Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Insect chitin synthetase 1 gene segment, dsRNA and application thereof

A technology of chitin synthase and gene fragments, applied in the direction of DNA/RNA fragments, applications, recombinant DNA technology, etc., can solve the problems of increased dosage, increased cost of control, and long time for killing insects, etc.

Inactive Publication Date: 2010-09-01
SHANXI UNIV
View PDF3 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The damage caused by agricultural pests is an important factor restricting agricultural production in my country. Long-term application of chemical insecticides leads to a series of problems: 1) Insects become resistant to insecticides, the dosage of pesticides increases, and the cost of control increases; 2) Pesticide residues lead to serious environmental pollution; 3) have a greater impact on non-target organisms
Existing biopesticides are widely used in pest control, but the killing time is long and the effect is slow

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Insect chitin synthetase 1 gene segment, dsRNA and application thereof
  • Insect chitin synthetase 1 gene segment, dsRNA and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Acquisition of chitin synthase 1 gene fragment and dsRNA of migratory locust

[0014] 1) Acquisition of Chitin Synthase 1 Gene Fragment of East Asian Migratory Locust

[0015] According to the common part of the two chitin synthase gene sequences with accession numbers GU067730 and GU067731 in the NCBI database, specific primers were designed using primer premier5.0 software. The sequence of the upstream primer is SEQ ID NO: 2, and the sequence of the downstream primer is SEQ ID NO: 3, all primers were synthesized by Shanghai Yingwei Jieji Biological Co., Ltd. Select the 5th instar nymphs of migratory locust with equal size, half male and half male, in groups of four, and freeze them in liquid nitrogen for RNA extraction. For the specific operation steps of RNA extraction, refer to the TaKaRa Trizol kit. M-MLV reverse transcriptase reverse-transcribed the extracted RNA into the first-strand cDNA, and used this as a template to amplify the chitin synthase 1 g...

Embodiment 2

[0018] Example 2: The dsRNA synthesized by the chitin synthase 1 gene fragment killed the East Asian migratory locust experiment

[0019] 1. Injection of dsRNA synthesized by chitin synthase 1 gene fragment of migratory locust

[0020] The nymphs of the 2nd instar migratory locust with uniform size and health status on the fourth day were selected for injection of the above-mentioned synthetic dsRNA. A 25μl micro-syringe is used for injection. Do not use too much force when injecting. Follow the direction of blood flow. The junction of the 2nd to 3rd abdominal segment of the flank is used as the injection point, avoiding the valve. The amount of dsRNA injected with the chitin synthase 1 gene fragment was 3 μg, and a dsGFP (3 μg) control group was set up, with 15 worms in each group and 3 biological replicates, a total of 45 worms. After the injection, the worms were reared in a 1L beaker in an artificial climate box (light:dark time=14h:10h, temperature 30±2°C, humidity 60%),...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an insect chitin synthetase 1 gene segment, dsRNA and application thereof. The chitin synthetase 1 gene segment of which the sequence is SEQ ID NO:1 is synthesized according to a common part of two chitin synthetase gene sequences with access numbers of GU067730 and GU067731 respectively in an NCBI database; and the dsRNA is synthesized by using the segment. After the synthesized dsRNA is injected into the body cavity of an insect, the insect dies from molting difficulties. Therefore, a new way is provided for the development of a safe and nuisanceless pest preventing and controlling method.

Description

technical field [0001] The present invention relates to the field of biotechnology. It specifically relates to an insect chitin synthase 1 gene fragment and its dsRNA and the application of the dsRNA in killing pests. Background technique [0002] The damage caused by agricultural pests is an important factor restricting agricultural production in my country. Long-term application of chemical insecticides leads to a series of problems: 1) Insects become resistant to insecticides, the dosage of pesticides increases, and the cost of control increases; 2) Pesticide residues lead to serious environmental pollution; 3) Has a greater impact on non-target organisms. Existing biopesticides are widely used in pest control, but the insecticidal time is long and the effect is slow. [0003] RNA interference (RNAi) is a specific post-transcriptional gene silencing phenomenon caused by double-stranded RNA molecules, which won the Nobel Prize in 2006. The discovery of RNAi not only prov...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/52C12N15/113A01N61/00A01P7/04
Inventor 张建珍刘晓健马恩波杨美玲郭亚平
Owner SHANXI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com