Newcastle disease and bird flu specificity virus diluent and preparation method thereof
A technology for chicken Newcastle disease and avian influenza, applied in biochemical equipment and methods, virus/bacteriophage, and microorganism-based methods, etc., can solve problems such as large differences between batches, affect vaccine quality, and trouble production of varieties, so as to reduce batches The effect of improving the ability to tolerate viruses and improving stability
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Embodiment 1
[0036] A chicken Newcastle disease, bird flu specific virus dilution, the virus dilution contains per 1000ml of phosphate buffer: amino acid combination 3g (including aspartic acid 0.9g, proline 0.9g, glutamine 1.2g), vitamin Combination 5g (including vitamin B11.5g, vitamin B31.75g, vitamin B71.75g), compound traditional Chinese medicine polysaccharide 40g, sodium selenite 4mg, the pH of the diluent is 7.0.
[0037] A kind of preparation method of chicken Newcastle disease, avian influenza specific virus dilution, its step is:
[0038] (1) Preparation of phosphate buffer solution: Accurately weigh 8 g of sodium chloride, 0.2 g of potassium chloride, 1.15 g of disodium hydrogen phosphate, 0.2 g of potassium dihydrogen phosphate, and 0.1 g of calcium chloride according to the above formula of phosphate buffer solution. Dissolve 0.1 g of magnesium chloride containing 6 crystal waters in 1000 ml of double distilled water, pass through a 0.22 μm filter membrane under aseptic condi...
Embodiment 2
[0048]A chicken Newcastle disease, avian influenza specific virus dilution, this virus dilution is every 1000ml phosphate buffer (made of sodium chloride 10g, potassium chloride 0.5g, disodium hydrogen phosphate 1.2g, potassium dihydrogen phosphate 0.5g, chloride Calcium 0.2g, magnesium chloride 0.2g containing 6 crystal waters dissolved in 1000ml double distilled water) contains: amino acid combination 4g (including aspartic acid 2g, proline 0.8g, glutamine 1.2g), vitamin combination 6g (including vitamin B12.1g, vitamin B31.2g, vitamin B72.7g), compound Chinese medicine polysaccharide 50g (extracted from 20 parts of seaweed, 5 parts of Kochia scoparia, 10 parts of mulberry leaves, 5 parts of morning glory and 10 parts of salvia miltiorrhiza ), sodium selenite 2mg, the pH of the diluent is 7.0.
[0049] A kind of preparation method of chicken Newcastle disease, avian influenza specific virus diluent, its steps are the same as embodiment 1.
Embodiment 3
[0051] A chicken Newcastle disease, avian influenza specific virus dilution, the virus dilution per 1000ml phosphate buffer (made of sodium chloride 6g, potassium chloride 0.05g, disodium hydrogen phosphate 1g, potassium dihydrogen phosphate 0.05g, calcium chloride 0.05g, 0.05g of magnesium chloride containing 6 crystal water dissolved in 1000ml of double distilled water) contains: amino acid combination 2g (including aspartic acid 0.4g, proline 0.8g, glutamine 0.8g), vitamin combination 3g (of which vitamin B10.45g, vitamin B31.5g, vitamin B71.05g), compound Chinese medicine polysaccharide 20g (40 parts of seaweed, 20 parts of Kochia scoparia, 30 parts of mulberry leaves, 20 parts of morning glory and 30 parts of salvia miltiorrhiza), sub Sodium selenate 10mg, the pH of the diluent is 7.2.
[0052] A kind of preparation method of chicken Newcastle disease, avian influenza specific virus diluent, its steps are the same as embodiment 1.
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