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Method of using silver nanocluster to detect potassium ion concentration in vitreous fluid to deduce death time

A technique of potassium ion concentration and time of death

Inactive Publication Date: 2017-02-15
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these electrodes will age with time and are more expensive

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  • Method of using silver nanocluster to detect potassium ion concentration in vitreous fluid to deduce death time
  • Method of using silver nanocluster to detect potassium ion concentration in vitreous fluid to deduce death time
  • Method of using silver nanocluster to detect potassium ion concentration in vitreous fluid to deduce death time

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Embodiment 1

[0041] The method of detecting the concentration of potassium ions in the vitreous humor by silver nanoclusters in this embodiment and estimating the time of death is carried out according to the following steps:

[0042] (1) Preparation of fluorescent DNA-AgNCs: Take 90 μL of 50 μM DNA solution at 4°C at 12000 rpm, take 50 μL of the bottom layer liquid into a centrifuge tube, and add 54 μL of 500 μM AgNO in the centrifuge tube 3 solution and 102 μL of secondary water, shake at room temperature for 30 minutes; subsequently, add 54 μL of 500 μM NaHB prepared in ice water 4 solution, shaken for 5 minutes, and stored in a 4°C refrigerator for 3 hours to obtain a brownish-yellow liquid, that is, a fluorescent DNA-AgNCs solution. ;

[0043] The DNA chain nucleotide sequence used in this example is the DNA sequence shown in the nucleotide sequence table ID NO.6;

[0044] (2) Preparation of potassium ion concentration and fluorescence intensity standard curve: preparation concentra...

Embodiment 2

[0049] The method of detecting the concentration of potassium ions in the vitreous humor by silver nanoclusters in this embodiment and estimating the time of death is carried out according to the following steps:

[0050] (1) Preparation of fluorescent DNA-AgNCs: Take 100 μL of 50 μM DNA solution at 4°C at 12000 rpm, take 60 μL of the bottom layer liquid into a centrifuge tube, add 108 μL of 500 μM AgNO in the centrifuge tube 3 solution and 204 μL of secondary water, shake at room temperature for 30 minutes; subsequently, add 108 μL of 500 μM NaHB prepared in ice water 4 The solution was shaken for 5 minutes and stored in a refrigerator at 4°C for 3 hours to obtain a brownish yellow liquid, namely the fluorescent DNA-AgNCs solution. spare;

[0051] The DNA chain nucleotide sequence used in this example is the DNA sequence shown in the nucleotide sequence table ID NO.6;

[0052] (2) Prepare the standard curve of potassium ion concentration and fluorescence intensity: prepare ...

Embodiment 3

[0057] The method of detecting the concentration of potassium ions in the vitreous humor by silver nanoclusters in this embodiment and estimating the time of death is carried out according to the following steps:

[0058] (1) Preparation of fluorescent DNA-AgNCs: Take 100 μL of 50 μM DNA solution at 4°C at 12000 rpm, take 60 μL of the bottom layer liquid into a centrifuge tube, add 108 μL of 500 μM AgNO in the centrifuge tube 3 solution and 204 μL of secondary water, shake at room temperature for 30 minutes; subsequently, add 108 μL of 500 μM NaHB prepared in ice water 4 The solution was shaken for 5 minutes and stored in a refrigerator at 4°C for 3 hours to obtain a brownish yellow liquid, namely the fluorescent DNA-AgNCs solution. spare;

[0059] The DNA chain nucleotide sequence used in this example is the DNA sequence shown in the nucleotide sequence table ID NO.6;

[0060] (2) Prepare the standard curve of potassium ion concentration and fluorescence intensity: prepare ...

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Abstract

The invention belongs to the technical field of medicine, and particularly relates to a method of using silver nanocluster to detect potassium ion concentration in vitreous fluid to deduce death time. The method includes that a novel DNA strand containing potassium ion aptamer is used as a template to synthesize DNA-AgNCs having fluorescent property, the system specifically recognizes K+ to quench fluorescence of the silver nanocluster, and a standard curve is drawn according to changing of the fluorescence; vitreous fluid to be detected is added into a DNA-AgNCs solution, potassium ions in the vitreous fluid lead to changing of the fluorescence of the silver nanocluster, the potassium ion concentration in the detected vitreous fluid is acquired by comparing with the standard curve; a standard curve between K+ concentration in a vitreous fluid sample and known death time is drawn, and a result has high uniformity to reported death time and vitreous fluid potassium ion relation. The method can be used for deducing death time by legal medical experts, has high specificity and sensitivity, is simple and quick to operate and low in cost and can be applied to detecting potassium ion content in other body fluids and water environment.

Description

technical field [0001] The invention belongs to the technical field of forensic medicine, and in particular relates to a method for detecting the concentration of potassium ions in vitreous humor by silver nanoclusters and then estimating the time of death. Background technique [0002] Potassium ion (K + ) is one of the most abundant cations in the human body and has important physiological functions for organisms. In order to maintain the normal acid-base balance and osmotic pressure of the body, it is necessary to participate in the metabolism of various proteins and sugars; it is one of the important electrolytes to maintain cell metabolism, regulate body fluid osmotic pressure, maintain acid-base balance and maintain cell stress function. Its content is a very important indicator of the body's physiological activities. At the same time, the content of potassium ions in blood and urine can be used to diagnose diseases such as the heart and kidneys and judge the prognosi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 丁艳君凌江李杏梅蔡继峰郭亚东扎拉嘎白乙拉
Owner CENT SOUTH UNIV
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