Multi-component comprehensively quantificational method for assessing and controlling quality of traditional Chinese medicine and application

A quality evaluation, traditional Chinese medicine technology, applied in the direction of measuring devices, material separation, analysis of materials, etc., can solve the problems of rough titer determination, inability to specifically reflect the laxative intensity of the ingredients that cause diarrhea

Active Publication Date: 2015-09-09
302 MILITARY HOSPITAL OF CHINA +1
View PDF4 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although literature [9, 10] involves the determination of laxative potency based on the mouse model of compound diphenoxylate constipation, the literature uses the crude extract of rhubarb decoction for

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multi-component comprehensively quantificational method for assessing and controlling quality of traditional Chinese medicine and application
  • Multi-component comprehensively quantificational method for assessing and controlling quality of traditional Chinese medicine and application
  • Multi-component comprehensively quantificational method for assessing and controlling quality of traditional Chinese medicine and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0115] Embodiment 1 (measurement of content of 10 kinds of active ingredients such as aloe-emodin-8-o-β-D-glucoside in rhubarb medicinal materials)

[0116] Test product: rhubarb test product (that is, the sample of rhubarb medicinal material to be tested)

[0117] Reference substance: aloe-emodin-8-o-β-D-glucoside (batch number 141225, used for content determination as 98.78), rhein-8-o-β-D-glucoside (batch number 141211, for content determination 98.57%), chrysophanol-8-o-β-D-glucoside (batch number 141220, used for content determination in 99.42%), emodin-8-o-β-D-glucoside (batch number 141209 , used for content determination at 98.67%), emodin methyl ether-8-o-β-D-glucoside (batch number 141012, used for content determination at 99.14%), provided by Chengdu Croma Biotechnology Company;

[0118] Aloe-emodin (batch number 110795-201007, for content determination at 98.05%), rhein (batch number 110757-200206, for content determination at 98.65%), emodin (batch number 110756,...

Embodiment 2

[0134] Embodiment 2 (the content determination of sennoside A, sennoside B in rhubarb medicinal material)

[0135] Test product: rhubarb test product (that is, the sample of rhubarb medicinal material to be tested)

[0136] Reference substances: Sennoside A (batch number 13122701, for content determination at 98.58%), sennoside B (batch number 13062606, for content determination at 98.35%), provided by Chengdu Purui Biotechnology Company;

[0137] Waters Acquity ultra-high performance liquid chromatography (Waters, USA), equipped with Waters PDA Detector (diode array detector), automatic sampler, Empower 2 chromatographic workstation, Acquity UPLC BEH C18 column (1.7μm, 2.1 × 100mm ), XS-205 electronic balance (METTLER TOLEDO), AL-204 electronic balance (METTLER TOLEDO), ultrasonic instrument (Nanjing Xinchen Biotechnology Co., Ltd., 40KHz).

[0138] Preparation of the reference solution of sennoside A and sennoside B:

[0139] with 0.1% NaHCO 3 45% methanol aqueous solution ...

Embodiment 3

[0145] Embodiment 3 (the detailed investigation of the mensuration of rhubarb-induced laxative effect component potency and the calculation of equivalence coefficient)

[0146] 140 ICR male mice, weighing 20g, were divided into 14 groups, 10 in each group. They were fasted for 12 hours before the experiment, and the first group was regarded as the normal group, without any modeling and administration; the second group As a model control group, only the compound difenoxate tablet was given to cause a constipation model; the remaining 12 groups were administered corresponding to 12 effect components. First use compound diphenoxylate tablet (dose 60mg·kg -1 ) for modeling by intragastric administration, and intragastric administration after 1 hour of modeling, with a dosage of 0.4ml (wherein the concentrations of 12 active ingredients such as aloe-emodin-8-o-β-D-glucoside and sennoside A are 1 mg / ml, dissolved in hot water at 37°C), after administration, fasting without water, a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Wavelengthaaaaaaaaaa
Login to view more

Abstract

The application of the invention provides a multi-component comprehensively quantificational method for assessing and controlling the quality of traditional Chinese medicine and application. The method for assessing and controlling the quality of traditional Chinese medicine combines measurement of chemical content and measurement of biological potency, focuses on comprehensively quantificational assessment on the quality of the rheum palmatum herbs or medicinal slices through biological potency, and establishes the equivalent computation method for components causing the rheum palmatum purgative effect; the method can be applied to preliminary assessment on the quality of rheum palmatum herbs or medicinal slices with different producing areas, different batches and different growth years.

Description

technical field [0001] This application relates to a method for evaluating the quality of traditional Chinese medicines with multi-component comprehensive quantification. Background technique [0002] Quality evaluation of traditional Chinese medicine has always been a hot and difficult point in the research and production of traditional Chinese medicine, and it is also an important basis and key to the modernization of traditional Chinese medicine. At present, the quality evaluation of traditional Chinese medicine mostly refers to the mode of phytochemistry and western medicine quality evaluation, and the quality of medicinal materials is evaluated by single determination of the content of certain chemical components in medicinal materials. From the perspective of traditional Chinese medicine, the control of a single component is difficult to truly reflect the efficacy of traditional Chinese medicine, especially for compound preparations. The detection of any index componen...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/88G01N33/15
Inventor 肖小河王伽伯谭鹏张定堃李刚严桂林
Owner 302 MILITARY HOSPITAL OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap