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Freezing method of goat epididymal sperms

A freezing method and sperm technology, applied in the field of biological reproduction, can solve problems such as low survival rate, inability to keep offspring normally, and inability to maximize the utilization of efficient genetic resources, achieving good results and beneficial effects for protection

Inactive Publication Date: 2019-05-21
柴局
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention solves the problems such as the inability to maximize the utilization of highly efficient genetic resources, the sudden death of excellent individuals, or the inability to keep offspring normally when alive, and the problem is more prominent in the application of wild animal protection; at the same time, the survival rate of sheep semen has been low after freezing and thawing. High, the present invention has studied a kind of animal testis that collects dead individual after animal's death, and hatches the method for spermatozoa from epididymis and freezes; And provides a kind of, can

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Testis collection samples come from local slaughterhouses (such as Yuquan District, Hohhot City, Inner Mongolia). Collect testes with scrotum from adult male white cashmere goats after slaughter. The collected testicles are placed in a box with gel ice packs. Note that the samples Do not directly contact with the ice pack to prevent the testes from cooling down too quickly, and then transport the samples to the Key Laboratory of Animal Genetics and Reproduction in Inner Mongolia Autonomous Region and transfer the samples to a 4°C refrigerator for 22 hours.

[0036] Sperm collection The testes were separated from the scrotum after being placed at 4°C for 22 hours (24 hours after death), and the sheath wrapped around the testis was separated from the testis, and the testis and epididymis were exposed. A thermometer probe was inserted into the incision to make it close to the cauda epididymis in order to monitor the temperature change of the epididymis at any time. The caud...

Embodiment 2

[0042] The epididymis was separated from the testis, and the operation process was divided into three treatment environments at room temperature (about 19°C), 4°C, and 37°C. The materials came from local slaughterhouses, different breeds of white cashmere goats and different individual weights. Testes were collected from the scrotum, placed in a pre-chilled ice box (a box with gel-like ice packs), and shipped to the Laboratory of Animal Genetics and Reproduction. The colloidal cooling ice box is conducive to transportation. After the samples are transported to the laboratory, the testes are placed in a refrigerator at 4°C for 22 hours, and then divided into three treatment groups in the experiment. 4 DEG C is to process two groups and 37 DEG C is to process three groups, other operations are the same as embodiment 1. Sperm were incubated from the epididymis at three different temperatures, and then the sperm indicators were measured with a microscope. The treatment group with ...

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Abstract

The invention discloses a freezing method of goat epididymal sperms. The method includes the following steps of firstly, separating testicles with scrotum from animal bodies to be stored; secondly, separating epididymis from the stored testicles with scrotum; thirdly, incubating sperms in the epididymis separated out; fourthly, processing the incubated sperms through a diluent and a mixed solution; fifthly, freeze-preserving the processed sperms at -250-0 DEG C, wherein the animal death time is 0-72 h and preferentially 0-24 h. Through the research on the freezing effect of the goat epididymalsperms, the method capable of successfully freeze-preserving the goat epididymal sperms is developed.

Description

technical field [0001] The invention relates to the field of biological reproduction, in particular to a method for freezing goat epididymis sperm. Background technique [0002] Sheep is the collective name of the Ovis subfamily, Mammalia, Artiodactyla, Bovidae, Ovis subfamily, and is one of human livestock. Hairy four-legged ruminant that is the main source of wool. The coat color is mainly white. my country mainly raises goats and sheep. [0003] Sheep are also known as sheep or white sheep. Hairy four-legged ruminant. It was originally a product of the mountains in the northern hemisphere, and is related to goats; the difference is that it is fatter, plump, and densely haired. short head. Males have large spiral horns, females have no or only small horns. [0004] Mammals, ruminants, generally have a pair of horns on the head, and there are many varieties, such as: sheep, Hu sheep, goats, blue sheep, etc. [0005] Goats (scientific name: Capra aegagrus hircus), al...

Claims

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Application Information

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IPC IPC(8): A01N1/00A01N1/02
Inventor 柴局张良斌张仙保布鲁木图李金泉邵东伟张新杰高振江康春义王惠梅
Owner 柴局
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