Beauveria bassiana strain having high pathogenicity to silkworms and application thereof

A highly pathogenic technology of Beauveria bassiana, applied in the field of silkworm production, can solve the problems of low success rate of silkworm ossification and low yield of silkworms, and achieve neat death time of silkworms, high ossification rate and strong pathogenicity Effect

Active Publication Date: 2014-07-16
SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

[0005] In order to overcome the low ossified success rate of silkworms caused by the lack of excellent Beauveria bassiana strains and the low yield of silkworms, the primary purpose of the present invention is to provide a Beauveria bassiana strain with high pathogenicity to silkworms

Method used

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  • Beauveria bassiana strain having high pathogenicity to silkworms and application thereof
  • Beauveria bassiana strain having high pathogenicity to silkworms and application thereof
  • Beauveria bassiana strain having high pathogenicity to silkworms and application thereof

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Embodiment

[0027] 1. Isolation and identification of Beauveria bassiana bacterial strains of the present invention

[0028] (1) Separation and purification

[0029] Bombyx mori of the present invention is collected from Guangdong Luoding silkworm area, and the silkworm kind is " spring 5 ".

[0030] After the collected silkworms are fully ossified, the white powder on the body surface is gently shaken off into a conical flask filled with a 0.2% Tween-80 aqueous solution, and the conidia of Beauveria bassiana are separated by vigorous shaking with a magnetic stirrer. . Dilute the spore suspension in a gradient, dip it with a sterilized triangular glass smear stick, spread it evenly on a PDA medium plate, and culture it at 26°C and 90% humidity.

[0031] When a single pure white colony grows, use an inoculation needle to pick up the colony and transfer it to another sterilized PDA medium plate in time, continue to cultivate and observe that there are no other bacteria, and the purificati...

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Abstract

The invention discloses a Beauveria bassiana strain having high pathogenicity to silkworms and an application of the Beauveria bassiana strain in the production of Bombyx Batryticatus. The strain is preserved at the China General Microbiological Culture Collection Center on February 24, 2014 and the preservation number is CGMCC NO. 8861. The method for producing Bombyx Batryticatus by using the strain disclosed by the invention comprises the steps of preparing a spore suspension by using Beauveria bassiana and sterile water containing 0.2% of Tween-80, uniformly spraying the spore suspension onto the fifth-instar silkworm and inoculating for 4-5 days and obtaining Bombyx Batryticatus after a large quantity of silkworm died from muscardine. The Beauveria bassiana strain disclosed by the invention has high pathogenicity to the silkworm, the death time of the silkworms is consistent, the rigescence rate is high and is conducive to solving the problems about the standard production, quality control of Bombyx Batryticatus and the like.

Description

technical field [0001] The invention belongs to the field of silkworm production, and in particular relates to a Beauveria bassiana strain with high pathogenicity to silkworms and its application in silkworm production. Background technique [0002] Bombyx mori is the dead body of Bombyx mori L.4-5 instar larvae infected (or artificially inoculated) Beauveria bassiana (Bals.) Vuillant. It is an excellent Chinese medicinal material and is used in Chinese patent medicines It is widely used in traditional Chinese medicine and prescriptions, and the annual demand is about 1,000 tons. According to statistics, 49 out of 513 kinds of human patent medicines contain white silkworm, and 29 kinds of 64 kinds of pediatric patent medicines contain white silkworm, such as Qizhen pills, Taiji Shengsheng pills, Xiaoer Qiying pills, Xiaoer Zhenjing pills, etc. . [0003] However, at present, silkworms mainly rely on loose collection from farmers in sericulture areas. No matter in terms of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01K67/04C12R1/645
Inventor 邢东旭杨琼李丽廖森泰罗国庆肖阳李庆荣叶明强
Owner SERICULTURE & AGRI FOOD RES INST GUANGDONG ACAD OF AGRI SCI
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