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Virus diluent for inoculating chick embryo and preparation method of virus diluent

The technology of diluent and virus is applied in the field of virus diluent for inoculating chicken embryos and its preparation field, which can solve the problems of reduced poison price, low content of allantoic fluid, etc. The effect of prolonging the proliferation time

Active Publication Date: 2015-04-22
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, 10-day-old chicken embryos are used for Newcastle disease virus inoculation and breeding. Generally, chicken embryo fluid is harvested after 96-120 hours of cultivation, that is, 14-15 days old. At this time, the age is too old and the allantoic fluid content is not high (chicken embryo The allantoic fluid content is the highest at the age of 13 to 14 days of development); if the allantoic fluid content is increased, it must be harvested in advance, but the virus content has not reached the peak at this time, and the reduction of the poison price is not worth the loss.

Method used

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  • Virus diluent for inoculating chick embryo and preparation method of virus diluent

Examples

Experimental program
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Effect test

Embodiment 1

[0044]A virus dilution for inoculating chicken embryos, the virus dilution contains in every 1000ml phosphate buffer: glucose 25g, amino acid combination 3.3g (wherein L-proline 0.8g, glycine 0.2g, tyrosine 0.5g, cysteine amino acid 1.2g, serine 0.6g), vitamin combination 7g (including vitamin B1 2g, vitamin C 4g, vitamin E 1g), compound Chinese medicine polysaccharide 50g, sodium selenite 3mg, the pH of the diluent is 6.8.

[0045] A preparation method for virus dilution for inoculating chicken embryos, the steps are:

[0046] (1) Preparation of phosphate buffer solution: Accurately weigh 8 g of sodium chloride, 0.2 g of potassium chloride, 1.15 g of disodium hydrogen phosphate, 0.2 g of potassium dihydrogen phosphate, and 0.1 g of calcium chloride according to the above formula of phosphate buffer solution. Dissolve 0.1 g of magnesium chloride containing 6 crystal waters in 1000 ml of double distilled water, pass through a 0.22 μm filter membrane under aseptic conditions, an...

Embodiment 2

[0056] A virus dilution for inoculating chicken embryos, the virus dilution contains in every 1000ml phosphate buffer: glucose 40g, amino acid combination 4g (wherein L-proline 1g, glycine 0.4g, tyrosine 0.5g, cystine 1.4g, serine 0.7g), vitamin combination 8g (including vitamin B1 2g, vitamin C 4.5g, vitamin E 1.5g), compound Chinese medicine polysaccharide 60g, sodium selenite 8mg, the pH of the diluent is 7.0.

[0057] A preparation method for virus dilution for inoculating chicken embryos, the steps of which are the same as in Example 1.

Embodiment 3

[0059] A virus dilution for inoculating chicken embryos, the virus dilution contains in every 1000ml phosphate buffer: glucose 50g, amino acid combination 5g (wherein L-proline 1.5g, glycine 0.75g, tyrosine 0.5g, cystine acid 1g, serine 1.25g), vitamin combination 10g (including vitamin B1 3.5g, vitamin C 5.5g, vitamin E 1g), compound Chinese medicine polysaccharide 100g, sodium selenite 10mg, the pH of the diluent is 6.6. The above-mentioned phosphate buffer contains 6 g of sodium chloride, 0.05 g of potassium chloride, 1 g of disodium hydrogen phosphate, 0.05 g of potassium dihydrogen phosphate, 0.05 g of calcium chloride, and 0.05 g of magnesium chloride containing 6 crystal waters.

[0060] A preparation method for virus dilution for inoculating chicken embryos, the steps of which are the same as in Example 1.

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Abstract

The invention discloses a virus diluent for inoculating a chick embryo and a preparation method of the virus diluent, belonging to the technical field of animal biological products. The virus diluent for inoculating the chick embryo contains the following components in weight ratio: 10-50g of glucose, 3-5g of amino acid composition, 6-10g of vitamin composition, 0-10mg of sodium selenite and 30-100g of compound traditional Chinese medicine polysaccharide in every 1000ml of phosphate buffer solution. After a virus diluted by using the virus diluent is inoculated to the chick embryo, the virus titer and the quantity of the obtained virus liquid can be simultaneously increased.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a virus diluent for inoculating chicken embryos and a preparation method thereof. Background technique [0002] At present, there are three methods for virus culture: animal inoculation, tissue culture and chicken embryo culture. Among them, chicken embryo culture is one of the most commonly used methods in virus culture. Chicken embryos are developing organisms, and a variety of animal viruses can multiply and pass on in chicken embryos. The advantage of chicken embryos is that the tissue differentiation degree of embryos is low, and different ages and inoculation routes can be selected. Viruses are easy to multiply in chicken embryos. Some viruses can produce watercress after infecting chicken embryos, causing congestion, hemorrhage, necrosis and death. and other specific signs of infection. Virus-infected chick embryo tissues and liquids c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N7/00C12R1/93
Inventor 李阳闫艳丽刘雪王二先徐晓艳盛璐丝
Owner 浙江美保龙生物技术有限公司
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