Detection method of traditional Chinese medicine preparation Jiuhua hemorrhoid suppository

A technology of Jiuhua hemorrhoids and detection method, which is applied in the field of quality detection of traditional Chinese medicine preparations, and can solve the problems of not mentioning detection methods and the like.

Inactive Publication Date: 2020-03-27
JIANGXI JIUHUA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In the prior art, the patent number 201010590133.0, the applicant: Jiangxi Jiuhua Pharmaceutical Co., ...

Method used

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  • Detection method of traditional Chinese medicine preparation Jiuhua hemorrhoid suppository
  • Detection method of traditional Chinese medicine preparation Jiuhua hemorrhoid suppository
  • Detection method of traditional Chinese medicine preparation Jiuhua hemorrhoid suppository

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2、 9

[0150] Content determination of emodin and chrysophanol in embodiment 2, Jiuhua Zhichuang suppository

[0151] Chromatographic conditions: Ecosil, C18 chromatographic column, octadecylsilane bonded silica gel as filler; methanol-0.1% phosphoric acid solution (72:25) as mobile phase; flow rate 1.0ml / min, detection wavelength 254nm . The number of theoretical plates should not be less than 3000 based on the emodin peak.

[0152] Preparation of reference substance solution: Take appropriate amount of emodin reference substance and chrysophanol reference substance, weigh them accurately, add methanol to make a mixed solution containing 3 μg of emodin and 6 μg of chrysophanol per 1 ml.

[0153] Preparation of the test solution: Take the Jiuhua hemorrhoid suppository under the weight difference item, grind it, mix it evenly, take about 1 capsule, weigh it accurately, add 25ml of methanol accurately, weigh it, heat and reflux for 1 hour, take it out, Allow to cool, weigh again, mak...

Embodiment 3

[0156] Content determination of borneol and isoborneol in embodiment 3, Jiuhua Zhichuang suppository

[0157] Chromatographic conditions and system suitability test: capillary column with polyethylene glycol (PEG-20M) as stationary phase (column length is 30m, inner diameter is 0.32mm, film thickness is 0.5μm); column temperature is 120°C; sample injection The port temperature was 170°C, and the detector temperature was 220°C; the split injection was performed with a split ratio of 10:1. The number of theoretical plates should not be less than 5000 based on the borneol peak.

[0158] Determination of correction factor: Take an appropriate amount of methyl salicylate reference substance, weigh it accurately, add ethyl acetate to make a solution containing 0.25 mg per 1 ml, and use it as an internal standard solution. Take an appropriate amount of borneol reference substance and isoborneol reference substance respectively, weigh them accurately, add internal standard solution t...

Embodiment 4、 9

[0161] Embodiment 4, prescription and preparation method of Jiuhua hemorrhoid suppository:

[0162] Rhubarb 97g Fritillaria 97g Arborvitae leaves (fried) 276g Magnolia officinalis 276g

[0163] Baiji 138g, borneol 8.3g, comfrey 276g

[0164] Preparation method: the above seven flavors, except that borneol is crushed into fine powder for later use, the other six flavors are extracted with 70% ethanol under reflux for 3 times, the first time is 3 hours, the second time is 2 hours, the third time is 1.5 hours, the extracts are combined and recovered Ethanol, concentrated, the extract for later use.

[0165] Weigh 1200g of polyethylene glycol 6000 and 745g of polyethylene glycol 4000, heat and melt, mix well, add concentrated liquid medicine, stir well, cool to 50-55°C, add borneol fine powder and mix well to make 1000 cases (Each capsule weighs 2.1g), ready to use; or weigh 592g of polyethylene glycol 6000 and 953g of polyethylene glycol 4000, heat and melt, mix well, add conce...

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Abstract

The invention relates to a quality detection method of a Jiuhua hemorrhoid suppository. The detection method disclosed by the invention comprises a method for identifying and determining rheum officinale, borneol, mangnolia officinalis and lithospermum, a method for determining the high performance liquid chromatography content of rheum officinale and a method for determining the gas chromatography content of borneol and isoborneol. The thin-layer identification method for rheum officinale, borneol, magnolia officinalis and lithospermum erythrorhizon comprises the following steps: taking jiuhua hemorrhoid suppository, grinding and adding methanol, dipping, filtering, evaporating filtrate to dryness, adding water into residues to dissolve, adding hydrochloric acid, heating, refluxing, immediately cooling, adding diethyl ether, shaking and extracting, merging diethyl ether liquid, recovering a solvent to dryness, adding methanol into residues to dissolve, and then taking the solution asa testing sample solution; taking a rhubarb reference medicinal material, preparing a reference medicinal material solution by the same method, taking a chrysophanol reference substance, and adding methanol to prepare a reference substance solution; and identifying according to the general rule 0502 in Chinese Pharmacopoeia 2015 edition by thin-layer chromatography.

Description

technical field [0001] The invention relates to a quality detection method of traditional Chinese medicine preparations, in particular to a quality detection method of Jiuhua hemorrhoid suppository. Background technique [0002] Hemorrhoids mainly refer to the varicose veins formed by the enlarged varicose plexus under the rectal mucosa and under the skin of the anal canal. They are called hemorrhoids, hemorrhoids or hemorrhoids in medicine, but common people often call them hemorrhoids. It can occur at any age, but the incidence rate increases with age. The main clinical manifestations are prolapse, bleeding, anal discomfort and other symptoms. The sores in hemorrhoids mentioned by the common people mean sores, but hemorrhoids are not an infectious disease. Hemorrhoids are a venous mass disease and a vascular disease, so the treatment has its own particularity. It is often said that ten men have nine hemorrhoids or ten men have nine hemorrhoids. This sentence means that t...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/8679G01N30/90G01N2030/045
Inventor 杨华陈邦辉谢明艳孙新平刘建春许焰辉
Owner JIANGXI JIUHUA PHARMA
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