Newcastle disease and bird flu specificity virus diluent and preparation method thereof

A technology for chicken Newcastle disease and avian influenza, applied in biochemical equipment and methods, virus/bacteriophage, and microorganism-based methods, etc., can solve problems such as large differences between batches, affect vaccine quality, and trouble production of varieties, so as to reduce batches The effect of improving the ability to tolerate viruses and improving stability

Inactive Publication Date: 2016-03-02
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far there are still defects in the technology of homeomorphic culture, especially the homeomorphic culture of chicken Newcastle disease and avian influenza. During the culture process, due to mutual interference between viruses, the content of the two viruses is not stable, and the difference between batches is relatively large. Large, troubled the production of this variety, seriously affected the quality of later vaccines

Method used

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  • Newcastle disease and bird flu specificity virus diluent and preparation method thereof
  • Newcastle disease and bird flu specificity virus diluent and preparation method thereof
  • Newcastle disease and bird flu specificity virus diluent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] A chicken Newcastle disease, bird flu specific virus dilution, the virus dilution contains per 1000ml of phosphate buffer: amino acid combination 3g (including aspartic acid 0.9g, proline 0.9g, glutamine 1.2g), vitamin Combination 5g (including vitamin B11.5g, vitamin B31.75g, vitamin B71.75g), compound traditional Chinese medicine polysaccharide 40g, sodium selenite 4mg, the pH of the diluent is 7.0.

[0037] A kind of preparation method of chicken Newcastle disease, avian influenza specific virus dilution, its step is:

[0038] (1) Preparation of phosphate buffer solution: Accurately weigh 8 g of sodium chloride, 0.2 g of potassium chloride, 1.15 g of disodium hydrogen phosphate, 0.2 g of potassium dihydrogen phosphate, and 0.1 g of calcium chloride according to the above formula of phosphate buffer solution. Dissolve 0.1 g of magnesium chloride containing 6 crystal waters in 1000 ml of double distilled water, pass through a 0.22 μm filter membrane under aseptic condi...

Embodiment 2

[0048]A chicken Newcastle disease, avian influenza specific virus dilution, this virus dilution is every 1000ml phosphate buffer (made of sodium chloride 10g, potassium chloride 0.5g, disodium hydrogen phosphate 1.2g, potassium dihydrogen phosphate 0.5g, chloride Calcium 0.2g, magnesium chloride 0.2g containing 6 crystal waters dissolved in 1000ml double distilled water) contains: amino acid combination 4g (including aspartic acid 2g, proline 0.8g, glutamine 1.2g), vitamin combination 6g (including vitamin B12.1g, vitamin B31.2g, vitamin B72.7g), compound Chinese medicine polysaccharide 50g (extracted from 20 parts of seaweed, 5 parts of Kochia scoparia, 10 parts of mulberry leaves, 5 parts of morning glory and 10 parts of salvia miltiorrhiza ), sodium selenite 2mg, the pH of the diluent is 7.0.

[0049] A kind of preparation method of chicken Newcastle disease, avian influenza specific virus diluent, its steps are the same as embodiment 1.

Embodiment 3

[0051] A chicken Newcastle disease, avian influenza specific virus dilution, the virus dilution per 1000ml phosphate buffer (made of sodium chloride 6g, potassium chloride 0.05g, disodium hydrogen phosphate 1g, potassium dihydrogen phosphate 0.05g, calcium chloride 0.05g, 0.05g of magnesium chloride containing 6 crystal water dissolved in 1000ml of double distilled water) contains: amino acid combination 2g (including aspartic acid 0.4g, proline 0.8g, glutamine 0.8g), vitamin combination 3g (of which vitamin B10.45g, vitamin B31.5g, vitamin B71.05g), compound Chinese medicine polysaccharide 20g (40 parts of seaweed, 20 parts of Kochia scoparia, 30 parts of mulberry leaves, 20 parts of morning glory and 30 parts of salvia miltiorrhiza), sub Sodium selenate 10mg, the pH of the diluent is 7.2.

[0052] A kind of preparation method of chicken Newcastle disease, avian influenza specific virus diluent, its steps are the same as embodiment 1.

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Abstract

The invention relates to a newcastle disease and bird flu specificity virus diluent and a preparation method thereof and belongs to the technical field of animal biological products. Every 1000 ml of phosphate buffer in the diluent comprises, by weight, 2-4 g of amino acid combination, 3-6 g of vitamin combination, 0-10 mg of sodium selenite and 20-50 g of compound traditional Chinese medicine polysaccharide. The amino acid combination comprises aspartic acid, proline and glutamine. The vitamin combination comprises vitamin B1, vitamin B3 and vitamin B7. The compound traditional Chinese medicine polysaccharide is obtained by extraction from algae, fructus kochiae, mulberry leaf, semen pharbitidis and salviae miltiorrhizae. The virus diluent obtained through culture according to the method improves homeomorphic cultivation stability and greatly reduces batch difference, and excellent antigen liquid is provided for a follow-up vaccine production process.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a chicken Newcastle disease and avian influenza specific virus dilution and a preparation method thereof. Background technique [0002] Newcastle disease and avian influenza are the most prevalent poultry infectious diseases in the world today, and they are also the two most harmful diseases among all chicken diseases in my country. The preparation of the double vaccine can prevent and treat two kinds of infectious diseases with one injection, and at the same time, it can solve other adverse reactions of chicken flocks caused by the use of multiple vaccines in divided immunizations, simplify the immunization procedure, and reduce the input cost. At present, the dual-inactivated vaccines for Newcastle disease and avian influenza produced in China are basically prepared by inoculating chicken embryos with viruses, multiplying them, and harvesting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00C12R1/93
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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