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Method for preventing and treating corn field armyworms

A technology for cornfield and armyworm, which is applied in the field of armyworm control and cornfield armyworm control, can solve the problems such as lagging research on armyworm insecticidal activity, achieve shortening the time required for prevention and control, good application prospects, and high activity Effect

Inactive Publication Date: 2019-03-08
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In China, the research on the insecticidal activity of Bt against armyworm is relatively lagging behind. Only a few reports by Wang, Jiang Shanjun and Bai Xuefeng found that Cry1Ab, Cry1Ac, and Cry2Ac proteins have insecticidal activity against Oriental Armyworm.

Method used

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  • Method for preventing and treating corn field armyworms
  • Method for preventing and treating corn field armyworms
  • Method for preventing and treating corn field armyworms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Pathogenicity of entomopathogenic nematode HB to armyworm

[0029]Add 30g of sterilized soil (humidity about 18%, pass through a 40 mesh sieve) into a 9.5cm glass culture dish, put 20 healthy, uniform 3rd instar armyworm larvae, and put in an appropriate amount of fresh corn leaves as feed; Dilute the HB nematode infection stage larvae with sterile water into six concentration gradients of 20IJs / mL, 30IJs / mL, 50IJs / mL, 75IJs / mL, 100IJs / mL, and 125IJs / mL; take 1mL nematode suspension for each concentration Evenly drop into the Petri dish. Three replicates were set up for each concentration, and sterile water was used as a control. Each treatment was placed in an incubator with a constant temperature of 25°C and a relative humidity of 80%. Add corn leaves in time as needed, observe and record the death of armyworm every 24h, and count after the 6th day of nematode infection, the results are obtained by figure 1 Shown: The death rate of armyworm increases with the incre...

Embodiment 2

[0031] Determination of Toxicity of Bt Strain HD1 to Armyworm

[0032] Dilute the quantified Bt strain HD1 with sterile water to 1.5×10 11 CFU / mL, 3×10 11 CFU / mL, 1.5×10 12 CFU / mL, 3×10 12 CFU / mL, 1.5×10 13 CFU / mL, 3×10 13 CFU / mL six concentration gradients. Add about 30g of sterilized soil (humidity about 18%, pass through a 40-mesh sieve) into a glass petri dish with a diameter of 9.5cm, soak the fresh corn leaves in the bacterial suspension of each concentration for about 1min, dry naturally, and take an appropriate amount Put them into petri dishes, and insert 20 healthy, uniform 3rd instar armyworm larvae into each dish. Each treatment was repeated 3 times while soaking in sterile water as a control. Each treatment was placed in an incubator with a constant temperature of 25°C and a relative humidity of 80%. Add in time the maize leaf after soaking bacteria solution and dry as required, and observe and record the death situation of armyworm every 24h. The results...

Embodiment 3

[0034] Toxicity determination of Bt strain G03 to armyworm

[0035] Assay method is the same as in Example 2, and the Bt bacterial strain G03 quantified is diluted to 0.5 × 10 with sterile water. 11 CFU / mL, 1×10 11 CFU / mL, 5×10 11 CFU / mL, 1×10 12 CFU / mL, 5×10 12 CFU / mL, 1×10 13 CFU / mL six concentration gradients, the indoor toxicity test results of Bt strain G03 to armyworm are as follows Figure 4 Shown: With the increase of Bt concentration, the death rate of armyworm gradually increased, 5.0×10 12 The CFU / mL concentration of Bt showed a slow increase in the death rate of armyworm after 3 days of treatment (P>0.05), and the death rate of armyworm reached 96.67%, 1.0×10 13 Under the high concentration of CFU / mL, the death rate of armyworm reached 100%; 5.0×10 11 CFU / mL, 1.0×10 12 The CFU / mL concentration of Bt showed a slow increase in armyworm mortality after 4 days of treatment (P>0.05), and the armyworm mortality rate reached 80%; 0.5×10 11 After 4 days of treatm...

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Abstract

The invention discloses a method for preventing and treating corn field armyworms. A bacteria solution of Bt strain HD1 or Bt strain GO3 is sprayed and applied to a corn field with corns growing to the flare opening stage; after the Bt strain bacteria solution is sprayed and applied for 2-3 days, an entomopathogenic nematode Hb nematode suspension is sprayed and applied. The method for preventingand treating the corn field armyworms, disclosed by the invention, has the advantages that a coordinated synergistic effect on the prevention and treatment of the armyworms is realized through combined use of entomopathogenic nematode Hb nematodes and the Bt strain HD1 or the Bt strain GO3, the pathogenicity to the armyworms can be obviously enhanced, the prevention and control time can be shortened, and the application prospect can be good.

Description

technical field [0001] The invention relates to a method for preventing and controlling armyworm, in particular to a method for preventing and controlling armyworm in cornfield, and belongs to the field of plant protection. Background technique [0002] Armyworm [Mythimna separata (Walker)] is an insect of Lepidoptera Noctuidae. It has the characteristics of wide occurrence range, many generations of damage, and long history of damage. It can feed on more than 100 kinds of plants in 16 families. The bare plant leaves can also eat the ears of gramineous crops, which poses a serious threat to food safety production. [0003] Due to the influence of factors such as crop planting, climatic conditions, and farmland habitats, the occurrence of armyworms in my country has changed significantly among crops, regions, and generations. Corn has become the most harmful crop. For a long time, the chemical control of armyworms has produced many negative effects, such as increased drug res...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P7/04
CPCA01N63/10
Inventor 尹姣李克斌张帅李而涛曹雅忠梅向东
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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