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Method for testing combined pollution toxicity of chlorpyrifos and butachlor

A technology of compound pollution and test method, which is applied in the test field of compound pollution toxicity of chlorpyrifos and butachlor, can solve problems such as compound pollution toxicity of chlorpyrifos and butachlor, and achieves avoiding pollution of relevant evaluation standards, increasing reference data, and facilitating objectivity. desired effect

Pending Publication Date: 2019-07-02
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a test method for the toxicity of chlorpyrifos and butachlor compound pollution in order to solve the above problems, which solves the shortcomings of existing equipment

Method used

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  • Method for testing combined pollution toxicity of chlorpyrifos and butachlor
  • Method for testing combined pollution toxicity of chlorpyrifos and butachlor
  • Method for testing combined pollution toxicity of chlorpyrifos and butachlor

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Embodiment

[0048] (1) Toxicity of chlorpyrifos and butachlor to zebrafish embryos:

[0049] After 96 hours of exposure, the mortality rate of zebrafish embryos in both the blank control group and the auxiliary control group was 50 The value is 170.1 (84.25-401.7) mg a.i.L -1 , the toxicity of chlorpyrifos increased with the prolongation of exposure time, and the toxicity increased significantly when exposed to 96h, and its LC 50 The value is 13.03 (7.54-19.71) mg a.i.L -1 , butachlor on 24h LC of zebrafish embryos 50 The value is 32.79 (23.26-63.39) mg a.i.L -1 , with the prolongation of exposure time, the toxicity increased significantly, and the LC at 48h, 72h and 96h 50 Values ​​were 5.82 (4.33-9.02), 4.42 (3.04-6.42) and 1.93 (1.37-3.55) mg a.i.L -1 , the toxicity of butachlor to zebrafish embryos at 96h is 6.75 times that of chlorpyrifos,

[0050] Table 1 Single toxicity of chlorpyrifos and butachlor to zebrafish embryos

[0051]

[0052] Exposure to chlorpyrifos and butach...

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Abstract

The invention discloses a method for testing the combine pollution toxicity of chlorpyrifos and butachlor. The method comprises the step that A, experimental organisms are prepared, specifically, a zebra fish wild type AB strain is adopted in an experiment and is domesticated in a laboratory after being purchased, and adult fish for collecting fish eggs for the experiment has been bred for more than one month in the laboratory. According to the method, a combined toxicity test and a single pesticide test are combined, so that reference data are conveniently increased according to the influenceof different environments and different reagents on animals, the cross reference of the data is improved, the influence of irrelevant variables is well balanced and counteracted, the experimental result is more persuasive, the toxicity of pesticides on fish fries is calculated by utilizing a probability value analysis method according to the mortality and the death time of the fish fries, reasonable analysis and treatment are carried out on the data, the phenomenon that related evaluation standard for pollution set by dependence on a single effect can be avoided, the objective requirements ofthe real environment quality can be conveniently and truly reflected, and therefore the environmental risk of pollutants in environmental water can be effectively evaluated, and the environmental threshold value which is safer to aquatic organisms can be formulated conveniently.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of toxicity test, in particular to a test method for the toxicity of compound pollution of chlorpyrifos and butachlor. [0003] Background technique: [0004] With the rapid development of modern industry and agriculture, more and more organic pollutants enter the environment through various channels and coexist in the environment. For a long time, toxicology research has mostly focused on the toxic effects of a single chemical substance. Many standards, such as waste water allowable discharge standards and safe concentration standards are also established based on the toxic effects of individual chemical substances. At present, many environmental toxicological effects cannot be explained by the mechanism of action of a single pollutant. Many studies have shown that Even if multiple pollutants are at the "safe" level of relevant water quality standards, they may have significant joint toxic effects on...

Claims

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Application Information

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IPC IPC(8): A01K61/10G06Q50/02G01N33/00
CPCA01K61/10G01N33/00G06Q50/02G01N33/5014G01N33/5088G01N2333/4603
Inventor 王彦华王菲迪王新全柳新菊杨桂玲安雪花王强
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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