Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Chitin synthase from Phytophthora capsici and its gene and application

A technology of Phytophthora capsici and chitin synthase, applied in the field of chitin synthase, can solve problems such as economic losses

Active Publication Date: 2019-12-06
CHINA AGRI UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] There are many important plant pathogenic oomycetes in Oomycota, which can infect a variety of host plants. For example, Phytophthora capsici can infect peppers and tomatoes in Solanaceae, green beans and kidney beans in leguminous plants and Most plants in the Cucurbitaceae cause serious economic losses

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Chitin synthase from Phytophthora capsici and its gene and application
  • Chitin synthase from Phytophthora capsici and its gene and application
  • Chitin synthase from Phytophthora capsici and its gene and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0046] 1. Cloning of Phytophthora capsici chitin synthase gene PCCHS (Phytophthora capsici chitin synthase)

[0047] 1.1 Extraction of Phytophthora capsici total RNA

[0048] Phytophthora capsici strain LT1534 was cultivated in the dark at 25°C for 4 days on the solid medium PDA, and the bacteria cake with a diameter of 5mm was picked from the edge of the colony and inoculated on the PDA plate covered with cellophane, and the bacteria were scraped after 4 days of dark culture at 25°C. For silk, take about 30 mg of mycelium, put it into a 2ml centrifuge tube without RNase, and add two steel balls with a diameter of 5mm, freeze it in liquid nitrogen and grind it to powder with a ball mill. Phytophthora sojae RNA was extracted using the SV Total RNA extraction kit from Promega, and the specific method steps refer to the instructions for plant tissue RNA extraction in the kit.

[0049] 1.2 Synthesis of first-strand cDNA by reverse transcription of Phytophthora capsici

[0050] F...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention relates to chitin synthase in phytophthora capsici. The amino acid sequence of the chitin synthase has the similarity above 90%, preferably above 95% and more preferably above 98% with an amino acid sequence represented by SEQ ID No. 4 and has same functions with the amino acid sequence represented by SEQ ID No. 4. By virtue of the activity level of the chitin synthase of the phytophthora capsici, the yields of active sporangia and active zoospores can be adjusted, so that the pathogenicity of the phytophthora capsici or the mobidity extent of a host is influenced.

Description

technical field [0001] The present invention relates to chitin synthase, in particular chitin synthase in Phytophthora capsici. Background technique [0002] There are many important plant pathogenic oomycetes in Oomycota, which can infect a variety of host plants. For example, Phytophthora capsici can infect peppers and tomatoes in Solanaceae, green beans and kidney beans in leguminous plants and Most plants in the Cucurbitaceae cause serious economic losses. Among them, pepper blight is a devastating oomycete disease in pepper planting and production, which occurs in most parts of my country. Pepper blight has the characteristics of multiple infection routes, short course of disease, and fast spreading speed. When the onset conditions are suitable, short-term It can cause a large number of wilting and death of peppers, or even extinction. Soybean root rot caused by Phytophthora sojae infection is one of the important diseases that seriously endanger soybean production, ca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/10C12N15/54C12Q1/6876C12N15/11
CPCC12N9/1051C12Q1/6876C12Q2600/166C12Y204/01016
Inventor 刘西莉张灿王为镇刁永朝牟文君刘利迟源冬刘鹏飞张文华
Owner CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com