A strain of Beauveria bassiana and its application
A technology of Beauveria bassiana and spores, which can be used in applications, fungi, biocides, etc., can solve problems such as environmental pollution, tea aphid resistance, and affect the quality of tea, so as to reduce the amount of use and the pathogenicity synergistic effect
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Embodiment 1
[0093] (1) Screening of pathogenic strains
[0094] (1) Beauveria bassiana isolated from aphids and Hemiptera insects provided by the Entomogenic Fungi Research Center of the Key Laboratory of Microbial Control of Anhui Agricultural University was selected for virulence screening; the basic information of the strains is shown in Table 1;
[0095] Table 1 is the source and collection place of Beauveria bassiana
[0096]
[0097] (2) Preparation of spore suspension of Beauveria bassiana: the strain was inoculated on the prepared SDAY medium, placed in an artificial intelligence light incubator (photoperiod 14L:10D) at 25°C for 10 days, and waited for sufficient spore production. Prepare a 50ml Erlenmeyer flask and fill it with 20ml TW-80 solution with a concentration of 0.05%. After sterilization and cooling, use a spore shovel to scrape the spore powder on the medium into it, and shake it on a vortex magnetic oscillator at a frequency of 150r / min for 15min , the spores are ...
Embodiment 2
[0114] (1) Effect of culture medium on sporulation of Beauveria bassiana Bb2032
[0115] (1) Use a 0-100ul adjustable pipette gun to draw 100ul with a concentration of 1×10 6 The spore suspension of highly pathogenic bacterial strains with spores / ml was received on each dish, and the spore suspension was evenly coated with a coating rod, and after standing for 2 minutes, it was sealed with a parafilm and placed in a 25°C artificial intelligence light incubator (light Period 14L: 10D) cultured for 10 days, each medium was a treatment, and each treatment was repeated five times. After cultivation, five holes were punched in each dish with a puncher with a diameter of 4 mm, and the punching positions were the middle and the middle to the middle. At 1 / 2 of the line connecting the edges of the four directions of the dish, put the removed bacteria together with the culture medium into an Erlenmeyer flask filled with 30ml of TW-80 solution with a concentration of 0.05%. Vibrate at a...
Embodiment 3
[0125] (1) Effect of culture temperature on spore germination of Beauveria bassiana Bb2032
[0126] (1) Prepare water agar (WA) medium (dissolve 20g of agar in 1000ml of water, sterilize at 120°C for 20min, add 2ml per dish into a disposable sterile petri dish (D=40mm) when cooled to about 50°C cooling, standby);
[0127] (2) Use a 0.2-2ul adjustable pipette to draw 1ul with a concentration of 1×10 6 The spore suspension of highly pathogenic strains with spores / ml was planted in the middle of the petri dish, sealed with parafilm after standing for 2 minutes, and placed in 18°C, 22°C, 25°C, 28°C, 30°C, 35°C respectively In the artificial intelligence constant temperature incubator, each temperature is a treatment, and each treatment has four groups of repetitions;
[0128] (3) When cultured for 12h and 24h, the spore germination rate of 2 replicates was counted for each treatment respectively. When using an inverted microscope, remove the sealing film on the petri dish, cut ...
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