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Botanical attractant for Chouioia cunea Yang as natural enemy of fall webworm

A technology of Chou's larvae and American white moth, which can be applied in the fields of attracting pests, plant growth regulators, plant protection, etc.

Pending Publication Date: 2022-04-22
TIANJIN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical control adopts methods such as removing egg masses, cutting off nets, surrounding grass to induce pupae, etc., but because it is time-consuming and laborious, it is only suitable for sporadic occurrences and areas where the damage is not serious.
Chemical control is relatively effective, but the improper use of chemical pesticides will not only pollute the environment, but may also lead to the continuous increase of resistance of pests and diseases, and at the same time kill natural enemies, reducing their long-term natural control ability of pests

Method used

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  • Botanical attractant for Chouioia cunea Yang as natural enemy of fall webworm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of attractants:

[0031] Reagents: tridecane, undecane, tetradecane, hexadecane, nonadecane and hexadecane were purchased from Aladdin Reagent Company.

[0032] Preparation of tridecane: Take 13.2 μl stock solution and add 986.8 μl n-hexane to prepare 10 mg / ml stock solution; dilute 1000 times with n-hexane to obtain 10 μg / ml (10ng / μl) working solution for later use.

[0033] Preparation of undecane: Take 13.5 μl stock solution and add 986.5 μl n-hexane to prepare 10mg / ml stock solution; dilute 1000 times with n-hexane to obtain 10μg / ml (10ng / μl) working solution for later use.

[0034] Tetradecane preparation: take 13.1 μl stock solution and add 986.9 μl n-hexane to prepare 10mg / ml stock solution; dilute 1000 times with n-hexane to obtain 10μg / ml (10ng / μl) working solution for later use.

[0035] Preparation of hexadecane: take 12.9 μl stock solution and add 987.1 μl n-hexane to prepare 10mg / ml stock solution; dilute 1000 times with n-hexane to obtain 10μg...

Embodiment 2

[0040] Insects to be tested:

[0041] Zhou's gnat was donated by Yuzhongnan Forestry Pest Natural Enemy Breeding Research Center, Luohe City, Henan Province, and subcultured in the Key Laboratory of Animal and Plant Resistance of Tianjin Normal University. Culture conditions: artificial climate chamber (PQX-350H), temperature 25°C, relative humidity 75%, photoperiod 13L:11D. After the adults were obtained, they were inoculated into tussah silkworm chrysalis, subcultured under the same conditions, and adult bees within 24 hours of emergence were selected for use in the experiment.

Embodiment 3

[0043] Behavioral experiment of four-arm olfactometer

[0044] Four-arm olfactometer (Model QT-WII01)

[0045] Control: take 10 μ lddH 2 O is evenly added dropwise to the quantitative filter paper (2cm long, 1cm wide).

[0046] n-Hexane: Take 10 μl of n-hexane and drop evenly onto the quantitative filter paper (2cm long, 1cm wide).

[0047] Mixed compounds: take 10 μl each of tridecane, undecane, tetradecane, hexadecane, nonadecane, and hexadecane solutions (10ng / μl), mix well, and drop 10 μl of them evenly onto the quantitative filter paper (Length 2cm, width 1cm).

[0048] Mixed compound without tridecane: Take 10 μl each of undecane, tetradecane, hexadecane, nonadecane, and hexadecane solutions (10ng / μl), mix well, and drop 10 μl evenly onto the quantitative filter paper (Length 2cm, width 1cm).

[0049] Connect the experimental equipment in the order of air pump—activated carbon drying tower—distilled water washing cylinder—four-arm olfactometer. Place the control fi...

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Abstract

The invention discloses an attractant for a parasitic natural enemy Chouioia cunea Yang of fall webworms. Volatile matters of leaves of platanus orientalis, paulownia, purple-leaf plum, poplar and mulberry before and after hyphantria cunea eats are collected through a headspace collection method, and a group of compounds are obtained through analysis by adopting a gas chromatograph-mass spectrometer (GC-MS) and combining an antennopotential-gas chromatograph (GC-EAD) technology. Four-arm olfactometer behavioral experiments find that the mixed compound (tridecane, undecane, tetradecane, hexadecane, nonadecane and heneicosane) has an obvious attraction effect on Chouioia cunea Yang, and the attraction effect is obviously reduced after the tridecane is removed from the mixed compound. The taxis of the Chouioia cunea Yang which is a natural enemy of the fall webworm to the mixed compound is researched through indoor behavioral experiments, key information compounds capable of attracting the Chouioia cunea Yang are screened, and a theoretical basis is provided for research and development of attractants, improvement of the parasitic rate of the Chouioia cunea Yang and better utilization of the Chouioia cunea Yang for prevention and treatment of the fall webworm.

Description

[0001] This invention was funded by the National Natural Science Foundation of China (31702058) and Tianjin Higher Education Science and Technology Development Project (2019KJ089). technical field [0002] The invention belongs to the field of plant protection, and relates to the screening and application of key information compounds capable of attracting the natural enemy of the white moth, the natural enemy of the white moth, Chow's, and more specifically a botanical attractant for the natural enemy of the white moth, the natural enemy of the small wasp. Background technique [0003] The American white moth (Hyphantriacunea Drury) is one of the important pests and diseases that seriously threaten my country's agricultural and forestry production. It can cause serious damage to most broad-leaved trees such as paulownia, paulownia, poplar, mulberry, and ash. cause serious harm. [0004] The common control methods of American white moth include physical control, chemical contr...

Claims

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Application Information

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IPC IPC(8): A01N27/00A01P19/00A01G13/00
CPCA01N27/00A01G13/00
Inventor 潘丽娜金欣高雯芳胡鹏飞刘新宇覃东玉任睿张天天李敏
Owner TIANJIN NORMAL UNIVERSITY
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