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Beauveria bassiana and application thereof

A technology of Beauveria bassiana and spores, applied in application, fungi, biocides, etc., can solve problems such as polluting the environment, drug resistance of tea aphids, and affecting the quality of tea leaves.

Active Publication Date: 2019-12-20
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of a large number of pesticides will not only cause the tea aphid to develop resistance, but also pollute the environment and affect the quality of tea. Therefore, it is imminent to explore and develop safe and effective green prevention and control

Method used

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  • Beauveria bassiana and application thereof
  • Beauveria bassiana and application thereof
  • Beauveria bassiana and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] (1) Screening of pathogenic strains

[0094] (1) Beauveria bassiana isolated from aphids and Hemiptera insects provided by the Entomogenic Fungi Research Center of the Key Laboratory of Microbial Control of Anhui Agricultural University was selected for virulence screening; the basic information of the strains is shown in Table 1;

[0095] Table 1 is the source and collection place of Beauveria bassiana

[0096]

[0097] (2) Preparation of spore suspension of Beauveria bassiana: the strain was inoculated on the prepared SDAY medium, placed in an artificial intelligence light incubator (photoperiod 14L:10D) at 25°C for 10 days, and waited for sufficient spore production. Prepare a 50ml Erlenmeyer flask and fill it with 20ml TW-80 solution with a concentration of 0.05%. After sterilization and cooling, use a spore shovel to scrape the spore powder on the medium into it, and shake it on a vortex magnetic oscillator at a frequency of 150r / min for 15min , the spores are ...

Embodiment 2

[0114] (1) Effect of culture medium on sporulation of Beauveria bassiana Bb2032

[0115] (1) Use a 0-100ul adjustable pipette gun to draw 100ul with a concentration of 1×10 6 The spore suspension of highly pathogenic bacterial strains with spores / ml was received on each dish, and the spore suspension was evenly coated with a coating rod, and after standing for 2 minutes, it was sealed with a parafilm and placed in a 25°C artificial intelligence light incubator (light Period 14L: 10D) cultured for 10 days, each medium was a treatment, and each treatment was repeated five times. After cultivation, five holes were punched in each dish with a puncher with a diameter of 4 mm, and the punching positions were the middle and the middle to the middle. At 1 / 2 of the line connecting the edges of the four directions of the dish, put the removed bacteria together with the culture medium into an Erlenmeyer flask filled with 30ml of TW-80 solution with a concentration of 0.05%. Vibrate at a...

Embodiment 3

[0125] (1) Effect of culture temperature on spore germination of Beauveria bassiana Bb2032

[0126] (1) Prepare water agar (WA) medium (dissolve 20g of agar in 1000ml of water, sterilize at 120°C for 20min, add 2ml per dish into a disposable sterile petri dish (D=40mm) when cooled to about 50°C cooling, standby);

[0127] (2) Use a 0.2-2ul adjustable pipette to draw 1ul with a concentration of 1×10 6 The spore suspension of highly pathogenic strains with spores / ml was planted in the middle of the petri dish, sealed with parafilm after standing for 2 minutes, and placed in 18°C, 22°C, 25°C, 28°C, 30°C, 35°C respectively In the artificial intelligence constant temperature incubator, each temperature is a treatment, and each treatment has four groups of repetitions;

[0128] (3) When cultured for 12h and 24h, the spore germination rate of 2 replicates was counted for each treatment respectively. When using an inverted microscope, remove the sealing film on the petri dish, cut ...

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Abstract

The invention discloses Beauveria bassiana, and relates to the technical field of biological pest control. The name of the strain is Beauveria bassiana Bb2032, the preservation number of the strain isCGMCC No.18118, and the preservation date of the strain is August 12, 2019. The invention also provides a method for promoting the germination of Beauveria bassiana Bb2032 spores, a tea aphid prevention and treatment microbial inoculum, a preparation method of the tea aphid prevention and treatment microbial inoculum, and application of Beauveria bassiana in prevention and treatment of tea aphids. The invention has the beneficial effects that: the 8d-fatality rate of the Beauveria bassiana Bb2032 on adult tea aphids and nymphs reaches 90% or above, and the highest 8d-cumulative death rates onthe adult aphids and the nymphs are 100% and 96.23% respectively.

Description

technical field [0001] The invention relates to the technical field of pest biological control, in particular to a strain of Beauveria bassiana and its application. Background technique [0002] The tea aphid, also known as the citrus aphid, is widely distributed in various tea producing areas in my country, and it also harms plants such as camellia oleifera and citrus. The insects do damage by piercing and sucking. Both adults and nymphs have winged and wingless forms. They have strong reproductive ability and fast growth, and are one of the important pests in tea gardens. In my country, there are 20-30 generations of tea aphids per year, about 20 generations in Zhejiang, more than 25 generations in Anhui, and 30 generations in Fujian. One generation is usually completed in 15-20 days in spring tea season, and about 6 in summer. -8d. The saliva secreted by the tea aphid into the tea tree during the process of sucking the host's juice will destroy the normal physiological m...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
CPCC12N1/14C12N1/145C12R2001/645
Inventor 朱虹黄勃陈伟吴昊黎德安袁媛胡四海卫书霞
Owner ANHUI AGRICULTURAL UNIVERSITY
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