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Method of assembling nano necklace based on DNA nanoribbon templating and gold particles

A technology of gold nanoparticles and gold particles, which is applied in the field of detection and analysis, can solve the problems that DNA nanobelts have not been used to assemble gold nanoparticles, and achieve the effects of precise space addressability, high formation yield, and simple structure

Inactive Publication Date: 2017-05-31
NANJING UNIV OF POSTS & TELECOMM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Feldmann (ühler, P., Roller, E.M., Schreiber, R., Liedl, T., Lohmüller, T., & Feldmann, J. Nano Letters, 2014(5), 2914-2919) and Finkelstein (Pilo-Pais, M .,Watson,A.,Demers,S.,Labean,T.H.,&Finkelstein,G.Nano Letters,2014(4),2099-2104.) Two scholars studied the surface Raman enhancement effect on DNA origami assembly in 2014, But to date, there have been no research and patent literature reports using DNA nanoribbons to assemble gold nanoparticles into nanostructures for Raman detection and others.

Method used

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  • Method of assembling nano necklace based on DNA nanoribbon templating and gold particles
  • Method of assembling nano necklace based on DNA nanoribbon templating and gold particles
  • Method of assembling nano necklace based on DNA nanoribbon templating and gold particles

Examples

Experimental program
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Effect test

Embodiment 1

[0047] For the convenience of those skilled in the art to further understand and implement the present invention, the following relevant examples are now provided: Embodiment 1: About how to prepare 20nm gold nanoparticles

[0048] (1) Place the chloroauric acid solution with a mass fraction of 1% in a three-necked flask with a lid, stir and boil in an oil bath (150° C.);

[0049] (2) To the solution in step (1), add sodium citrate with a molar concentration of 40mmol / L, boil and vigorously stir for 10min to make the solution evenly mixed;

[0050] (3) Turn off the heat source, stir vigorously for 15 minutes, the solution turns from yellow to red, and let stand until room temperature;

[0051] (4) After the reaction is finished, the solution obtained in step (3) is concentrated by centrifugal purification, and the centrifuged product is dispersed into ultrapure water.

Embodiment 2

[0053] Example 2: About the preparation of gold nanoparticles modified by ssDNA1

[0054] Take 150 μL of gold nanoparticle solution in Example 1 to prepare ssDNA1-modified gold nanoparticles:

[0055] (1) Wash 150 μL of the gold nanoparticle solution by centrifugation once, centrifuge at 9000 rpm for 10 minutes, and disperse the centrifuged product into 100 μL of ultrapure water;

[0056] (2) Add 5 μL of 100 μM ssDNA1 to the solution in step (1), and at the same time add 1.5 μL of 1% sodium dodecyl sulfate solution, oscillate and mix, and place at a temperature of 37°C and a rotation speed of 250rpm constant temperature shaker, shake and incubate for 4 hours;

[0057] (3) Add 2 mol / L sodium chloride solution dropwise to the solution in step (2), add 2.5 μL each time, and add dropwise 4 times at an interval of half an hour. A constant temperature shaker at 250rpm, shaking and incubating for 8h;

[0058] (4) After the reaction in step (3), the gold nanorods modified by the ss...

Embodiment 3

[0061] Embodiment 3: About the assembly method based on DNA nanobelt template gold particle

[0062] (1) The DNA backbone chain (nanoribbon-scafford) with a molar concentration of 4 μmol / L and the single-strand staple (nanoribbon-staple1, Modificatory nanoribbon-staple1, nanoribbon-staple2, nanoribbon-staple3, Modificatory nanoribbon-staple3) were mixed in equal volumes, 2 μL of each chain was taken, and 10 μL of 1×TAE-Mg buffer was added to make up to 20 μL, shaken and mixed. Then add 10 μL of the concentrated gold nanoparticle solution in Example 2, shake and mix.

[0063] (2) Place the mixed solution of step (1) in a PCR instrument and anneal at a rate of 0.1°C / 10s from 45°C to 25°C, take a newly dissociated mica sheet, drop 5 μL of the above-mentioned mixture after reaction, and dry it. The atomic force microscope was used for characterization, and the characterization results are shown in image 3 and Figure 4 .

[0064] From image 3 It can be seen that the yield o...

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Abstract

The invention discloses a method of assembling a nano necklace based on DNA nanoribbon templating and gold particles; the method comprises: synthesizing gold nano particles, mixing DNA framework chains having molar concentration of 1-5 Mumol / L and staple single chains having molar concentration of 1-5 Mumol / L according to a size ratio of 1:1 to 1:5, adding gold nano particles, stirring and mixing well, and performing gradient annealing on the solution of step C at 45-25 DEG C to form nano necklace structure. Gold particles 20 nm in size and DNA nanoribbons specifically designed are assembled via one step to form the nano gold chain, nano gold is modified via 4-mba Raman signal molecules, and its Raman signal changes are detected. The method has the advantages of good DNA nanoribbon structure, high forming yield, low external environment requirements and the like; 4-mba signal molecules have great Raman signals, modifying the nano necklace so that the necklace has better advantages in Raman detection; the nano necklace is characterized by the aid of an atomic force microscope and a transmission electron microscope, and the necklace also has the advantages of good simplicity, good reliability, low cost and the like.

Description

technical field [0001] The invention belongs to the field of detection and analysis, and in particular relates to a DNA nanobelt template gold particle one-step method for assembling a nano necklace and its preparation method and application. Background technique [0002] DNA nanotechnology originated from an idea proposed by American scientist Seeman in the 1980s: using the periodic regular structure formed by the base complementarity of DNA as the framework for the crystallization of other molecules such as proteins, which can provide an ideal for crystallography. research tools. In 2006, Rothemund further used the 7249nt genomic DNA of M13 bacteriophage as the main chain, and bent and "folded" it into various two-dimensional structures by designing 216 artificially synthesized short chains. This is the so-called "DNA origami". This technology has the advantages of simple process, easy operation and high yield, and has been widely used in biosensing, material detection, ...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 樊春海晁洁王旭汪联辉
Owner NANJING UNIV OF POSTS & TELECOMM
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