Insect chitin synthase 1A gene segment, dsRNA and application thereof

A chitin synthase, gene fragment technology, applied in DNA/RNA fragment, application, recombinant DNA technology and other directions, can solve the problems of increased dosage, increased control cost, long insecticidal time, etc.
CN101831447BInactive Publication Date: 2011-09-07SHANXI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SHANXI UNIV
Publication Date
2011-09-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides an insect chitin synthase 1A gene segment, dsRNA and application thereof. The dsRNA is synthesized by the chitin synthase 1A gene segment with the sequence of SEQ ID NO:1 which is obtained by the gene sequence of the chitin synthase 1A of Locusta migratoria manilensis with the accession number of GU067730 in an NCBI database. After the dsRNA is injected in body cavities of insects, the insects die because of difficult moulting. A new way for developing a safe and pollution-free method for preventing and controlling insect is provided.
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Description

technical field

[0001] The present invention relates to the field of biotechnology. It specifically relates to an insect chitin synthase 1A gene fragment and its dsRNA and the application of the dsRNA in killing pests. Background technique

[0002] The damage caused by agricultural pests is an important factor restricting agricultural production in my country. Long-term application of chemical insecticides leads to a series of problems: 1) Insects become resistant to insecticides, the dosage of pesticides increases, and the cost of control increases; 2) Pesticide residues lead to serious environmental pollution; 3) Has a greater impact on non-target organisms. Existing biopesticides are widely used in pest control, but the insecticidal time is long and the effect is slow.

[0003] RNA interference (RNAi) is a specific post-transcriptional gene silencing phenomenon caused by double-stranded RNA molecules, which won the Nobel Prize in 2006. The discovery of RNAi not only pro...

Claims

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