthomonas nicotiana into NA liquid medium, culture at 26°C and 200...

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Abstract

The invention belongs to the field of fertilizer preparation, and specifically relates to an extraction method for hermetia illucens antibacterial peptide and an application of the hermetia illucens antibacterial peptide in preparation of a fertilizer used for resisting tobacco diseases. In order to prepare the fertilizer used for resisting the tobacco diseases from the hermetia illucens antibacterial peptide, a composite bacterial liquid composed of a tobacco ralstonia solanacearum bacterial liquid, a tobacco wildfire bacterial liquid and a tobacco angular leaf spot bacterial liquid is used as an induction liquid; hermetia illucens is induced to generate a large amount of antibacterial peptide under acupuncture soaking treatment; the hermetia illucens antibacterial peptide is prepared through an enzymolysis method; and the obtained hermetia illucens antibacterial peptide is high in content and high in activity and has good inhibiting effect on tobacco ralstonia solanacearum, tobacco wildfire pathogenic bacteria and tobacco angular leaf spot pathogenic bacteria. Meanwhile, a foliar fertilizer used for resisting the tobacco diseases is prepared from the hermetia illucens antibacterial peptide, and shows good prevention and treatment effects on tobacco bacterial angular leaf spot, tobacco bacterial wilt and tobacco wild fire. The agronomic traits and quality of the tobacco can beimproved, and the economic value of the tobacco is improved.

Description

technical field [0001] The invention belongs to the field of fertilizer preparation, and in particular relates to a method for extracting black soldier fly antibacterial peptide and its application in preparing fertilizer for resisting tobacco diseases. Background technique [0002] Tobacco (Nicotiana tabacum) belongs to Solanaceae (Solanaceae) Nicotiana (Nicotiana), is an annual herb. my country is a big tobacco producing country in the world today, and the total output and sales of tobacco leaves account for about 30% of the world. Tobacco is an important economic plant in my country, and tobacco tax accounts for 1 / 10 of my country's fiscal revenue. Tobacco is one of the crops most affected by diseases, and there are many kinds of diseases and complex symptoms. Common tobacco diseases include tobacco bacterial wilt, tobacco wildfire disease and tobacco angular leaf spot, etc. The hazards of tobacco diseases not only cause economic losses, but also cause great harm to the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C12N1/20C05G3/60C12R1/38C12R1/01
CPCC12P21/06C12N1/20C05G3/00C05B7/00C05D9/02C05F11/00
Inventor 孟杰
Owner 松颉环保科技(深圳)有限公司
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