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Kit for quickly detecting fall webworms

A kind of American white moth, kit technology, applied in the direction of microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of difficulty in distinguishing, difficult to make fast and accurate identification, etc., to achieve convenient operation, good economic benefits and Social effect, good practical effect

Inactive Publication Date: 2013-12-18
SUZHOU CITY INVASIVE PEST PREVENTION & CONTROL TECH CENT
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It is extremely difficult to distinguish the adult larvae of the American white moth from other species of the family Mothidae in morphology, especially when the eggs or incomplete worms of the American white moth are intercepted during port quarantine, it is more difficult to make a rapid and accurate identification by using traditional morphological classification

Method used

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  • Kit for quickly detecting fall webworms
  • Kit for quickly detecting fall webworms
  • Kit for quickly detecting fall webworms

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Embodiment 1

[0029] The kit for the method for rapid detection of white moth, at least includes the specific primer COI01 / 02 or SpmR01 / 02 reagent in more than one dosage. When in use, it can be purchased additionally: Taq buffer, MgCl 2 , dNTP mix, Taq DNA polymerase, ddH 2 O, can be used together. Among them, ddH 2 O can be prepared by yourself.

[0030] At the same time, the kit for the method of rapid detection of white moth can be directly prepared with all the solutions, specifically composed of the following reagents in more than one dosage: 10×Taq buffer, 2 mmol / L MgCl 2 , 200 μmol / L dNTP mix, Taq DNA polymerase, specific primer COI01 / 02 or SpmR01 / 02, ddH2O.

[0031] Among them, the sequences of specific primers COI01 / 02 and SpmR01 / 02 are as follows:

[0032] COI01 sequence: 5'-CAGGAACTGGATGAACA-3',

[0033] COI02 sequence: 5'-CCTACTGCTCATACGAA-3',

[0034] SpmR01 sequence: 5'-ACGCTGTTGTCCGTATTT-3',

[0035] SpmR02 sequence: 5'-CTGATTTCTTCGGTGTTG-3'.

[0036] When selecting...

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Abstract

The invention discloses a kit for quickly detecting fall webworms, which at least comprises more than 1 dose of specific primers COI01 / 02 or SpmR01 / 02 reagents, wherein the sequence of the specific primer COI01 is 5'-CAGGAACTGGATGAACA-3', and the sequence of the specific primer COI02 is 5'-CCTACTGCTCATACGAA-3'; and the sequence of the SpmR01 is 5'-ACGCTGTTGTCCGTATTT-3', and the sequence of the SpmR02 is 5'-CTGATTTCTTCGGTGTTG-3'. The kit disclosed by the invention is an efficient accurate convenient fall webworm molecule detection technique, can be used for distinguishing fall webworms from other types of Arctiidae on the molecular level, can satisfy the use demands, has favorable practicality, and can generate favorable economic benefit and social effect.

Description

technical field [0001] The invention belongs to the technical field of detection of the white moth, and in particular relates to a kit for rapidly detecting the white moth. Background technique [0002] American white moth [Hyphantria cunea (Drury)], also known as autumn curtain caterpillar, autumn curtain moth, belongs to Lepidortera (Lepidortera), lamp moth family (Arctiidae), lamp moth subfamily (Arctiinae), is a world-renowned quarantine pest biology. Originated in North America, distributed in the United States, Canada, Mexico and other regions, it was introduced to Europe in 1940, Japan in 1945, South Korea in 1958, and Dandong, Liaoning, my country in 1979. At present, the domestically announced epidemic areas include Beijing, Tianjin, Hebei, Shandong, Liaoning, Shaanxi, Jiangsu and other provinces and cities. The General Office of the State Council has issued documents many times, requiring vigorous efforts to strengthen the prevention and control of white moths in...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 詹国辉陈景云郑斯竹高渊陈云芳邓海娟赵毓郎葛华林
Owner SUZHOU CITY INVASIVE PEST PREVENTION & CONTROL TECH CENT
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