35 results about "Chitin synthase" patented technology

Methods and means are provided for the modification of the reactivity of plant secondary cell walls, particularly in cotton cell walls found in cotton fibers. This can be conveniently achieved by expressing a chimeric gene encoding a Saprolegnia monoica chitin synthase in cotton plants.

The invention discloses a toxoptera citricida chitin synthase gene. The sequence of the toxoptera citricida chitin synthase gene is shown as SEQ ID NO:3. The invention further discloses dsRNA of the toxoptera citricida chitin synthase gene, and the sequence of the dsRNA is shown as SEQ ID NO:6. The invention further discloses a synthesis method of the dsRNA of the toxoptera citricida chitin synthase gene. The synthesis method includes the following steps that total RNA of toxoptera citricida is extracted and subjected into reverse transcription to form cDNA serving as an amplification template; PCR amplification is carried out through primers with the sequences of SEQ ID NO:4 and SEQ ID NO:5, a product is recycled after electrophoresis, and the dsRNA of the toxoptera citricida chitin synthase gene is synthesized with a gel recycling product as a template. According to the dsRNA of the chitin synthase gene, gene silencing efficiency is high, toxoptera citricida has obvious phenotypic changes after gene interference, and the good application prospects are achieved in the aspect of researching and developing novel pesticide.

The invention relates to chitin synthase in phytophthora capsici. The amino acid sequence of the chitin synthase has the similarity above 90%, preferably above 95% and more preferably above 98% with an amino acid sequence represented by SEQ ID No. 4 and has same functions with the amino acid sequence represented by SEQ ID No. 4. By virtue of the activity level of the chitin synthase of the phytophthora capsici, the yields of active sporangia and active zoospores can be adjusted, so that the pathogenicity of the phytophthora capsici or the mobidity extent of a host is influenced.

The invention relates to a target fungus cytoderm chitin synthetase decreasing agent high-throughput screening model, belonging to the field of genetic engineering, the model consists of a UMcsh5 gene promoter sequence UMPRO, a recombinant plasmid Ppic9KG-UMPRO-LUC, monoclonal cell strain GS115-UMPRO-LUC for stable expression of the UMPRO promoter, a screening reagent and a luciferase reporter gene detection system. The screening model has the advantages of simple and convenient operation, low cost, high flexibility, wide applicable range, high screening efficiency and the like and has great application potentiality.

The present invention discloses 1-oxo-2,8-diazaspiro[4.5]decane piperidine carboxylic acid amide compound and its preparation method and application. The structure of said compound is shown in general formula 1: where R 1 is hydrogen; R 2 For: various substituted aromatic amines. It has been proved by biological activity test experiments that some compounds have certain inhibitory activity against gram-positive bacteria, gram-negative bacteria and fungi, and have obvious inhibitory activity against chitin synthase, with good antibacterial effect, and can be used to prepare anti-pathogenic Microbial medicine. Moreover, the preparation raw material is simple, cheap and easy to obtain, and the application in the aspect of anti-infection is of great significance.

The invention provides an insect chitin synthase 1A gene segment, dsRNA and application thereof. The dsRNA is synthesized by the chitin synthase 1A gene segment with the sequence of SEQ ID NO:1 which is obtained by the gene sequence of the chitin synthase 1A of Locusta migratoria manilensis with the accession number of GU067730 in an NCBI database. After the dsRNA is injected in body cavities of insects, the insects die because of difficult moulting. A new way for developing a safe and pollution-free method for preventing and controlling insect is provided.

The invention discloses the application of chitin synthase CHSA‑2b / 19b of lepidopteran insects in pest control. The invention discovers malformed wing veins of insects by fully injecting dsRNA that interferes with exon 2b and 19b of lepidopteran insects. Wrinkled and thin, wings smaller, indicating that transcripts containing 2b and / or 19b affect insect wing development. Artificial substances that inhibit the expression of chitin synthase transcripts containing 2b and / or 19b in lepidopteran insects or compounds screened from plants can be used as inhibitors, such as injecting dsRNA of CHSA-2b to lepidopteran insects, The purpose of pest control can be achieved. Similarly, substances that promote the degradation of chitin synthase transcripts containing 2b and / or 19b in Lepidoptera insects, or substances that inhibit the normal function of chitin synthase transcripts containing 2b and / or 19b As a pest insecticide, it can achieve the purpose of pest control.