Method for inducing adventitious buds of hybrid eucalyptus isolated organs through callus tissue differentiation

Abstract

The invention relates to a method for inducing adventitious buds of hybrid eucalyptus isolated organs through callus tissue differentiation. The method comprises: an axillary bud acquisition step: clipping a bud strip from hybrid eucalyptus, disinfecting the bud strip, and inoculating the disinfected bud strip into a culture medium so as to carry out germination culture to grow axillary buds; a rooting induction culture step: cutting the axillary buds, transferring the axillary buds to an MS (Murashige and Skoog) culture medium so as to carry out successive transfer culture to grow sprouts which meet requirements, cutting the sprouts and transferring the sprouts to a 1 / 2 MS culture medium so as to carry out rooting induction culture to obtain a rooted seedling; a sprout culture step: cutting off the terminal buds of the rooted seedling, reserving 1-4 axillary buds of root and base parts, inoculating the axillary buds onto the MS culture medium so as to carry out sprout culture; a callus tissue induction culture step: inoculating a cut sprout section utilized as an explant onto a callus induction culture medium so as to culture under a dark condition and then under a weak illumination condition, thus obtaining callus tissues; and an adventitious bud differentiation step: transferring the callus tissues onto a differential medium so as to carry out successive transfer culture, thus obtaining the adventitious buds. The method is based on the bud strip of clonal adult eucalyptus, thus the regeneration efficiency reaches about 60%.

Description

technical field

[0001] The invention relates to the technical field of biological tissue culture and seedling cultivation, in particular to a method for inducing hybrid eucalyptus isolated organs to differentiate into adventitious buds through callus. Background technique

[0002] Eucalyptus is a species of Eucalyptus in the family Myrtaceae (Myrtaceae) Eucalyptus) Generic name for plants. It is one of the three fast-growing tree species in the world. Widely distributed in Australia, South America, Asia, Europe and other regions. Eucalyptus is widely used in cellulose industry, pulpwood and fiberboard, and can also be used as a raw material for extracting tannin and essential oil. Eucalyptus extract is used in many fields such as chemical industry and medical treatment, and has been widely used in many countries and regions. to plant. Therefore, eucalyptus has extensive development and utilization value.

[0003] Hybrid eucalyptus plays an important role in the field of...