American white moth artificial breeding and propagating method

A technology of American white moth and artificial feed, which is applied in the field of artificial feeding and subculture of American white moth, can solve the problems that affect the growth of American white moth, pass on generations, cannot reproduce continuously, and is difficult to produce on a large scale.

Inactive Publication Date: 2009-12-02
INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] To produce the insecticide against the NPV, a large number of larvae must be raised to reproduce the virus. At present, natural feeds are mostly used in the wild, which will be restricted by many external environmental factors, such as: climate, region, and insect population. Density, tree height, terrain, etc. will directly affect the growth and generation of the American white moth. Since the American white moth survives the winter in the wild for about 6 months, it cannot reproduce continuously and it is difficult to carry out large-scale production.

Method used

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  • American white moth artificial breeding and propagating method

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Experimental program
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Effect test

Embodiment 1

[0036] Select 50 pairs of white moth adults, lay 5,750 eggs in total, and perform routine disinfection; at 25°C, hatch 5,175 larvae, prepare artificial feed for white moth larvae according to formula 1 in Table 1, heat and mix, and distribute to each In the American white moth larva rearing container, insert the newly hatched larvae, put them into a healthy insect breeding room and raise them, keep the temperature at 22-25°C, and after 25 days, 4709 pupae will be formed, and the pupae will be put into the adult worm-laying room for 10 days. A total of 4,049 adults emerged from Tin Hau, including 2,200 females and 1,849 males. These adult males and females were matched into 1,849 pairs, and a total of 248,600 eggs were laid. During the egg-laying cycle, 223,740 larvae were hatched, and 203,603 pupae were produced, and the amount of eggs laid was countless.

Embodiment 2

[0038] Select 50 pairs of white moth adults, lay 6150 eggs, and hatch 5596 larvae under the condition of 25°C, make artificial feed for white moth larvae according to formula 2 in Table 1, heat and mix them, and pack them into white moth larvae for feeding Put the newly hatched larvae into the container and raise them in a healthy insect breeding room. After 26 days, 5092 pupae became pupae, and the pupae were placed in the adult room. After 11 days, a total of 4633 adults emerged, including 2709 females and 1924 males. There were 1924 pairs of larvae, 311535 eggs were laid, and 283496 larvae were hatched and 257982 pupae were hatched in 10 days.

Embodiment 3

[0039] Embodiment 3, 50 pairs of white moth adults, lay eggs 6400, hatch 5888 larvae under the condition of 25 ℃, make artificial feed for white moth larvae according to formula 3 in Table 1, heat and mix, and pack into white white moth. In the moth larva breeding container, the newly hatched larvae were inserted and put into a healthy insect breeding room to raise them. After 25 days, 5594 pupae became pupae. After 10 days, the pupae were placed in the adult room, and 4866 adults emerged, including 2630 females and 2630 males. There were 2,236 worms, 328,750 eggs were laid, the egg period was 9 days, 322,175 larvae were hatched, and 306,067 larvae became pupae.

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Abstract

A kind of artificial feeding and subculture method of American white moth, the method is as follows: 1) routinely sterilize the eggs of American white moth; put into a healthy insect breeding room to raise; the components of the feed and their weight percentages are as follows: germ 5-12; sucrose 2-5; protein 3-7; Webster's salt 0.3-1; sorbic acid 0.4-1.2 ; methyl p-hydroxybenzoate 0.1-0.5; ascorbic acid 0.02-0.19; vitamin B 0.03-0.15; agar 1.1-1.8; cholesterol 0.05-0.16; water 75-85; , eclosion of adults at room temperature and removal of diapause; 4) matching the male and female adults after eclosion into a pair, and putting them into the adult oviposition room for mating and oviposition. Among them, the room temperature for adult eclosion and release of diapause at room temperature is 25 degrees, and the cleanliness of the healthy insect breeding room should be 1,000 degrees, and the room temperature should be kept at 22-25 degrees. It is not restricted by the external environment, and can provide sufficient insect sources for the large-scale continuous production of safe and efficient biopesticides.

Description

technical field [0001] The invention relates to a method for artificial breeding and subculture of the white moth. Background technique [0002] The American white moth (Hyphanlria cunea) is a devastating pest and has been listed as an international quarantine object. Domestically distributed in Liaoning, Shandong, Tianjin, and parts of Shaanxi and Hebei, they are currently threatening Beijing directly. The American white moth has a very mixed diet, mainly harming fruit trees, street trees, ornamental trees and other broad-leaved trees and crops. There are more than 100 kinds of broad-leaved trees in the United States; 230 species of plants in Europe; and 317 species in Japan. Only in the occurrence area of ​​American white moth in Liaoning Province, my country, more than 100 kinds of plants were damaged, and sugar maple, ash, mulberry, and cherry trees were the most affected trees, followed by poplar, willow, sycamore, clove, elm, five-leaf maple, Larix, etc.; apples, hawt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D19/00A01K67/00
Inventor 张永安王玉珠徐保泯曲良建
Owner INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY
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