Method for building surface enhanced Raman scattering detection system

A technology for surface-enhanced Raman and construction methods, applied in the field of analytical chemistry to improve specificity and sensitivity, reduce false positives, and avoid interference

Active Publication Date: 2014-12-10
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Currently, few nanoparticle assemblies are used for the Raman detection of kanamycin.

Method used

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  • Method for building surface enhanced Raman scattering detection system
  • Method for building surface enhanced Raman scattering detection system
  • Method for building surface enhanced Raman scattering detection system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Synthesis of silver nanoparticles

[0044] The preparation method of silver nanoparticles: get a clean Erlenmeyer flask and place it in an ice bath, add 20mL ultrapure water, 5mL 1% polyvinylpyrrolidone (PVP), 0.5mL 0.01mol / L sodium borohydride and 0.5mL1 % trisodium citrate in water. Then, use two 50mL needle tubes to fill 5mL 1% polyvinylpyrrolidone aqueous solution and 5mL 0.1% silver nitrate aqueous solution respectively. To the Erlenmeyer flask in the ice bath, add it while stirring until the end of the reaction, the solution turns from colorless to dark yellow, put the reactant at 80°C for 2 hours to remove unreacted sodium borohydride, and finally the solution turns bright yellow, The transmission electron microscope shows that the particle size is 20±3nm, such as figure 1 shown.

[0045] (2) Silver nanoparticles surface modified DNA

[0046] Centrifuge 200uL of silver nanoparticles prepared in step (1) at 7500r / min for 15min, add 15mM tris-hydrochloric a...

Embodiment 2

[0052] (1) Synthesis of silver nanoparticles

[0053] The preparation method of silver nanoparticles: get a clean Erlenmeyer flask and place it in an ice bath, add 20mL ultrapure water successively, 5mL 1% polyvinylpyrrolidone (PVP) and 0.5mL 0.01mol / L sodium borohydride and 0.5mL1 % trisodium citrate in water. Then, use two 50mL needle tubes to fill 5mL 1% polyvinylpyrrolidone aqueous solution and 5mL 0.1% silver nitrate aqueous solution respectively. To the Erlenmeyer flask in the ice bath, add it while stirring until the end of the reaction, the solution turns from colorless to dark yellow, put the reactant at 80°C for 2 hours to remove unreacted sodium borohydride, and finally the solution turns bright yellow, The transmission electron microscope shows that the particle size is 20±3nm, such as figure 1 shown.

[0054] (2) Silver nanoparticles surface modified DNA

[0055] Centrifuge the silver nanoparticles prepared in step (1) at 8000r / min for 10min, add 10mM tris-hyd...

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Abstract

The invention belongs to the field of analytical chemistry and relates to a method for building a surface enhanced Raman scattering detection system. The method comprises the following steps: firstly, embedding the aptamer sequences of a target object into six DNA (deoxyribonucleic acid) edges of a nanosilver assembly body; secondly, shortening the distance between nanosilver particles through specific identification of the target object and the aptamers of the target object; thirdly, forming a high-density electrical magnetic field region between the silver particles within the very short distance; fourthly, enhancing Raman signals of beacon molecules in the region; finally, detecting the target object through the change of the Raman signals of the beacon molecules. According to the method, the target object, especially kanamycin can be specifically, quickly and ultra-sensitively detected.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to a method for constructing a surface-enhanced Raman scattering detection system. Background technique [0002] The Raman effect, also known as Raman scattering, is an inelastic scattering phenomenon of photons. When light is scattered from an atom or molecule, most of the photons are elastically scattered, which is called Rayleigh scattering. Under Rayleigh scattering, the scattered photons have the same energy, frequency and wavelength as the incoming photons. However, a small fraction of scattered photons (approximately 1 in 10 million) will change in frequency after scattering, usually lower than the incident photon frequency, due to the interaction between the incident photon and the medium molecules. energy exchange. This is Raman scattering. Raman spectrum is a kind of scattering spectrum. Raman spectroscopy is based on the Raman scattering effect discovered by Indian s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 严文静章建浩
Owner NANJING AGRICULTURAL UNIVERSITY
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