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Method for testing low-temperature-resistant character of Litopenaeus vannamei

A test method and low temperature resistance technology, applied in the field of genetics and breeding, can solve the problems of multi-energy, consumption, influence on shrimp breeding output and economic benefit, etc., and achieve the effect of improving low temperature resistance ability and improving breeding economic effect.

Pending Publication Date: 2018-11-09
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] The suitable temperature range limits the breeding season and region of prawns, and it cannot cope with frequent extreme cold weather. In addition, due to the relatively low temperature in winter and in the north, more energy will be consumed in the cultivation of Litopenaeus vannamei, which directly affects the quality of prawns. Breeding Yield and Economic Benefits

Method used

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  • Method for testing low-temperature-resistant character of Litopenaeus vannamei
  • Method for testing low-temperature-resistant character of Litopenaeus vannamei
  • Method for testing low-temperature-resistant character of Litopenaeus vannamei

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Embodiment

[0022] The methods for testing the low-temperature resistance traits of prawns include: 1) slow cooling and temporary cultivation; 2) low-temperature stress treatment; 3) data and sample collection. Specific steps are as follows:

[0023] 1) Slowly cool down and keep for a while

[0024] Select prawn families with neat specifications and healthy physique as the test object, put them into a sorting box of appropriate size, put a thermometer in each sorting box, put the sorting box in a refrigerator frame of 72cm×37cm×20cm, and finally place the refrigerator frame Put it into a 183cm×84cm×92cm refrigerator with a temperature sensor and an adjustable temperature to inflate and raise it temporarily. Before the start of the experiment, the shrimp was slowly lowered from normal temperature (24°C-28°C) to 18°C ​​at a rate of 1°C / d per day, and then the low temperature stress treatment was started.

[0025] 2) Low temperature stress treatment

[0026] The low-temperature tolerance ...

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Abstract

The invention provides a method for testing the low-temperature-resistant character of Litopenaeus vannamei. The method comprises the following steps: 1) slow cooling for temporary rearing, 2) low-temperature stress treatment and 3) data and sample collection, wherein cooling rate in step 1) is 1 DEG C / d; cooling rate in step 2) is 2 DEG C / d; families and individuals with good low-temperature-resistant character are determined according to low-temperature accumulated survival time and temperature at death time. One novel method is provided for improving low-temperature adaptability of prawns,with adoption of the method, low-temperature tolerance of the Litopenaeus vannamei can be improved remarkably, energy cost for maintaining high temperature in a breeding process is reduced, and breeding economic benefit is increased.

Description

technical field [0001] The invention relates to the technical field of genetic breeding, in particular to a method for testing the low temperature resistance of Litopenaeus vannamei. Background technique [0002] Litopenaeus vannamei, also known as Penaeus vannamei, also known as white-legged shrimp, belongs to the phylum Arthropoda, the class Crustacea, the order Decapoda, the family Penaeidae, and the genus Litopenaeus ( Litopenaeus). Litopenaeus vannamei is native to the coastal waters of Ecuador, a Central and South American country. It is a tropical bottom-dwelling species. The optimum growth water temperature range is 22-35°C, and it almost stops feeding when it is lower than 18°C. Vannamei is the aquaculture species with the highest single production value in the world. Since it was introduced into my country in 1988, because of its high meat yield, high stress resistance, strong adaptability, fast growth, high-density and low-salinity farming, suitable for intensiv...

Claims

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Application Information

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IPC IPC(8): A01K61/59
CPCA01K61/59Y02A40/81
Inventor 孟宪红孔杰曹宝祥陈宝龙罗坤栾生曹家旺
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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