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Application of banana fusarium oxysporum 4 # physiological race chitin synthase 6 gene

A technology of banana fusarium wilt and physiological race, which is applied in the field of molecular biology to achieve the effect of improving pathogenicity

Pending Publication Date: 2022-08-05
ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The formation mechanism of Fusarium oxysporum cuba-specific chlamydospores has not been reported, and there are even few reports on the formation mechanism of chlamydospores in filamentous fungi. Only Ohara&Tsuge reported that F. oxysporum f .sp.melonis) FoSTUA gene can inhibit the formation of chlamydospores to a certain extent

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  • Application of banana fusarium oxysporum 4 # physiological race chitin synthase 6 gene
  • Application of banana fusarium oxysporum 4 # physiological race chitin synthase 6 gene
  • Application of banana fusarium oxysporum 4 # physiological race chitin synthase 6 gene

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Embodiment Construction

[0033] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0034] Example:

[0035] 1 Materials and methods

[0036] 1.1 Materials

[0037] 1.1.1 Experimental strains, vectors and banana varieties

[0038] Banana Fusarium wilt No. 4 physiological race (F.oxysporum f.sp.cubense race 4, Foc4), identified and preserved by the laboratory. The gene complementing vector pYF11 and the yeast XK125 strain were provided by Professor Zhang Haifeng of Nanjing Agricultural University, and the banana variety inoculated in the experiment was Brazilian banana (Cavendish, AAA).

[0039] 1.1.2 Experimental reagents

[0040] A 100 mg / mL stock solution was prepared with dimethyl sulfoxide (DMSO). Fluorescent whitening agent (Calcofluor white, CFW) was purchased from Sigma-aldrich (Beijing), Congo Red (CR), MgCO 3 Purchased from Beijing Baierdi Biotechnology Co., Ltd., Taq DNA pol...

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Abstract

The invention discloses an application of a banana fusarium oxysporum 4 # physiological race (chitin synthase 6) gene. The invention further discloses a preparation method of the banana fusarium oxysporum 4 # physiological race. Specifically, the invention provides the application of the banana wilt pathogen No.4 physiological race CHS6 gene in the pathogen resistance, pathogenicity and chlamydospore formation process. The CHS6 gene can improve pathogenicity of pathogenic bacteria and regulate and control formation of chlamydospores. The research result provided by the invention can provide a basis for the research on the drug resistance and pathogenic mechanism of the fusarium oxysporum to the phenamacril fungicides.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to the use of the chitinsynthase 6 gene of Fusarium wilt No. 4 physiological species. Background technique [0002] Banana fusarium wilt is a devastating soil-borne disease that destroys banana vascular bundles and leads to plant death. The race with the strongest pathogenicity; Foc4 has a strong saprophytic ability and can survive in the soil for a long time, which is a typical latent infection. Banana fusarium wilt has caused serious harm and huge economic losses to the global banana industry and banana growers. [0003] Chitin is widely present in the cell wall of pathogenic fungi and is an essential component of the fungal cell wall. Chitin synthase (CHS) is a key enzyme in chitin biosynthesis. There are many CHSs, and different kinds of CHSs may play different roles in different fungi. Researches on the biological functions of CHS mainly focus on model strains suc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/54C12N15/80C12N3/00A01G7/06A01G13/00
CPCC12N9/1051C12N15/80C12N3/00C12Y204/01016A01G7/06A01G13/00
Inventor 彭军曾凡云漆艳香王艳玮张欣丁兆建谢艺贤
Owner ENVIRONMENT & PLANT PROTECTION INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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