Chitin synthase transcriptional regulation gene MedA inactivated aspergillus niger genetically engineered bacterium and application thereof

A technology of genetically engineered bacteria and chitin synthase, applied in the field of genetic engineering, can solve the problems of bacterial residue accumulation, short catalytic aging, easy aging, etc., to increase the effect of oxygen and mass transfer, shorten the fermentation period, and solve the problem of yield. low effect
CN113817616AActive Publication Date: 2021-12-21NANJING UNIV OF TECH +2
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING UNIV OF TECH
Publication Date
2021-12-21

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Abstract

The invention discloses a chitin synthase transcriptional regulation gene MedA inactivated aspergillus niger genetically engineered bacterium and application thereof. According to the invention, an aspergillus niger genetically engineered bacterium with chitin synthase transcriptional regulation gene MedA deletion is constructed through a gene knockout means. According to the genetically engineered bacterium, the biofilm quantity of aspergillus niger in the process of producing citric acid through immobilized fermentation is reduced, the phenomenon that the pore diameter of a carrier is blocked is effectively relieved, the oxygen and mass transfer effect between the carrier and the outside is improved, so that the advantages of immobilized fermentation are exerted, the yield of citric acid is increased, the fermentation period is shortened, and the problems of low yield and long fermentation period in citric acid production through aspergillus niger fermentation in existing industrial engineering are effectively solved.
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Description

technical field

[0001] The invention belongs to the technical field of genetic engineering, and in particular relates to an Aspergillus niger genetically engineered bacterium with inactivated chitin synthase transcription regulation gene MedA, a construction method and application thereof. Background technique

[0002] As the most important acid in biochemical products, citric acid is widely used in food, medicine, daily chemical and other industries. It is one of the organic acids with the largest demand and the most widely used in the world. my country is the main producer of citric acid, and its annual output accounts for 53% of the world's total output. At present, the domestic strains producing citric acid are mainly filamentous fungus Aspergillus niger, and the substrates are mainly cassava flour, corn flour and other rough grain products. In recent years, due to the price of food in our country, labor costs have generally risen, followed by a sharp increase in the pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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