Insecticidal toxicity evaluation method of green muscadine fungus and chlorantraniliprole combined utilization

Abstract

The invention discloses an insecticidal toxicity evaluation method of green muscadine fungus and chlorantraniliprole combined utilization, and provides a method for identifying insecticidal efficacy of chemical agent and fungicide combined utilization. The method for identifying the insecticidal efficacy of the chemical agent and the fungicide combined utilization includes the following steps: 1) respectively detecting LC50 values of the chemical agent, the fungicide, and mixture composed of the chemical agent and the fungicide; 2) converting a bacteria agent concentration unit into a chemical agent concentration unit; 3) calculating a co-toxicity coefficient of the chemical agent and fungicide combined utilization, wherein if the co-toxicity coefficient is equal or greater than 100, the insecticidal efficacy of the chemical agent and fungicide combined utilization is equal or greater than an independent effect of the chemical agent or the fungicide, and a synergized effect is achieved, and if the co-toxicity coefficient is less than 100, the insecticidal efficacy of the chemical agent and the fungicide combined utilization is less than the independent effect, and an antagonistic effect is caused. By adopting the method for identifying the insecticidal efficacy of the chemical agent and the fungicide combined utilization, green muscadine fungus bacterial strain, the chlorantraniliprole and migratory locust are used as experimental materials, an uniform unit is used to convert the LC50 values of the green muscadine fungus and the chlorantraniliprole, and then the co-toxicity coefficient is calculated, and an evaluation criterion is set.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for evaluating the insecticidal toxicity of metarhizium anisopliae and chlorantraniliprole. Background technique [0002] Metarhizium anisopliae is an important class of insect endophytic fungi, which can parasitize a variety of insects. The infection process of Metarhizium anisopliae to the host includes adhesion, spore germination, penetration of the insect body, internal development and death, usually within 24 hours, Metarhizium anisopliae can quickly penetrate the body wall of the insect and invade the host body, a week or more time before the insects are lethal. According to relevant field experiments, the application of 2.0E+12 to 1.0E+13 spores / ha of Metarhizium anisopliae oil can kill 80%-98% of locusts after 7 to 21 days. However, the process of killing insects by Metarhizium anisopliae is slow, and the actual application is affected by environmental factors, spr...

AI Technical Summary

Problems solved by technology

[0003] At present, most of the studies on the evaluation of the synergistic effect of the mixed use of Metarhizium anisopliae and chemical pesticides use a single concentration (sublethal dose) of chemical agents mixed with Metarhizium anisopliae. no synergistic effect

However, there is still a lack of standards for evaluating the mixed effect of Metarhizium anisopliae and chemical pesticides, and there is no method to evaluate the combined effect of the two with the co-toxicity coefficient in the research on the mixed use of Metarhizium anisopliae and chemical pesticides.

Images