44results about How to "Strong Raman signal" patented technology

The present invention relates to a microfluidic chip, in particular, it relates to a microfluidic chip with surface enhanced Raman spectrum (SERS) active substrate and its preparation method. On the microfluidic chip, at least a microchannel is set, on the total or partial inner wall surface of said microchannel is set a layer of rough metal film with SERS activity. Besides, said invention also provides the concrete steps for preparing said microfluidic chip.

The invention belongs to the field of analytical chemistry and relates to a method for building a surface enhanced Raman scattering detection system. The method comprises the following steps: firstly, embedding the aptamer sequences of a target object into six DNA (deoxyribonucleic acid) edges of a nanosilver assembly body; secondly, shortening the distance between nanosilver particles through specific identification of the target object and the aptamers of the target object; thirdly, forming a high-density electrical magnetic field region between the silver particles within the very short distance; fourthly, enhancing Raman signals of beacon molecules in the region; finally, detecting the target object through the change of the Raman signals of the beacon molecules. According to the method, the target object, especially kanamycin can be specifically, quickly and ultra-sensitively detected.

The invention discloses a three-dimensional metal-graphene composite substrate and a preparation method thereof. The composite substrate disclosed by the invention is composed of a first nano layer, a graphene layer and a second nano layer, wherein the first nano layer is deposited on the surface of an amorphous carbon underlayer, the graphene layer is arranged on the surface of the first nano layer through spin coating, the second nano layer is deposited on the surface of the graphene layer, and the first nano layer and the second nano layer are gold or silver nano layers. According to the method, an active substrate with a good surface enhanced Raman effect is constructed; and the three-dimensional metal-graphene composite substrate is simple in preparation method, high in efficiency, and low in preparation cost.

The invention relates to a metal surface enhanced Raman scattering substrate with a three-dimensional period structure and a preparation method thereof and belongs to a chemical detection technology. The preparation method comprises the following steps of: soaking an amine-modified bio-template into a chloroauric acid solution so that catalyzed metal ions are introduced; then carrying out chemical plating treatment on activated sodium borohydride to obtain a metalized bio-template; and finally, carrying the metal ions on the metalized bio-template so as to obtain the metal surface enhanced Raman scattering substrate. The metal surface enhanced Raman scattering substrate prepared by the preparation method, provided by the invention, carries on and copies the three-dimensional period structure of a natural organism. The technology is simple and flexible and the cost is low. The obtained three-dimensional period metal substrate has higher surface enhanced Raman effects, can be used for effectively enhancing Raman signals, and has the advantages of uniform signal response and good repeatability.

The invention discloses a preparation method of a carbon-based quantum dot / nano-silver surface enhanced raman base. The preparation method comprises the following steps: dissolving carbon-based quantum dots and silver ions in a water solution; adding sodium boro-hydride serving as a reducing agent under a stirring state, so as to prepare a carbon-based quantum dot / nano-silver composite material. The preparation method is simple, convenient, easy, free of pollution, fast in reaction, and high in operability. As the carbon-based quantum dots on the surface of the carbon-based quantum dot / nano-silver composite material comprise a large quantity of oxygen-containing functional groups, the carbon-based quantum dot / nano-silver composite material is favorable in dispersity and long-lasting in stability in water. More importantly, as the carbon-based quantum dot / nano-silver composite material comprises a large quantity of 'notches', the surface enhanced raman activity is excellent; as the carbon material on the surface of the carbon-based quantum dot / nano-silver composite material can adsorb benzene series via electrostatic adsorption and Pi-Pi function, the carbon-based quantum dot / nano-silver surface enhanced raman base can be applied to surface enhanced raman detection of the benzene series.

The invention belongs to the technical field of digital microfluidics, and particularly relates to a target detecting method based on a digital microfluidic technology and a surface-enhancement Ramanscattering technology. The system mainly comprises a digital microfluidic chip and a surface-enhancement Raman probe. The digital microfluidic chip consists of an upper polar plate and a lower polar plate. The surface-enhancement Raman probe comprises a metal core, a Raman report molecule and a shell layer, and the Raman report molecule is embedded between core-shell structures. By the method automated control on discrete droplets on an electrode array is implemented based on a dielectric wetting principle, a reaction system on a chip is constructed, and Raman signals are output rapidly in real time. The target detecting method has the advantages of full automatic processing, simplicity and rapidness, high sensitivity, suitability to a complicated biosystems and the like. A plurality of samples are simultaneously detected in parallel by program control; and the target detecting method can be widely applied to detection of various types of targets, and particularly can be used for detecting rare samples and infectious samples.

A method for preparing a transparent Raman effect film relates to preparation of optical fiber films and aims to solve the problems of complicated equipment and difficulty in controlling a production process of an existing method for preparing SERS (surface enhanced Raman scattering) base materials. The method includes the steps: firstly, preparing cellulose acetate solution with acetone, acetic acid and cellulose acetate; secondly, uniformly mixing Ag NPs suspending liquid and the cellulose acetate solution to obtain spinning liquid; thirdly, spinning the spinning liquid into a nano-fiber nonwoven felt by means of electrostatic spinning process; and fourthly, tiling the cut nano-fiber nonwoven felt in a slot of a composite film forming tool, pouring polyvinyl alcohol water solution into the slot, leading the polyvinyl alcohol water solution to be lower than the upper edge of the slot of the composite film forming tool, and maintaining pressure and drying to obtain the transparent Raman effect film. The method is mainly used for preparing the transparent Raman effect film.

The invention discloses a method for preparing a surface-enhanced Raman spectroscopy substrate of a silver self-assembly under the assistance of amino acids, relating to the preparation method of the surface-enhanced Raman spectroscopy substrate and aiming at solving the problems of poor uniformity of signals, low sensitivity, complex preparation process and high cost of a nano-silver surface-enhanced Raman spectroscopy substrate which is prepared by using the prior art. The method for preparing the surface-enhanced Raman substrate comprises the following steps of: injecting little amount of amino acids into a silver nitrate solution under an ice-water bath condition, cooling for a period of time, and then adding ascorbic acid with a certain concentration and reacting to obtain the surface-enhanced Raman spectroscopy substrate of the silver self-assembly with a certain three-dimensional structure. The surface-enhanced Raman spectroscopy substrate can be used for detecting organic molecules and biological molecules of ppm-grade concentration and has the advantages of uniform signal response, high sensitivity, simple and rapid method and low cost. The method disclosed by the invention is applied to the detection field of organic molecules and biological molecules.

The invention discloses application of polymer microspheres to Raman detection. Methacrylate / amide polymer monomers or styrene polymer monomers containing alkynyl, cyano, azido or a carbon-deuterium bond group are prepared into the polymer microspheres with the particle diameter from a nanometer grade to a micron grade through an emulsion polymerization or dispersion polymerization method; the polymer microspheres have a remarkable Raman signal under the condition of no metal sensitization structure; and a Raman characteristic peak signal is located in a Raman quiet zone (1800cm<-1> to 2800cm<-1>) in a living organism and can be used as a marker for biological imaging.

An assay method and kit for detecting a chemical. The method and kit utilize a metal surface capable of surface enhanced Raman Scattering. The metal surface may be provided in the form of one or more nanoparticles, to increase the surface enhanced Raman Scattering capability of the metal surface. The nanoparticles may be treated with one or more additives to further enhance or maintain the surface enhanced Raman Scattering capability of the nanoparticles.

The invention relates to a temperature-sensitive intelligent response SERS substrate and a preparation method and application thereof. The preparation method comprises the following steps of taking azodiisobutyronitrile as an initiator, methanol as a solvent and 1-vinyl-3-ethylimidazolium bromide and N-isopropylacrylamide as monomers to carry out copolymerization reaction;re-dispersing the copolymer into the deionized water after precipitation, filtering and drying; adding an excessive amount of a tetrachloroauric acid solution, carrying out an ion exchange reaction, removing the unreacted tetrachloroauric acid, carrying out in-situ reduction by using sodium borohydride to generate poly (NIPAm-co-ViEtImBr)-AuNPs, continuously immobilizing probe molecules by using an ion exchange effect, and carrying out a Raman signal test. According to the invention, the intelligent polymer PNIPAM with temperature-sensitive response, the gold nanoparticles with enhancement effect on Raman signals andthe probe molecules are successfully integrated through a bridging effect of the ionic liquid.

The invention discloses a method of assembling a nano necklace based on DNA nanoribbon templating and gold particles; the method comprises: synthesizing gold nano particles, mixing DNA framework chains having molar concentration of 1-5 Mumol / L and staple single chains having molar concentration of 1-5 Mumol / L according to a size ratio of 1:1 to 1:5, adding gold nano particles, stirring and mixing well, and performing gradient annealing on the solution of step C at 45-25 DEG C to form nano necklace structure. Gold particles 20 nm in size and DNA nanoribbons specifically designed are assembled via one step to form the nano gold chain, nano gold is modified via 4-mba Raman signal molecules, and its Raman signal changes are detected. The method has the advantages of good DNA nanoribbon structure, high forming yield, low external environment requirements and the like; 4-mba signal molecules have great Raman signals, modifying the nano necklace so that the necklace has better advantages in Raman detection; the nano necklace is characterized by the aid of an atomic force microscope and a transmission electron microscope, and the necklace also has the advantages of good simplicity, good reliability, low cost and the like.

The invention discloses a preparation method of a nano tin dioxide / carbon basis point / nano silver surface-enhanced Raman substrate. Nano tin dioxide / carbon basis point nanomaterial and nano silver aredissolved in water, weak aqua ammonia is added under the condition of stirring to adjust pH to be alkalescent and then a reducing agent glucose is further added, and thus a nano tin dioxide / carbon basis point / nano silver composite material can be prepared. The preparation method provided by the invention is simple, convenient and pollution-free, and has a swift response and extremely strong operability. Carbon basis points on the surface of the acquired nano tin dioxide / carbon basis point / nano silver composite material have a lot of oxygen-containing functional groups, so that the compositematerial has good dispersibility in water. The composite material serving as the more excellent surface-enhanced Raman substrate has the electromagnetic enhancement effect included by noble metal nano particles, and the composite semiconductor material has the certain chemical enhancement effect, and meanwhile the carbon material on the surface of the composite material can adsorb benzene seriesvia electrostatic adsorption and pi-pi interaction, so that the composite material can be applied to surface-enhanced Raman detection.

The invention discloses an antibiotic Raman spectrum detection method based on a surface-modified magnetic nano-silver flower substrate. The detection method comprises the following steps: coating Fe3O4 nanoparticles of 200nm with a SiO2 layer, generating a large amount of silver seeds on the surface of the SiO2 shell, and breeding a flower-shaped structure by taking seeds as a core; connecting 1-hexanethiol with Ag shell through a sulfhydryl group, and enriching antibiotics by using a surface self-organization hydrophobic layer; diluting the two kinds of antibiotics respectively with methanol water and milk to different concentrations, and purifying and enriching with a SPE (Solid-Phase Extraction) column; acquiring lowest detection limits of the antibiotics in different substances through the Raman spectrum detection, wherein a standard recovery rate is between 78.50 and 113.59 percent. The method is high in recovery rate, and can satisfy the requirement on antibiotics residue analysis. The 1-hexanethiol-modified magnetic nano-silver flower particles prepared by the invention are taken as a high-performance the SERS (Surface Enhanced Raman Scattering) substrate, are provided with a large quantity of hot spots, and can be taken as simple, rapid, high-sensitivity antibiotics residue analysis method.

The SERS virus detection chip for detecting the novel coronavirus comprises an SERS substrate, graphene arranged on the SERS substrate and an ACE2 biological probe arranged on the graphene, and the SERS substrate comprises a Si / SiO2 wafer serving as a base and a metal nanostructure above the Si / SiO2 wafer; the preparation method comprises the following specific steps: (1) preparing a metal nanostructure on a Si / SiO2 wafer to form an SERS substrate; (2) transferring the single-layer CVD graphene to an SERS (Surface Enhanced Raman Scattering) substrate; and (3) modifying ACE2 on graphene to form a new crown detection probe, thereby completing the preparation of the SERS virus detection chip. The ACE2 biological probe molecules and the graphene / periodic nano-metal structure SERS detection substrate are combined to form the new coronavirus biological detection chip, the detection chip is utilized to achieve high-sensitivity detection of the new coronavirus, the detection is rapid and efficient, and the capturing efficiency is higher.

A method for preparing a transparent Raman effect film relates to preparation of optical fiber films and aims to solve the problems of complicated equipment and difficulty in controlling a production process of an existing method for preparing SERS (surface enhanced Raman scattering) base materials. The method includes the steps: firstly, preparing cellulose acetate solution with acetone, acetic acid and cellulose acetate; secondly, uniformly mixing Ag NPs suspending liquid and the cellulose acetate solution to obtain spinning liquid; thirdly, spinning the spinning liquid into a nano-fiber nonwoven felt by means of electrostatic spinning process; and fourthly, tiling the cut nano-fiber nonwoven felt in a slot of a composite film forming tool, pouring polyvinyl alcohol water solution into the slot, leading the polyvinyl alcohol water solution to be lower than the upper edge of the slot of the composite film forming tool, and maintaining pressure and drying to obtain the transparent Raman effect film. The method is mainly used for preparing the transparent Raman effect film.

The invention relates to a preparation method and an application of a raman enhancement substrate based on a combination of a club-shaped upconversion material (UC) and an Au@Ag nanomaterial. A principle of the substrate is to use a characteristic that upconversion can absorb visible light emitted by near infrared light, so as to convert the near infrared light to visible light at a local plasma resonance frequency part of an Au@Ag nanoparticle; the local plasma resonance frequency of the Au@Ag nanoparticle is improved; and further, raman signal intensity is improved. The preparation method of the enhancement substrate particularly comprises the steps of preparation of the club-shaped upconversion material (UC), preparation of upconversion material surface modified silicon dioxide (UC@SiO2), preparation of the Au@Ag nanoparticle and assembly (UC@SiO2@Au@Ag) of UC@SiO2 and Au@Ag nanoparticle. The substrate has the maximum advantage that the substrate has a better enhancement effect that a substrate without the upconversion under the same condition, and the signal intensity is improved approximately by 11 times. The invention provides a raman spectrum detection method by taking UC@SiO2@Au@Ag as the substrate. Compared with the traditional raman substrate, the substrate has the very good enhancement effect and very wide application prospects.

A surface enhanced raman spectroscopy substrate of continuous three dimensional structural nano silver and a preparation method thereof relate to a surface enhanced raman spectroscopy substrate of nano silver and a preparation method thereof. The invention solves the problems of the surface enhanced raman spectroscopy substrate of nano silver prepared by the prior art, such as poor signal uniformity, low sensitivity, complicated preparation technique and high cost. The substrate consists of a polyaniline film, a nano gold layer and a three dimensional nano silver layer sequentially from bottomto top. The preparation method of the substrate comprises the steps of: dissolving undoped polyaniline powder in N-methylpyrrolidone to form homogeneous solution, spreading the solution on a glass substrate, conducting curing to obtain the polyaniline film; later dipping the polyaniline film in gold-bearing solution to obtain the nano gold layer; and then dipping the polyaniline film coated withthe nano gold layer in silver nitrate soluation for reaction to obtain the surface enhanced raman spectroscopy substrate of the continuous three dimensional structural nano silver. The substrate can be used for detection of organic molecules and biological molecules with ppm concentration and has uniform signal response, high sensitivity, simple and fast method, and low cost.

The invention provides a compact 266nm short-wave ultraviolet Raman spectrometer which comprises a light source, an external light path, a dispersion system, a receiving system and a signal acquisition system. The light source is an all-solid-state 266nm short-wave ultraviolet laser; the external light path comprises a collimating lens, a first reflector, a dichroscope, a beam expander, an opticalfilter, a relay lens, a coupling optical fiber and a shaping optical fiber bundle, the dispersion system comprises a slit, a second reflector, an off-axis parabolic reflector and a plane reflection type grating, the receiving system is a back-illuminated CCD linear array detector, and the signal acquisition system is a computer. The optical fiber bundle is composed of a plurality of optical fibers, one end of the optical fiber bundle is arranged to be an optical fiber array, the other end of the optical fiber bundle is arranged to be linear, the point-to-line optical fiber bundle improves theluminous flux entering a dispersion system and increases the signal-to-noise ratio of the system, and in addition, the Raman spectrometer dispersion method based on the Littrow structure can effectively reduce spherical aberration and improve the resolution of the spectrometer.

The invention discloses a method for detecting ciprofloxacin in milk, and the method comprises the following steps: carrying out deproteinization operation on a milk sample to obtain a solution to bedetected; placing the surface enhancing reagent in a container; putting the to-be-detected liquid into a container; putting the flocculating agent into a container; uniformly mixing the solution in the container to obtain a mixed solution; performing Raman spectrum scanning on the mixed solution to obtain a Raman spectrum of the mixed solution, and determining whether ciprofloxacin exists in the milk or not based on the characteristic peak of the obtained Raman spectrum. According to the method, a surface enhancing reagent and a flocculating agent are adopted as SERS enhancing reagents, the SERS effect of ciprofloxacin in milk can be enhanced, stronger ciprofloxacin Raman signals are obtained, the detection sensitivity of ciprofloxacin in milk is greatly improved, and the lower detection limit of ciprofloxacin is low.

An assay method and kit for detecting a chemical. The method and kit utilize a metal surface capable of surface enhanced Raman Scattering. The metal surface may be provided in the form of one or more nanoparticles, to increase the surface enhanced Raman Scattering capability of the metal surface. The nanoparticles may be treated with one or more additives to further enhance or maintain the surface enhanced Raman Scattering capability of the nanoparticles.

The invention discloses a method for detecting benzoic acid in drink. The method comprises the steps of enriching benzoic acid in the drink through a silica gel extraction substrate, and detecting the benzoic acid in combination with surface-enhanced Raman spectroscopy. By combining the characteristics of high extraction efficiency, short extraction time and selective extraction of a target from a complicated substrate of a thin film microextraction technology with the advantages of convenience and quickness in detection and high sensitivity of the surface-enhanced Raman spectroscopy, a measuring method for the benzoic acid in the drink, which is easy to operate, high in sensitivity, low in detection cost, convenient and quick, is provided.

The invention discloses a Keemun black tea producing area identification method based on a Raman spectrum fingerprint technology, and belongs to the technical field of agricultural product and food producing area identification. The method for identifying the producing area of the Keemun black tea comprises the following steps: (1) preparing black tea soup; (2) uniformly mixing the black tea soup and the nano-silver sol, drying, and carrying out Raman detection to obtain original Raman spectrum data of the black tea powder; calculating the relative peak intensity; (3) respectively combining the relative peak intensity with stoichiometric method linear discriminant analysis, a K-nearest neighbor method and a random forest method to construct a discriminant model; (4) collecting Raman spectrum data of a to-be-detected sample according to the steps (1) and (2), and calculating to obtain relative peak intensity; and predicting the category of the sample by using the discrimination model in the step (3), and determining the production place of the to-be-detected sample. The discrimination model constructed by the method is high in overall correct recognition rate, and can effectively recognize and classify the Keemun black tea in small-producing areas and the Keemun black tea in large-producing areas.

The invention relates to a preparation method of a sweat sensing bandage based on SERS, and belongs to the field of composite materials. Based on the SERS method, electrolyte and metabolites (such as lactic acid and glucose concentration) in sweat of healthy people and local sweat loss of different parts of the body can be sampled and monitored. The sensing bandage prepared by the invention is designed by a traditional textile processing method on the basis of the original softness and wearability of fabrics and yarns, which provides a wearable sweat monitoring sensing platform which is simple to manufacture and good in applicability and usability, and a potential strategy is provided for instant diagnosis of diseases and health condition monitoring.

The invention discloses a detection method for detecting dioxin and polychlorinated biphenyl. The preparation method comprises the following steps: respectively preparing micro-nano silver particles,a molybdenum sulfide / graphene oxide composite material and magnesium-doped nano zinc oxide with a porous structure; superposing the micro-nano silver particles, the molybdenum sulfide / graphene oxide composite material and the magnesium-doped nano zinc oxide with the porous structure on the surface of the silicon substrate layer by layer to form a composite substrate with a self-cleaning function,and accurately and sensitively detecting dioxin and polychlorinated biphenyl by adopting a surface enhanced Raman spectroscopy technology on the basis of the composite substrate. Through the above mode, the method can effectively improve the intensity of Raman signals and enrich dioxin and polychlorinated biphenyl to be detected on the substrate by utilizing the synergistic effect of all layers ofmaterials on the substrate, so that the detection accuracy and sensitivity are improved; in addition, dioxin and polychlorinated biphenyl can be subjected to photodegradation after detection, self-cleaning of the substrate is achieved, frequent replacement of the substrate is avoided, and the detection cost is reduced; and the detection process is simple to operate, low in time consumption and low in cost, and has relatively high application value.

The invention discloses a method for detecting benzoic acid in drink. The method comprises the steps of enriching benzoic acid in the drink through a silica gel extraction substrate, and detecting the benzoic acid in combination with surface-enhanced Raman spectroscopy. By combining the characteristics of high extraction efficiency, short extraction time and selective extraction of a target from a complicated substrate of a thin film microextraction technology with the advantages of convenience and quickness in detection and high sensitivity of the surface-enhanced Raman spectroscopy, a measuring method for the benzoic acid in the drink, which is easy to operate, high in sensitivity, low in detection cost, convenient and quick, is provided.

The invention discloses Raman coding microspheres and a preparation method thereof, wherein the Raman coding microspheres are obtained by coating multi-layer metal nanoparticles on the surfaces of inner core nanoparticles through a layer-by-layer self-assembly method and marking with a Raman active substance. The preparation method and raw materials of the Raman coding microspheres are simple, and the strength of Raman signals of a marker can be regulated and controlled through the number of assembly layers of the metal nanoparticles; compared with the existing Raman making technology, the Raman coding microspheres according to the invention are strong in the Raman signals, uniform in aggregation state of the particles and good in repeatability of the Raman signals.

The invention discloses an MXene probe applied to a surface enhanced Raman spectroscopy (SERS) immune test. A technical method of the MXene probe comprises the following steps: (1) synthesizing an MXene two-dimensional material based on V2CTx; and (2) synthesizing an MXene (at) Thi (at) AuNPs two-dimensional plane Raman probe molecule, and modifying a recognition antibody (BindingAntibody) to obtain the MXene probe which can be applied to a surface enhanced Raman spectroscopy (SERS) immune test. And (3) modifying the antibody on the polystyrene ELISA plate to capture the target protein, and adsorbing the MXene probe specific recognition target on the ELISA plate. And (4) carrying out surface enhanced Raman spectroscopy (SERS) immunodetection by adopting a handheld Raman instrument. The MXene material is easy to prepare and modify, good in biocompatibility and capable of adsorbing a large number of small molecular substance thionine (Thi) with strong Raman signals and generating obvious Raman signals, and the polystyrene ELISA reaction plate serving as an SERS immunodetection substrate has the advantages of low preparation cost, uniform property, simple modification method and the like. The handheld Raman instrument is adopted for detection, and compared with traditional large-scale confocal Raman detection and traditional ELISA detection, portability can be achieved, and detection can be achieved anytime and anywhere.