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Target detecting method and system based on digital microfluidic technology and surface-enhancement Raman scattering technology

A technology of surface-enhanced Raman and digital microfluidics, which is applied in Raman scattering, chemical instruments and methods, fluid controllers, etc., can solve the problems of increased detection costs, large differences in instability analysis results, etc., and reduce detection The effect of reducing cost, shortening detection time, and avoiding secondary infection

Inactive Publication Date: 2018-05-25
XIAMEN UNIV
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Problems solved by technology

The use of enzymes greatly increases the cost of detection, and its instability is likely to cause large differences in analysis results; the inherent disadvantages of photobleaching in fluorescence technology limit the improvement of its detection sensitivity; although electrochemical analysis methods have the advantages of sensitivity and low cost , but the complex built-in electrode fabrication process on the digital microfluidic chip limits its development

Method used

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  • Target detecting method and system based on digital microfluidic technology and surface-enhancement Raman scattering technology
  • Target detecting method and system based on digital microfluidic technology and surface-enhancement Raman scattering technology
  • Target detecting method and system based on digital microfluidic technology and surface-enhancement Raman scattering technology

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Embodiment Construction

[0023] 1. Fabrication of chips

[0024]As shown in Figure 1, the digital microfluidic chip is divided into two parts, the upper plate and the lower plate. The processing technology of the lower plate is complex, including the substrate, electrode array, dielectric layer and hydrophobic layer; the upper plate includes the base, the conductive layer and insulating hydrophobic layer. The electrode array of the lower plate consists of 30 drive electrodes (each measuring 2.2mm×2.2mm), 6 reservoir electrodes (each measuring 5.4mm×3.6mm), with a gap of 20 μm between the electrodes, and A single row of 48 contact electrodes is used to connect the drive system.

[0025] The specific production process of the lower plate:

[0026] (1) The lower plate uses quartz glass as the substrate, evaporates a 300nm thick chromium layer by magnetron sputtering, and then forms an electrode array with a specific pattern by photolithography and wet etching techniques;

[0027] (2) The dielectric la...

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Abstract

The invention belongs to the technical field of digital microfluidics, and particularly relates to a target detecting method based on a digital microfluidic technology and a surface-enhancement Ramanscattering technology. The system mainly comprises a digital microfluidic chip and a surface-enhancement Raman probe. The digital microfluidic chip consists of an upper polar plate and a lower polar plate. The surface-enhancement Raman probe comprises a metal core, a Raman report molecule and a shell layer, and the Raman report molecule is embedded between core-shell structures. By the method automated control on discrete droplets on an electrode array is implemented based on a dielectric wetting principle, a reaction system on a chip is constructed, and Raman signals are output rapidly in real time. The target detecting method has the advantages of full automatic processing, simplicity and rapidness, high sensitivity, suitability to a complicated biosystems and the like. A plurality of samples are simultaneously detected in parallel by program control; and the target detecting method can be widely applied to detection of various types of targets, and particularly can be used for detecting rare samples and infectious samples.

Description

technical field [0001] The invention belongs to the technical field of digital microfluidics, and mainly relates to a target detection method based on digital microfluidics technology and surface-enhanced Raman scattering technology. Background technique [0002] Digital Microfluidics (Digital Microfluidics, DMF) is an emerging microfluidic technology that realizes precise manipulation of individual discrete droplets on a microelectrode array based on the dielectric wetting effect. Droplet movement, generation, mixing, and splitting can be achieved by applying voltages to different electrodes on the electrode array (1, Miller, E.M.; Ng, A.H.; Uddayasankar, U.; Wheeler, A.R. Anal Bioanal Chem 2011, 399, 337- 345; 2. Choi, K.; Ng, A.H.; Fobel, R.; Wheeler, A.R. Annu Rev Anal Chem 2012, 5, 413-440.). Due to its high flexibility and strong reconfigurability, digital microfluidic chips are easy to combine with other technologies such as optics, electrochemistry, mass spectrometr...

Claims

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Application Information

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IPC IPC(8): G01N21/65B01L3/00
CPCB01L3/502707B01L3/50273B01L3/502784B01L2400/0415G01N21/658
Inventor 杨朝勇王杨阮庆宇郑萍萍林水潮朱志周雷激
Owner XIAMEN UNIV
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