Production method of white muscardine silkworms

A production method and technology of white silkworm, which is applied in animal husbandry and other directions, can solve the problems of long time of silkworm body rigidity, unstable quality and high cost, and achieve the effects of neat death time of silkworm body, short rigidity time and high rigidity rate.

Inactive Publication Date: 2017-05-31
SICHUAN ACAD OF AGRI SCI SERICULTURE INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing artificially produced white silkworm, the product quality can reach the index of authentic medicinal materials, and the production process is easier to control manually, but there are still some problems in the existing artificially produced white silkworm, such as the silkworm body after infection from death to The ossification time is longer, the ossification rate of the white silkworm is lower, the ossification time is irregular, the quality is unstable, the output is low, and the cost is also high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A kind of production method of white silkworm described in the present embodiment, comprises the following steps:

[0022] (1) Pathogen rejuvenation: adopt Beauveria bassiana to infect the silkworm body, collect fresh Beauveria bassiana spores from the silkworm body;

[0023] (2) pathogen attack: adjust the temperature of silkworm rearing to 24 DEG C, and the humidity of silkworm rearing is 82%, and the Beauveria bassiana spores collected in step (1) are formulated into Beauveria bassiana spore liquid, and the concentration of Beauveria bassiana spore liquid is 10 6 spores / mL, close the doors and windows, spray the Beauveria bassiana spore liquid evenly on the silkworm bodies of the 5th instar silkworm with a sprayer, the number of spraying times is not less than 2 times, and the closed space is 1h;

[0024] (3) silkworm body culture: open doors and windows for ventilation, the silkworm seat for cultivating silkworm body is sterilized with ofloxacin solution, the contro...

Embodiment 2

[0029] A kind of production method of white silkworm described in the present embodiment, comprises the following steps:

[0030] (1) Pathogen rejuvenation: adopt Beauveria bassiana to infect the silkworm body, collect fresh Beauveria bassiana spores from the silkworm body;

[0031] (2) Pathogen attack: adjust the temperature of silkworm rearing to 26 DEG C, and the humidity of silkworm rearing is 84%, and the Beauveria bassiana spores collected in step (1) are formulated into Beauveria bassiana spore liquid, and the concentration of Beauveria bassiana spore liquid is 10 7 spores / mL, close the doors and windows, spray the Beauveria bassiana spore liquid evenly on the silkworm bodies of the 5th instar silkworm with a sprayer, the number of spraying times is not less than 2 times, and the closed space is 1.5h;

[0032] (3) silkworm body culture: open the door and window for ventilation, the silkworm seat for cultivating silkworm body is sterilized with ofloxacin solution, the co...

Embodiment 3

[0037] A kind of production method of white silkworm described in the present embodiment, comprises the following steps:

[0038] (1) Pathogen rejuvenation: adopt Beauveria bassiana to infect the silkworm body, collect fresh Beauveria bassiana spores from the silkworm body;

[0039] (2) pathogen attack: adjust the temperature of silkworm rearing to 24 DEG C, and the humidity of silkworm rearing is 86%, and the Beauveria bassiana spores collected in step (1) are formulated into Beauveria bassiana spore liquid, and the concentration of the Beauveria bassiana spore liquid is 10 8 spores / mL, close the doors and windows, spray the Beauveria bassiana spore liquid evenly on the silkworm bodies of the 5th instar silkworm with a sprayer, the number of spraying times is not less than 2 times, and the closed space is 1.5h;

[0040] (3) silkworm body culture: open doors and windows for ventilation, the silkworm seat for cultivating silkworm body is sterilized with ofloxacin solution, the ...

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PUM

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Abstract

The invention discloses a production method of white muscardine silkworms. The production method comprises the following steps of: (1) rejuvenescence of pathogens: adopting white muscardine silkworms to infect bombyx mori polypides and collecting fresh beauveria bassiana on the bombyx mori polypides; (2) toxin attacking for the pathogens: preparing the beauveria bassiana into beauveria bassiana liquid, closing doors and windows, uniformly spraying the beauveria bassiana liquid on silkworm bodies with newly-exuviated fifth larva and placing in a sealed space for 1-2 hours; (3) culture of silkworm bodies: opening doors and windows for ventilation, controlling the temperature to 22-26 DEG C and the humidity to 95-98%, and culturing till the silkworm bodies are died; (4) culture of muscardine silkworms: choosing out died silkworm bodies, culturing on a clean silkworm seat paper, controlling the temperature to 24-28 DEG C and the humidity to more than or equal to 80%, culturing till rigidification of the died silkworm bodies, growing white hypha conidia and obtaining semi-finished products of the muscardine silkworms; and (5) preparation of finished products of the white muscardine silkworms: collecting semi-finished products of the white muscardine silkworms, airing and drying to obtain the finished products of the white muscardine silkworms. The production method disclosed by the invention has the advantages that the death time of the silkworm bodies is neat, the rigidification time is short, the rigidification rate is high, the yield of the white muscardine silkworms is high and the quality is stable.

Description

technical field [0001] The invention relates to a production method of white silkworm. Background technique [0002] The larvae of the insect silkworm are infected with Beauveria bassiana before they spin silk, and they become morbid and dead. The worm body becomes ossified, and the Beauveria bassiana hyphae and spores on the surface of the worm body and inside the worm body form the Beauveria bassiana after drying. White silkworm is a traditional Chinese medicine with high medicinal value. It tastes salty, pungent, and mild in nature. It can cure dark spots and facial disorders. White silkworm contains amino acids and active mercerin, which can nourish the skin and beautify the skin; The protein contained in it can stimulate the episebaceous glands and regulate the secretion of sex hormones, so it has a certain effect on the chloasma caused by the imbalance of female sex hormone secretion; it contains vitamin E, which can scavenge free radicals and resist senile plaques for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 青学刚李文学陈宝瑞刘刚张友洪周安莲肖文福黄盖群刘俊凤殷浩尹红王晓芬王皓
Owner SICHUAN ACAD OF AGRI SCI SERICULTURE INST
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