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Chitin synthase of phytophthora capsici as well as gene and application of chitin synthase

A technology of Phytophthora capsici and chitin synthase, applied in the field of chitin synthase, can solve problems such as economic losses

Active Publication Date: 2016-08-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] There are many important plant pathogenic oomycetes in Oomycota, which can infect a variety of host plants. For example, Phytophthora capsici can infect peppers and tomatoes in Solanaceae, green beans and kidney beans in leguminous plants and Most plants in the Cucurbitaceae cause serious economic losses

Method used

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  • Chitin synthase of phytophthora capsici as well as gene and application of chitin synthase
  • Chitin synthase of phytophthora capsici as well as gene and application of chitin synthase
  • Chitin synthase of phytophthora capsici as well as gene and application of chitin synthase

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Experimental program
Comparison scheme
Effect test

Embodiment

[0046] 1. Cloning of Phytophthora capsici chitin synthase (PCCHS) gene

[0047] 1.1 Extraction of total RNA from Phytophthora capsici

[0048] Phytophthora capsici strain LT1534 was cultured on solid medium PDA in the dark at 25°C for 4 days. A 5mm diameter cake was taken from the edge of the colony and inoculated on a cellophane PDA plate. After 4 days of dark culture at 25°C, the bacteria were scraped For silk, take about 30mg of mycelium. As for the 2ml centrifuge tube without RNase, add two steel balls with a diameter of 5mm. After freezing in liquid nitrogen, use a ball mill to grind to powder. Use Promega's SV Total RNA Extraction Kit to extract Phytophthora sojae RNA. Refer to the plant tissue RNA extraction instructions in the kit for specific method steps.

[0049] 1.2 Synthesis of the first-strand cDNA reverse transcription of Phytophthora capsici

[0050] The first-strand cDNA synthesis uses Takara’s RT reagent Kit with gDNAEraser (Perfect Real Time) kit. Specific steps ...

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PUM

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Abstract

The invention relates to chitin synthase in phytophthora capsici. The amino acid sequence of the chitin synthase has the similarity above 90%, preferably above 95% and more preferably above 98% with an amino acid sequence represented by SEQ ID No. 4 and has same functions with the amino acid sequence represented by SEQ ID No. 4. By virtue of the activity level of the chitin synthase of the phytophthora capsici, the yields of active sporangia and active zoospores can be adjusted, so that the pathogenicity of the phytophthora capsici or the mobidity extent of a host is influenced.

Description

Technical field [0001] The present invention relates to chitin synthase, in particular to the chitin synthase in Phytophthora capsici. Background technique [0002] There are many important phytopathogenic oomycetes in Oomycota, which can infect a variety of host plants. For example, Phytophthora capsici can infect solanaceous plants peppers and tomatoes, legumes green beans and kidney beans, and Most plants in the Cucurbitaceae cause severe economic losses. Among them, pepper blight is a devastating oomycete disease in pepper planting and production. It occurs in most areas of my country. The pepper blight has the characteristics of multiple infection routes, short course of disease, and rapid spread. Inside, it can cause a large number of peppers to wilt and die, and even die. Soybean root rot caused by Phytophthora sojae infection is one of the important diseases that seriously endanger soybean production, causing economic losses of more than 1 billion US dollars every year. ...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N15/54C12Q1/68C12N15/11
CPCC12N9/1051C12Q1/6876C12Q2600/166C12Y204/01016
Inventor 刘西莉张灿王为镇刁永朝牟文君刘利迟源冬刘鹏飞张文华
Owner CHINA AGRI UNIV
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