The invention provides a cherry biological antibacterial antistaling agent, which is characterized in that it is composed of the following raw materials in mass ratio: eucalyptus leaf extract 1.0-5.0%, cassia bark extract 0.5-2.5%, patchouli extract 5.0-15.0%, chlorinated Calcium 0.5-5.0%, phytic acid 0.1-2.0%, and the balance is water. The use of this preservative not only effectively inhibits the growth of mold and prevents the rot of cherry fruit, but also reduces the evaporation of water in the fruit, prevents the oxidation between the oxygen in the air and the components of the fruit, reduces the respiration intensity, and increases the surface of the fruit. Brightness improves its appearance and greatly increases the value of the product. When stored at 0-4°C for 20 days, the good fruit rate reaches 92%, and the water loss rate is ≤1.7%. Even if it is stored at room temperature, it can prevent moisture Lost, shrunken and wilted, stored at room temperature for 10 days, the good fruit rate is 85%, and the water loss rate is ≤3.0%, so as to better maintain the nutritional value, color, aroma, taste and shape of cherry fruit, and prolong the shelf life of the fruit , will not cause damage to human health.