Repair spray containing high-concentration human mesenchymal stem cell extracts for small pet and preparation method of repair spray

A technology of mesenchymal stem cells and extracts, which is applied in the field of repair sprays containing high-concentration human mesenchymal stem cell extracts and the preparation thereof, can solve the problems of discount of the function of living cells, harsh living conditions of living cells, easy death, etc. Endometrial tissue differentiation, reducing bacterial infection and inflammatory response, and promoting regeneration and repair

Inactive Publication Date: 2017-09-19
TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been reports on the application of mesenchymal stem cells in damage repair, but due to the harsh survival conditions of active cells, they are easy to die, so the role played by living cells will be greatly reduced

Method used

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  • Repair spray containing high-concentration human mesenchymal stem cell extracts for small pet and preparation method of repair spray
  • Repair spray containing high-concentration human mesenchymal stem cell extracts for small pet and preparation method of repair spray
  • Repair spray containing high-concentration human mesenchymal stem cell extracts for small pet and preparation method of repair spray

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preparation example Construction

[0021] The preparation method of the repair spray containing high-concentration human mesenchymal stem cell extract for small pets of the present invention comprises the following steps:

[0022] (1) Human mesenchymal stem cell culture: P2-P6 human mesenchymal stem cells were selected from the cell bank for resuscitation, the inoculation batches were recorded as the first batch, the second batch, and the third batch, and the inoculation density was the maximum fusion 50%-60%, 35%-45%, 20%-30% of the degree; the complete medium used for culturing cells is phenol red-free DMEM / DF12 and the volume ratio concentration is 10% fetal bovine serum; the cell inoculation is completed and placed in Cultivate in a carbon dioxide incubator at a temperature of 37°C and a concentration of 5% by volume of carbon dioxide;

[0023] (2) When the fusion degree of the first batch of cells reaches 70%-80%, wash the cells twice with normal saline, and perform starvation culture with compound electro...

Embodiment 1

[0035] (1) Human umbilical cord mesenchymal stem cell culture: P4 human umbilical cord mesenchymal stem cells were selected from the cell bank for recovery, the total number of cells was 8.4*10 7 . The inoculation batches were recorded as the first batch, the second batch, and the third batch, and the inoculation density was 60% of the maximum fusion degree (the number of inoculated cells per bottle: 9*10 6 , six bottles of cells inoculated), 40% (the number of cells inoculated in each bottle: 6*10 6 , four bottles of inoculated cells), 20% (number of inoculated cells: 3*10 6 , inoculate two bottles of cells), the culture system is 40ml. The complete medium used for culturing the cells is phenol red-free DMEM / DF12 and 10% fetal calf serum by volume, and the bottle for inoculating the cells is a T-175 culture flask. After the cell inoculation is completed, place it in a carbon dioxide incubator for cultivation at a temperature of 37°C and a volume ratio concentration of carb...

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Abstract

The invention discloses repair spray containing high-concentration human mesenchymal stem cell extracts for a small pet and a preparation method of the repair spray. The repair spray mainly comprises the high-concentration mesenchymal stem cell extracts. Mesenchymal stem cells can secrete various cell factors in the culture process, the cell factors can penetrate into an endomembrane base layer, endomembrane tissue differentiation, vessel regeneration and granulation tissue growth are promoted, injured skin tissue structure regeneration and repair are promoted, generation of scar connective tissues is decreased, a skin immune microenvironment is regulated, and bacterial infection and inflammatory reaction of wound tissues are decreased. By a continuous mesenchymal stem cell starving method, high-concentration human umbilical cord mesenchymal extracts can be obtained in a short time. The repair spray containing the high-concentration mesenchymal stem cell extracts is applicable to repair of eczema, chronic surface ulcers and various skin injuries.

Description

technical field [0001] The invention relates to stem cell extracts, in particular to a repairing spray containing high-concentration human mesenchymal stem cell extracts for small pets and a preparation method thereof. Background technique [0002] Mesenchymal stem cells (MSCs) are a group of heterogeneous cell populations derived from stroma, which can be obtained from most tissues of the human body. They have the ability of self-renewal, tissue repair, and immune regulation, and can differentiate to the mesoderm lineage, such as adipocytes, bone cells, chondrocytes, etc., and can also differentiate to other germ layer lineage cells, such as epidermal cells, vascular endothelium Cell Differentiation. [0003] The mesenchymal stem cell culture system contains a variety of active ingredients, such as: stem cell growth factor (SCF), fibroblast growth factor (FGF), endothelial cell growth factor (VEGF), epidermal growth factor (EGF), cell Colony-stimulating factor, interleuki...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K9/12C12N5/0775A61P17/00A61P17/02A61P31/04
CPCA61K35/28A61K9/0014A61K9/12C12N5/0664C12N5/0667C12N5/0668
Inventor 熊亮张冰晶鲁振宇韩洪起秦臻高婧彧徐悦黄文敬
Owner TIANJIN PURUI SAIER BIOLOGICAL TECH CO LTD
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