The invention provides a vacuum ampoule sealing machine, and relates to the technical field of biochemical experiment equipment. The vacuum ampoule sealing machine mainly comprises a rotary type vacuum connector, a rotary drawing ampoule or a test tube clamp, a lifting bracket and a sealing and heating device. The vacuum ampoule sealing machine has the advantages that the vacuum ampoule sealing machine overcomes the defects of the prior art, has the characteristics of simple structure, convenient operation, high efficiency, good sealing quality and high qualification rate (as high as 100 percent), is particularly suitable for long-term oxygen isolation sealing preservation of little biochemical products or samples which are easily oxidized, decomposed, denatured and inactivated in air and frozen and dried in an ampoule or a test tube, such as microbe strains, animal and plant cells or tissues, vaccine, viable bacteria preparation, enzyme preparation, active protein, blood products, flavor food, health-care food and biochemical drugs, and suitable for vacuum sealing preservation of all oxygen-sensitive biological and chemical substances.